PASK (NM_015148) Human Tagged ORF Clone

CAT#: RC209096

PASK (Myc-DDK-tagged)-Human PAS domain containing serine/threonine kinase (PASK)

Product Images

Circular map for RC209096

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PASK (Cat# RC209096, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PASK(Cat# TA500826). Positive lysates LY414744 (100ug) and LC414744 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY414744) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209096 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PASK protein (Cat# TP309096). The protein was produced from HEK293T cells transfected with PASK cDNA clone (Cat# RC209096) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PASK
• Synonyms: PASKIN; STK37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209096 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGGGGGCTTAACAGCCTTTGAAGAGGACCAGAGATGCCTTTCCCAGAGCCTCCCCTTGCCAG
  TGTCAGCAGAGGGCCCAGCTGCACAGACCACTGCTGAGCCCAGCAGGTCGTTTTCCTCAGCCCACAGACA
  CCTGAGCAGAAGGAATGGGCTTTCCAGACTCTGCCAGAGCAGGACAGCGCTCTCTGAAGACAGATGGAGC
  TCCTATTGTCTATCATCACTGGCTGCCCAGAATATTTGTACAAGTAAACTGCACTGCCCTGCTGCCCCTG
  AGCACACGGACCCGTCCGAACCGCGGGGCAGTGTGTCCTGCTGCTCCCTGCTGCGGGGACTGTCCTCAGG
  GTGGTCCTCACCTCTGCTTCCGGCCCCTGTGTGCAACCCTAACAAGGCCATCTTCACGGTGGATGCCAAG
  ACCACAGAGATCCTGGTTGCTAACGACAAAGCTTGCGGGCTCCTGGGGTACAGCAGCCAGGACCTGATTG
  GCCAGAAGCTCACGCAGTTCTTTCTGAGGTCAGATTCTGATGTGGTGGAGGCCCTCAGCGAGGAGCACAT
  GGAGGCCGACGGCCACGCTGCGGTGGTGTTTGGCACGGTGGTGGACATCATCAGCCGTAGTGGGGAGAAG
  ATTCCAGTGTCTGTGTGGATGAAGAGGATGCGGCAGGAGCGCCGCCTATGCTGCGTGGTGGTCCTGGAGC
  CCGTGGAGAGGGTCTCGACCTGGGTCGCTTTCCAGAGCGATGGCACCGTCACGTCATGTGACAGTCTCTT
  TGCTCATCTTCACGGGTACGTGTCTGGGGAGGACGTGGCTGGGCAGCATATCACAGACCTGATCCCTTCT
  GTGCAGCTCCCTCCTTCTGGCCAGCACATCCCAAAGAATCTCAAGATTCAGAGGTCTGTTGGAAGAGCCA
  GGGACGGTACCACCTTCCCTCTGAGCTTAAAGCTGAAATCCCAACCCAGCAGCGAGGAGGCGACCACCGG
  TGAGGCGGCCCCTGTGAGCGGCTACCGGGCATCTGTCTGGGTGTTCTGCACCATCAGTGGCCTCATCACC
  CTCCTGCCGGATGGGACCATCCACGGCATCAACCACAGCTTCGCGCTGACACTGTTTGGTTACGGAAAGA
  CGGAGCTCCTGGGCAAGAATATCACTTTCCTGATTCCTGGTTTCTACAGCTACATGGACCTTGCGTACAA
  CAGCTCATTACAGCTCCCAGACCTGGCCAGCTGCCTGGACGTCGGCAATGAGAGTGGGTGTGGGGAGAGA
  ACCTTGGACCCGTGGCAGGGCCAGGACCCAGCTGAGGGGGGCCAGGATCCAAGGATTAATGTCGTGCTTG
