hnRNP L (HNRNPL) (NM_001005335) Human Tagged ORF Clone

CAT#: RC209067

HNRNPL (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY423861) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209067 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HNRNPL
• Synonyms: hnRNP-L; HNRPL; P/OKcl.14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209067 representing NM_001005335
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAAAAAGAGACAAGCACTGGTGGAGTTTGAAGATGTGTTGGGGGCTTGCAACGCAGTGAACTACG
  CAGCCGACAACCAAATATACATTGCTGGTCACCCAGCTTTTGTCAACTACTCTACCAGCCAGAAGATCTC
  CCGCCCTGGGGACTCGGATGACTCCCGGAGCGTGAACAGTGTGCTTCTCTTTACCATCCTGAACCCCATT
  TATTCGATCACCACGGATGTTCTTTACACTATCTGTAATCCTTGTGGCCCTGTCCAGAGAATTGTCATTT
  TCAGGAAGAATGGAGTTCAGGCGATGGTGGAATTTGACTCAGTTCAAAGTGCCCAGCGGGCCAAGGCCTC
  TCTCAATGGGGCTGATATCTATTCTGGCTGTTGCACTCTGAAGATCGAATACGCAAAGCCTACACGCTTG
  AATGTGTTCAAGAATGATCAGGATACTTGGGACTACACAAACCCCAATCTCAGTGGACAAGGTGACCCTG
  GCAGCAACCCCAACAAACGCCAGAGGCAGCCCCCTCTCCTGGGAGATCACCCCGCAGAATATGGAGGGCC
  CCACGGTGGGTACCACAGCCATTACCATGATGAGGGCTACGGGCCCCCCCCACCTCACTACGAAGGGAGA
  AGGATGGGTCCACCAGTGGGGGGTCACCGTCGGGGCCCAAGTCGCTACGGCCCCCAGTATGGGCACCCCC
  CACCCCCTCCCCCACCACCCGAGTATGGCCCTCACGCCGACAGCCCTGTGCTCATGGTCTATGGCTTGGA
  TCAATCTAAGATGAACTGTGACCGAGTCTTCAATGTCTTCTGCTTATATGGCAATGTGGAGAAGGTGAAA
  TTCATGAAAAGCAAGCCGGGGGCCGCCATGGTGGAGATGGCTGATGGCTACGCTGTAGACCGGGCCATTA
  CCCACCTCAACAACAACTTCATGTTTGGGCAGAAGCTGAATGTCTGTGTCTCCAAGCAGCCAGCCATCAT
  GCCTGGTCAGTCATACGGGTTGGAAGACGGGTCTTGCAGTTACAAAGACTTCAGTGAATCCCGGAACAAT
  CGGTTCTCCACCCCAGAGCAGGCAGCCAAGAACCGCATCCAGCACCCCAGCAACGTGCTGCACTTCTTCA
  ACGCCCCGCTGGAGGTGACCGAGGAGAACTTCTTTGAGATCTGCGATGAGCTGGGAGTGAAGCGGCCATC
  TTCTGTGAAAGTATTCTCAGGCAAAAGTGAGCGCAGCTCCTCTGGACTGCTGGAGTGGGAATCCAAGAGC
  GATGCCCTGGAGACTCTGGGCTTCCTGAACCATTACCAGATGAAAAACCCAAATGGTCCATACCCTTACA
  CTCTGAAGTTGTGTTTCTCCACTGCTCAGCACGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209067 representing NM_001005335
  Red=Cloning site Green=Tags(s)
  
  MPKKRQALVEFEDVLGACNAVNYAADNQIYIAGHPAFVNYSTSQKISRPGDSDDSRSVNSVLLFTILNPI
  YSITTDVLYTICNPCGPVQRIVIFRKNGVQAMVEFDSVQSAQRAKASLNGADIYSGCCTLKIEYAKPTRL
  NVFKNDQDTWDYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGR
  RMGPPVGGHRRGPSRYGPQYGHPPPPPPPPEYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVK
  FMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQPAIMPGQSYGLEDGSCSYKDFSESRNN
  RFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPSSVKVFSGKSERSSSGLLEWESKS
  DALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005335
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005335.2
• RefSeq Size: 1895 bp
• RefSeq ORF: 1371 bp
• Locus ID: 3191
• UniProt ID: P14866
• Cytogenetics: 19q13.2
• MW: 50.6 kDa
• Gene Summary: Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]