SEC23IP (NM_007190) Human Tagged ORF Clone

CAT#: RC209056

SEC23IP (Myc-DDK-tagged)-Human SEC23 interacting protein (SEC23IP), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402101) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209056 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SEC23IP protein (Cat# TP309056). The protein was produced from HEK293T cells transfected with SEC23IP cDNA clone (Cat# RC209056) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEC23IP
• Synonyms: iPLA1beta; MSTP053; P125; P125A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209056 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAGAAAACCTAACGGTGGCAGCGGCGGCGCCTCCACTTCCTCATCGGGCACTAACTTACTTT
  TCTCCTCCTCGGCCACGGAGTTCAGCTTCAATGTGCCCTTCATCCCAGTCACCCAGGCCTCCGCTTCTCC
  GGCCTCCCTGCTCTTACCGGGAGAGGATTCCACAGATGTTGGTGAGGAGGACAGCTTCCTTGGTCAGACT
  TCTATTCACACATCTGCCCCACAGACATTTAGTTACTTCTCTCAGGTATCAAGCAGCAGTGATCCTTTTG
  GGAATATTGGACAGTCACCATTAACAACTGCAGCAACCTCAGTTGGACAATCAGGATTCCCCAAGCCCCT
  GACTGCTCTCCCTTTTACAACTGGATCCCAAGATGTCTCGAATGCATTTTCACCATCCATTTCGAAGGCT
  CAACCTGGTGCTCCACCTTCCTCACTGATGGGAATAAATTCTTATCTGCCTTCTCAGCCAAGTAGTCTCC
  CTCCTTCATATTTTGGGAACCAACCCCAAGGAATTCCCCAACCAGGATACAATCCATATCGCCATACCCC
  TGGCAGCAGCAGGGCTAATCCTTACATTGCACCACCCCAGCTGCAGCAGTGCCAAACACCAGGCCCTCCT
  GCTCATCCTCCACCTTCTGGACCCCCTGTTCAGATGTACCAGATGCCTCCAGGATCTTTGCCACCGGTTC
  CTTCTTCAGTGCAGTCACCGGCACAGCAGCAGGTACCTGCCAGACCTGGGGCTCCCTCTGTTCAAGTGCC
  ATCTCCTTTTCTACTTCAAAACCAATATGAGCCTGTTCAGCCCCACTGGTTTTACTGCAAGGAGGTAGAA
  TACAAACAACTGTGGATGCCTTTTAGTGTGTTCGACTCTTTGAATCTTGAAGAAATCTATAATTCAGTTC
  AGCCAGATCCGGAGAGCGTGGTTCTTGGCACGGATGGAGGGCGCTACGATGTTTACCTCTATGACCGAAT
  AAGGAAGGCTGCCTACTGGGAAGAGGAGCCAGCCGAAGTGAGACGCTGTACTTGGTTTTACAAGGGGGAC
  ACAGATAGTCGATTTATTCCCTATACTGAGGAGTTCAGTGAAAAACTAGAGGCTGAATATAAAAAAGCTG
  TAACCACTAATCAGTGGCACCGAAGATTAGAGTTTCCAAGTGGAGAGACAATTGTTATGCACAATCCAAA
  GGTTATTGTTCAGTTCCAGCCCTCCTCAGTGCCAGATGAATGGGGCACCACGCAAGATGGACAGACAAGG
  CCCAGGGTTGTAAAGCGTGGAATTGATGATAACCTTGATGAAATTCCCGACGGGGAGATGCCTCAAGTTG
  ACCATTTGGTGTTTGTGGTGCATGGCATTGGACCTGTGTGTGACTTACGCTTTAGGAGCATTATTGAGTG
  TGTGGATGATTTTAGGGTGGTTTCTCTCAAATTGCTGCGGACACATTTCAAGAAATCTTTAGATGACGGG
  AAAGTAAGCAGAGTGGAGTTCCTTCCAGTTCATTGGCATAGTTCTTTGGGTGGGGACGCCACAGGTGTGG
  ACAGGAATATTAAGAAAATCACTTTGCCAAGTATTGGTCGATTTCGTCACTTTACCAATGAAACTTTGCT
  AGATATTTTATTTTATAACAGCCCCACCTACTGTCAGACAATTGTGGAAAAAGTAGGAATGGAGATAAAC
  CATCTGCATGCACTCTTTATGAGTCGGAACCCAGACTTCAAAGGAGGTGTCTCTGTTGCTGGTCACAGTT
  TAGGTTCTTTAATATTGTTTGACATCCTGTCTAATCAAAAAGATTTGAATTTATCAAAGTGCCCTGGACC
  TCTTGCTGTTGCTAATGGAGTTGTGAAGCAGCTACATTTTCAGGAAAAGCAGATGCCTGAAGAGCCAAAG
  CTGACTTTGGATGAGTCGTATGACCTTGTTGTTGAAAATAAAGAAGTCCTAACTTTGCAAGAAACTCTGG
  AAGCACTTAGCCTCTCTGAATATTTTAGCACTTTTGAAAAGGAAAAGATTGATATGGAGTCCCTGCTTAT
  GTGTACAGTTGATGACCTGAAGGAAATGGGGATACCCCTTGGACCCAGAAAGAAGATAGCTAACTTTGTA
  GAACATAAAGCAGCCAAACTGAAAAAAGCAGCGTCAGAAAAGAAGGCAGTGGCGGCCACTTCTACAAAAG
