GRK5 (NM_005308) Human Tagged ORF Clone

CAT#: RC209137

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GRK5 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 5 (GRK5)



  "NM_005308" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 549.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GRK5
Synonyms FP2025; GPRK5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209137 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCAGGGAAGGGGGCGGAGGAAAGCGCA
AAGGGAAAAGCAAGAAGTGGAAAGAAATCCTGAAGTTCCCTCACATTAGCCAGTGTGAAGACCTCCGAAG
GACCATAGACAGAGATTACTGCAGTTTATGTGACAAGCAGCCAATCGGGAGGCTGCTTTTCCGGCAGTTT
TGTGAAACCAGGCCTGGGCTGGAGTGTTACATTCAGTTCCTGGACTCCGTGGCAGAATATGAAGTTACTC
CAGATGAAAAACTGGGAGAGAAAGGGAAGGAAATTATGACCAAGTACCTCACCCCAAAGTCCCCTGTTTT
CATAGCCCAAGTTGGCCAAGACCTGGTCTCCCAGACGGAGGAGAAGCTCCTACAGAAGCCGTGCAAAGAA
CTCTTTTCTGCCTGTGCACAGTCTGTCCACGAGTACCTGAGGGGAGAACCATTCCACGAATATCTGGACA
GCATGTTTTTTGACCGCTTTCTCCAGTGGAAGTGGTTGGAAAGGCAACCGGTGACCAAAAACACTTTCAG
GCAGTATCGAGTGCTAGGAAAAGGGGGCTTCGGGGAGGTCTGTGCCTGCCAGGTTCGGGCCACGGGTAAA
ATGTATGCCTGCAAGCGCTTGGAGAAGAAGAGGATCAAAAAGAGGAAAGGGGAGTCCATGGCCCTCAATG
AGAAGCAGATCCTCGAGAAGGTCAACAGTCAGTTTGTGGTCAACCTGGCCTATGCCTACGAGACCAAGGA
TGCACTGTGCTTGGTCCTGACCATCATGAATGGGGGTGACCTGAAGTTCCACATCTACAACATGGGCAAC
CCTGGCTTCGAGGAGGAGCGGGCCTTGTTTTATGCGGCAGAGATCCTCTGCGGCTTAGAAGACCTCCACC
GTGAGAACACCGTCTACCGAGATCTGAAACCTGAAAACATCCTGTTAGATGATTATGGCCACATTAGGAT
CTCAGACCTGGGCTTGGCTGTGAAGATCCCCGAGGGAGACCTGATCCGCGGCCGGGTGGGCACTGTTGGC
TACATGGCTCCAGAGGTCCTGAACAACCAGAGGTACGGCCTGAGCCCCGACTACTGGGGCCTTGGCTGCC
TCATCTATGAGATGATCGAGGGCCAGTCGCCGTTCCGCGGCCGCAAGGAGAAGGTGAAGCGGGAGGAGGT
GGACCGCCGGGTCCTGGAGACGGAGGAGGTGTACTCCCACAAGTTCTCCGAGGAGGCCAAGTCCATCTGC
AAGATGCTGCTCACGAAAGATGCGAAGCAGAGGCTGGGCTGCCAGGAGGAGGGGGCTGCAGAGGTCAAGA
GACACCCCTTCTTCAGGAACATGAACTTCAAGCGCTTAGAAGCCGGGATGTTGGACCCTCCCTTCGTTCC
AGACCCCCGCGCTGTGTACTGTAAGGACGTGCTGGACATCGAGCAGTTCTCCACTGTGAAGGGCGTCAAT
CTGGACCACACAGACGACGACTTCTACTCCAAGTTCTCCACGGGCTCTGTGTCCATCCCATGGCAAAACG
AGATGATAGAAACAGAATGCTTTAAGGAGCTGAACGTGTTTGGACCTAATGGTACCCTCCCGCCAGATCT
GAACAGAAACCACCCTCCGGAACCGCCCAAGAAAGGGCTGCTCCAGAGACTCTTCAAGCGGCAGCATCAG
AACAATTCCAAGAGTTCGCCCAGCTCCAAGACCAGTTTTAACCACCACATAAACTCAAACCATGTCAGCT
CGAACTCCACCGGAAGCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209137 protein sequence
Red=Cloning site Green=Tags(s)

MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRLLFRQF
CETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKE
LFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGK
MYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGN
PGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVG
YMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSIC
KMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVN
LDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQ
NNSKSSPSSKTSFNHHINSNHVSSNSTGSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005308
ORF Size 1770 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005308.3
RefSeq Size 2575 bp
RefSeq ORF 1773 bp
Locus ID 2869
UniProt ID P34947
Cytogenetics 10q26.11
Domains RGS, pkinase, S_TK_X, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chemokine signaling pathway, Endocytosis
MW 67.8 kDa
Gene Summary This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]

Other Versions

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