ABCB10 (NM_012089) Human Tagged ORF Clone

CAT#: RC209046

ABCB10 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 10 (ABCB10), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY415992) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209046 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCB10
• Synonyms: EST20237; M-ABC2; MTABC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209046 representing NM_012089
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGGCCCCCCTGCCTGGCCGCTGCGGCTGCTCGAGCCACCGAGCCCTGCCGAGCCAGGTCGGCTCC
  TGCCGGTAGCCTGCGTGTGGGCCGCGGCCAGCCGCGTTCCCGGGTCCCTATCGCCGTTCACTGGCCTGAG
  GCCGGCGCGGCTATGGGGCGCGGGGCCCGCGCTGCTCTGGGGCGTTGGAGCCGCGCGCCGCTGGAGGAGC
  GGCTGCCGGGGCGGGGGTCCGGGCGCCTCGCGGGGCGTCCTGGGCCTCGCGCGGCTCCTGGGGCTGTGGG
  CTCGCGGCCCCGGCAGCTGCAGGTGCGGGGCTTTTGCCGGGCCAGGCGCTCCTCGGCTCCCGCGCGCCCG
  GTTCCCGGGCGGTCCCGCAGCCGCTGCCTGGGCAGGGGACGAGGCCTGGCGGCGCGGGCCGGCGGCGCCT
  CCCGGGGACAAGGGGCGGCTGCGCCCCGCAGCGGCCGGACTCCCGGAGGCCCGGAAGCTCCTGGGGCTGG
  CGTACCCTGAGCGCCGGAGGCTGGCAGCTGCGGTTGGATTTCTCACGATGTCCAGTGTTATCTCCATGTC
  TGCCCCTTTCTTCCTGGGGAAGATCATTGATGTCATCTATACCAACCCCACTGTGGACTACAGCGACAAC
  CTGACCCGCCTCTGCCTAGGGCTCAGTGCCGTGTTTCTGTGTGGTGCTGCCGCCAATGCCATTCGTGTCT
  ACCTCATGCAAACTTCAGGTCAGCGCATTGTGAATAGGCTGAGAACTTCATTATTCTCCTCCATTCTGAG
  GCAGGAGGTTGCTTTCTTTGACAAGACTCGCACAGGAGAATTGATTAACCGCCTCTCATCAGACACTGCA
  CTCCTGGGGCGCTCAGTGACTGAAAACCTCTCAGATGGGCTCAGGGCCGGGGCCCAGGCTTCTGTAGGCA
  TCAGTATGATGTTTTTTGTCTCACCTAATCTGGCCACCTTTGTTTTGAGCGTGGTGCCTCCAGTGTCAAT
  CATTGCTGTAATTTATGGGCGATATCTACGGAAACTGACCAAAGTCACTCAGGATTCCCTGGCACAAGCC
  ACTCAGCTAGCTGAGGAACGTATTGGAAATGTAAGAACTGTTCGAGCTTTTGGGAAAGAAATGACTGAAA
  TCGAGAAATATGCCAGCAAAGTGGACCATGTAATGCAGTTAGCAAGGAAAGAGGCATTCGCCCGGGCTGG
  TTTCTTTGGAGCAACTGGGCTCTCCGGAAACCTGATCGTGCTTTCTGTCCTGTACAAAGGAGGGCTGCTG
  ATGGGCAGTGCCCACATGACCGTGGGTGAACTCTCTTCCTTCCTAATGTATGCTTTCTGGGTTGGAATAA
  GCATTGGAGGTCTGAGCTCTTTCTACTCGGAGCTGATGAAAGGACTGGGTGCAGGGGGGCGCCTCTGGGA
  GCTCCTGGAGAGAGAGCCCAAGCTGCCTTTTAACGAGGGGGTCATCTTAAATGAGAAAAGCTTCCAGGGT
  GCTTTGGAGTTTAAGAACGTGCATTTTGCCTATCCAGCTCGCCCAGAGGTGCCCATATTTCAGGATTTCA
  GCCTTTCCATTCCGTCAGGATCTGTCACGGCACTGGTTGGCCCAAGTGGTTCTGGCAAATCAACAGTGCT
  TTCACTCCTGCTGAGGTTGTACGACCCTGCTTCTGGAACTATTAGTCTTGATGGCCATGACATCCGTCAG
  CTAAACCCAGTGTGGCTGAGATCCAAAATTGGGACAGTGAGTCAGGAACCCATTTTGTTTTCTTGCTCTA
  TTGCTGAGAACATTGCTTATGGTGCTGATGACCCTTCCTCTGTGACCGCTGAGGAAATCCAGAGAGTGGC
  TGAAGTGGCCAATGCAGTGGCCTTCATCCGGAATTTCCCCCAAGGGTTCAACACTGTGGTTGGAGAAAAG
  GGTGTTCTCCTCTCAGGTGGGCAGAAACAGCGGATTGCGATTGCCCGTGCTCTGCTAAAGAATCCCAAAA
  TTCTTCTCCTAGATGAAGCAACCAGTGCGCTGGATGCCGAAAATGAGTACCTTGTTCAAGAAGCTCTAGA
  TCGACTGATGGATGGAAGAACGGTGTTAGTTATTGCCCATCGTCTGTCCACCATTAAGAATGCTAATATG
  GTTGCTGTTCTTGACCAAGGAAAAATTACTGAATATGGAAAACATGAAGAGCTGCTTTCAAAACCAAATG
  GGATATACAGAAAACTAATGAACAAACAAAGTTTTATTTCAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209046 representing NM_012089
  Red=Cloning site Green=Tags(s)
  
  MRGPPAWPLRLLEPPSPAEPGRLLPVACVWAAASRVPGSLSPFTGLRPARLWGAGPALLWGVGAARRWRS
  GCRGGGPGASRGVLGLARLLGLWARGPGSCRCGAFAGPGAPRLPRARFPGGPAAAAWAGDEAWRRGPAAP
  PGDKGRLRPAAAGLPEARKLLGLAYPERRRLAAAVGFLTMSSVISMSAPFFLGKIIDVIYTNPTVDYSDN
  LTRLCLGLSAVFLCGAAANAIRVYLMQTSGQRIVNRLRTSLFSSILRQEVAFFDKTRTGELINRLSSDTA
  LLGRSVTENLSDGLRAGAQASVGISMMFFVSPNLATFVLSVVPPVSIIAVIYGRYLRKLTKVTQDSLAQA
  TQLAEERIGNVRTVRAFGKEMTEIEKYASKVDHVMQLARKEAFARAGFFGATGLSGNLIVLSVLYKGGLL
  MGSAHMTVGELSSFLMYAFWVGISIGGLSSFYSELMKGLGAGGRLWELLEREPKLPFNEGVILNEKSFQG
  ALEFKNVHFAYPARPEVPIFQDFSLSIPSGSVTALVGPSGSGKSTVLSLLLRLYDPASGTISLDGHDIRQ
  LNPVWLRSKIGTVSQEPILFSCSIAENIAYGADDPSSVTAEEIQRVAEVANAVAFIRNFPQGFNTVVGEK
  GVLLSGGQKQRIAIARALLKNPKILLLDEATSALDAENEYLVQEALDRLMDGRTVLVIAHRLSTIKNANM
  VAVLDQGKITEYGKHEELLSKPNGIYRKLMNKQSFISA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012089
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012089.3
• RefSeq Size: 3857 bp
• RefSeq ORF: 2217 bp
• Locus ID: 23456
• UniProt ID: Q9NRK6
• Cytogenetics: 1q42.13
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 79 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The function of this mitochondrial protein is unknown. [provided by RefSeq, Jul 2008]