  CTGGTGGCCACGTTGTGCCCCGAGATGAGATCCGGAAGCTGATGGAAAGCCAAGACATCTTCACCGGGAC
  TCAGACTGAGCTGATTGCTGGAGGCCAGCTCCTTTCCTGCCTCTCACCTCAGCCTGCTCCAGGGGTGGAC
  AATGTCCCAGAAGGAAGCCTGCCAGTGCACGGTGAACAGGCGCTGCCCAAGGACCAGCAAATCACTGCCT
  TGGGGAGAGAGGAACCTGTGGCAATAGAGAGCCCCGGACAGGATCTTCTGGGAGAAAGCAGGTCTGAACC
  AGTGGATGTGAAGCCATTTGCTTCCTGCGAAGATTCTGAAGCTCCAGTCCCAGCTGAGGATGGGGGCAGT
  GATGCTGGCATGTGTGGCCTGTGTCAGAAGGCCCAGCTAGAGCGGATGGGAGTCAGTGGTCCCAGCGGTT
  CAGACCTTTGGGCTGGGGCTGCCGTGGCCAAGCCCCAGGCCAAGGGTCAGCTGGCGGGGGGCAGCCTCCT
  GATGCACTGCCCTTGCTATGGGAGTGAATGGGGCTTGTGGTGGCGAAGCCAGGACTTGGCCCCCAGCCCC
  TCTGGGATGGCAGGCCTCTCGTTTGGGACACCTACTCTAGATGAGCCGTGGCTGGGAGTGGAAAACGACC
  GAGAAGAGCTGCAGACCTGCTTGATTAAGGAGCAGCTGTCCCAGTTGAGCCTTGCAGGAGCCCTGGATGT
  CCCCCACGCCGAACTCGTTCCGACAGAGTGCCAGGCTGTCACCGCTCCTGTGTCGTCCTGCGATCTGGGA
  GGCAGAGACCTGTGCGGTGGCTGCACGGGCAGCTCCTCAGCCTGCTATGCCTTGGCCACGGACCTCCCTG
  GGGGCCTGGAAGCAGTGGAGGCCCAGGAGGTTGATGTGAATTCGTTTTCCTGGAACCTCAAGGAACTCTT
  TTTCAGTGACCAGACAGACCAAACGTCATCAAATTGTTCCTGTGCTACGTCTGAACTCAGAGAGACACCC
  TCTTCCTTGGCAGTGGGCTCCGATCCAGATGTAGGCAGTCTCCAGGAACAGGGGTCGTGTGTCCTGGATG
  ACAGGGAGCTGTTACTACTGACCGGCACCTGTGTTGACCTTGGCCAAGGCCGACGGTTCCGGGAGAGCTG
  TGTGGGACATGATCCAACAGAACCGCTTGAGGTTTGTTTGGTGTCCTCTGAGCATTATGCAGCAAGCGAC
  AGAGAAAGCCCAGGACACGTTCCTTCCACGTTGGATGCTGGCCCTGAGGACACGTGCCCATCAGCAGAGG
  AGCCAAGGCTGAACGTCCAGGTCACCTCCACGCCCGTGATCGTGATGCGCGGGGCTGCTGGCCTGCAGCG
  GGAGATCCAGGAGGGTGCCTACTCCGGGAGCTGCTACCATCGAGATGGCTTACGGCTGAGTATACAGTTT
  GAGGTGAGGCGGGTGGAGCTCCAGGGCCCCACACCTCTGTTCTGCTGCTGGCTGGTGAAAGACCTCCTCC
  ACAGCCAACGCGACTCAGCCGCCAGGACCCGCCTGTTCCTTGCCAGCCTGCCCGGCTCCACCCACTCTAC
  CGCTGCTGAGCTCACCGGACCCAGCCTGGTGGAAGTGCTCAGAGCCAGACCCTGGTTTGAGGAGCCCCCC
  AAGGCTGTGGAACTGGAGGGGTTGGCGGCCTGTGAGGGCGAGTACTCCCAAAAGTACAGTACCATGAGCC
  CGCTGGGCAGTGGGGCCTTCGGCTTCGTGTGGACTGCTGTGGACAAGGAAAAAAACAAGGAGGTGGTGGT
  GAAGTTTATTAAGAAGGAGAAGGTCTTGGAGGATTGTTGGATTGAGGATCCCAAACTTGGGAAAGTTACT
  TTAGAGATCGCAATTCTATCCAGGGTGGAGCACGCCAATATCATCAAGGTATTGGATATATTTGAAAACC
  AAGGGTTCTTCCAGCTTGTGATGGAGAAGCACGGCTCCGGCCTAGACCTCTTCGCTTTCATCGACCGCCA
  CCCCAGGCTGGATGAGCCCCTGGCGAGCTACATCTTCCGACAACTAGTGTCAGCAGTGGGATACCTGCGC
  TTGAAGGACATCATCCACCGTGACATCAAGGATGAGAACATCGTGATCGCCGAGGACTTCACAATCAAGC
  TGATAGACTTTGGCTCGGCCGCCTACTTGGAAAGGGGAAAATTATTTTATACTTTTTGTGGGACCATCGA