  GACAAGAGCAAAGTGCCCAGAAGACTAAAGACATGGCTTCCCTCCCCTCAGAATCCAATGAGCCAAAGAG
  GAAACTTCCAGTTGGTGCTTGCGTGTCTTCTGTGTGTGTGAATTATGAATCTTTTGAAGTTGGCGCCGGA
  CAGGTTTCTGTTGCTTACAACTCATTAGATTTTGAACCAGAGATATTCTTTGCCTTGGGGTCTCCAATTG
  CTATGTTTCTCACTATTCGAGGAGTTGATAGGATAGATGAGAATTACAGCCTTCCTACCTGTAAAGGGTT
  CTTCAATATTTATCATCCGCTTGATCCAGTGGCATATAGATTAGAACCTATGATTGTTCCAGATTTGGAC
  CTAAAAGCTGTTCTCATTCCACATCACAAAGGCAGAAAAAGACTTCATTTAGAATTGAAAGAGAGTCTCT
  CTCGTATGGGATCTGATTTGAAGCAGGGTTTTATTAGCTCTCTCAAAAGTGCTTGGCAGACATTAAATGA
  GTTTGCCCGTGCTCATACGTCTTCAACCCAGTTGCAAGAAGAATTGGAGAAGGTGGCCAATCAGATCAAA
  GAAGAAGAAGAAAAGCAAGTAGTTGAAGCAGAAAAGGTTGTTGAAAGTCCAGATTTTTCCAAGGATGAGG
  ACTACTTAGGAAAGGTTGGAATGTTAAATGGAGGCCGCCGAATTGACTACGTTCTCCAAGAAAAACCAAT
  AGAGAGTTTTAATGAATACCTTTTCGCTCTTCAGAGTCACTTATGCTATTGGGAATCTGAAGATACTGCT
  CTGTTACTACTTAAAGAAATTTATCGAACAATGAACATTAGTCCAGAACAGCCCCAGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209056 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAERKPNGGSGGASTSSSGTNLLFSSSATEFSFNVPFIPVTQASASPASLLLPGEDSTDVGEEDSFLGQT
  SIHTSAPQTFSYFSQVSSSSDPFGNIGQSPLTTAATSVGQSGFPKPLTALPFTTGSQDVSNAFSPSISKA
  QPGAPPSSLMGINSYLPSQPSSLPPSYFGNQPQGIPQPGYNPYRHTPGSSRANPYIAPPQLQQCQTPGPP
  AHPPPSGPPVQMYQMPPGSLPPVPSSVQSPAQQQVPARPGAPSVQVPSPFLLQNQYEPVQPHWFYCKEVE
  YKQLWMPFSVFDSLNLEEIYNSVQPDPESVVLGTDGGRYDVYLYDRIRKAAYWEEEPAEVRRCTWFYKGD
  TDSRFIPYTEEFSEKLEAEYKKAVTTNQWHRRLEFPSGETIVMHNPKVIVQFQPSSVPDEWGTTQDGQTR
  PRVVKRGIDDNLDEIPDGEMPQVDHLVFVVHGIGPVCDLRFRSIIECVDDFRVVSLKLLRTHFKKSLDDG
  KVSRVEFLPVHWHSSLGGDATGVDRNIKKITLPSIGRFRHFTNETLLDILFYNSPTYCQTIVEKVGMEIN
  HLHALFMSRNPDFKGGVSVAGHSLGSLILFDILSNQKDLNLSKCPGPLAVANGVVKQLHFQEKQMPEEPK
  LTLDESYDLVVENKEVLTLQETLEALSLSEYFSTFEKEKIDMESLLMCTVDDLKEMGIPLGPRKKIANFV
  EHKAAKLKKAASEKKAVAATSTKGQEQSAQKTKDMASLPSESNEPKRKLPVGACVSSVCVNYESFEVGAG
  QVSVAYNSLDFEPEIFFALGSPIAMFLTIRGVDRIDENYSLPTCKGFFNIYHPLDPVAYRLEPMIVPDLD
  LKAVLIPHHKGRKRLHLELKESLSRMGSDLKQGFISSLKSAWQTLNEFARAHTSSTQLQEELEKVANQIK
  EEEEKQVVEAEKVVESPDFSKDEDYLGKVGMLNGGRRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTA
  LLLLKEIYRTMNISPEQPQH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007190
• ORF Size: 3000 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007190.4
• RefSeq Size: 7306 bp
• RefSeq ORF: 3003 bp
• Locus ID: 11196
• UniProt ID: Q9Y6Y8
• Cytogenetics: 10q26.11-q26.12
• Domains: SAM, DDHD
• MW: 111.1 kDa
• Gene Summary: This gene encodes a member of the phosphatidic acid preferring-phospholipase A1 family. The encoded protein is localized to endoplasmic reticulum exit sites and plays a critical role in ER-Golgi transport as part of the multimeric coat protein II complex. An orthologous gene in frogs is required for normal neural crest cell development, suggesting that this gene may play a role in Waardenburg syndrome neural crest defects. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]