RPL41 (NM_021104) Human Tagged ORF Clone

CAT#: RC209076

RPL41 (Myc-DDK-tagged)-Human ribosomal protein L41 (RPL41), transcript variant 1

Product Images

Circular map for RC209076

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPL41
• Synonyms: L41
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209076 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATTTTGTTTATGAGCAAGGTGGGTCTCAGAGGTGATCGGCGATCAGAGGGCGATGAAGTTCTAG
  ATCCATTGAGACAAGCTCTAGACAGTAGCATGCAGTCCCACAACTTGTACCAGCATCCCCAGCATCTGGC
  ATTCCATGTTTCTGCTCCTGTGGCCTCCACAGTGCAACAAGCTAGCGGTTTACTTGGACCTCTACCTCAT
  CTTTCTTCTTTTGCGCTTCAGCCTGCGCATTCGCTTCTTCCTCCACTTGGCTCTCATGGTGCAGGTTTCC
  AAGAAAATGGCGCTAAGGCCGAGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209076 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGILFMSKVGLRGDRRSEGDEVLDPLRQALDSSMQSHNLYQHPQHLAFHVSAPVASTVQQASGLLGPLPH
  LSSFALQPAHSLLPPLGSHGAGFQENGAKAES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021104
• ORF Size: 309 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 478 bp
• RefSeq ORF: 78 bp
• Locus ID: 6171
• UniProt ID: P62945
• Cytogenetics: 12q13.2
• Protein Pathways: Ribosome
• MW: 10.8 kDa
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which shares sequence similarity with the yeast ribosomal protein YL41, belongs to the L41E family of ribosomal proteins. It is located in the cytoplasm. The protein can interact with the beta subunit of protein kinase CKII and can stimulate the phosphorylation of DNA topoisomerase II-alpha by CKII. Two alternative splice variants have been identified, both encoding the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]