  GTACTGTGCACCGGAAGTTCTCATGGGGAATCCCTACAGAGGGCCGGAGCTGGAGATGTGGTCTCTGGGA
  GTCACTCTGTACACGCTGGTCTTTGAGGAGAACCCCTTCTGTGAGCTGGAGGAGACCGTGGAGGCTGCCA
  TACACCCGCCATACCTGGTGTCCAAAGAACTCATGAGCCTTGTGTCTGGGCTGCTGCAGCCAGTCCCTGA
  GAGACGCACCACCTTGGAGAAGCTGGTGACAGACCCGTGGGTAACACAGCCTGTGAATCTTGCTGACTAT
  ACATGGGAAGAGGTGTTTCGAGTAAACAAGCCAGAAAGTGGAGTTCTGTCCGCTGCGAGCCTGGAGATGG
  GGAACAGGAGCCTGAGTGATGTGGCCCAGGCTCAGGAGCTTTGTGGGGGCCCCGTTCCAGGCGAGGCTCC
  TAATGGCCAAGGCTGTTTGCATCCCGGGGATCCCCGTCTGCTGACCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209096 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSAHRHLSRRNGLSRLCQSRTALSEDRWS
  SYCLSSLAAQNICTSKLHCPAAPEHTDPSEPRGSVSCCSLLRGLSSGWSSPLLPAPVCNPNKAIFTVDAK
  TTEILVANDKACGLLGYSSQDLIGQKLTQFFLRSDSDVVEALSEEHMEADGHAAVVFGTVVDIISRSGEK
  IPVSVWMKRMRQERRLCCVVVLEPVERVSTWVAFQSDGTVTSCDSLFAHLHGYVSGEDVAGQHITDLIPS
  VQLPPSGQHIPKNLKIQRSVGRARDGTTFPLSLKLKSQPSSEEATTGEAAPVSGYRASVWVFCTISGLIT
  LLPDGTIHGINHSFALTLFGYGKTELLGKNITFLIPGFYSYMDLAYNSSLQLPDLASCLDVGNESGCGER
  TLDPWQGQDPAEGGQDPRINVVLAGGHVVPRDEIRKLMESQDIFTGTQTELIAGGQLLSCLSPQPAPGVD
  NVPEGSLPVHGEQALPKDQQITALGREEPVAIESPGQDLLGESRSEPVDVKPFASCEDSEAPVPAEDGGS
  DAGMCGLCQKAQLERMGVSGPSGSDLWAGAAVAKPQAKGQLAGGSLLMHCPCYGSEWGLWWRSQDLAPSP
  SGMAGLSFGTPTLDEPWLGVENDREELQTCLIKEQLSQLSLAGALDVPHAELVPTECQAVTAPVSSCDLG
  GRDLCGGCTGSSSACYALATDLPGGLEAVEAQEVDVNSFSWNLKELFFSDQTDQTSSNCSCATSELRETP
  SSLAVGSDPDVGSLQEQGSCVLDDRELLLLTGTCVDLGQGRRFRESCVGHDPTEPLEVCLVSSEHYAASD
  RESPGHVPSTLDAGPEDTCPSAEEPRLNVQVTSTPVIVMRGAAGLQREIQEGAYSGSCYHRDGLRLSIQF
  EVRRVELQGPTPLFCCWLVKDLLHSQRDSAARTRLFLASLPGSTHSTAAELTGPSLVEVLRARPWFEEPP
  KAVELEGLAACEGEYSQKYSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVT
  LEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLR
  LKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLG
  VTLYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADY
  TWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRLLTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015148
• ORF Size: 3969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015148.4
• RefSeq Size: 4588 bp
• RefSeq ORF: 3972 bp
• Locus ID: 23178
• UniProt ID: Q96RG2
• Cytogenetics: 2q37.3
• Domains: S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• MW: 142.9 kDa
• Gene Summary: This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]