FENS1 (WDFY1) (NM_020830) Human Tagged ORF Clone

CAT#: RC209030

WDFY1 (Myc-DDK-tagged)-Human WD repeat and FYVE domain containing 1 (WDFY1)

Product Images

Circular map for RC209030

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  Western blot validation of overexpression lysate (Cat# LY402807) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209030 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDFY1 protein (Cat# TP309030). The protein was produced from HEK293T cells transfected with WDFY1 cDNA clone (Cat# RC209030) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FENS1
• Synonyms: FENS-1; FENS1; WDF1; ZFYVE17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209030 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGAAATCCACTCCAGGCCGCAGAGCAGCCGCCCGGTGCTGCTGAGCAAGATCGAGGGGCACC
  AGGACGCCGTCACGGCCGCGCTGCTCATCCCCAAGGAGGACGGCGTGATCACGGCCAGCGAGGACAGAAC
  CATCCGGGTATGGCTGAAAAGAGACAGTGGTCAATACTGGCCCAGCATTTACCACACAATGGCCTCTCCT
  TGCTCTGCTATGGCTTACCATCATGACAGCAGACGGATATTTGTGGGCCAGGATAATGGAGCTGTAATGG
  AATTTCACGTTTCTGAAGATTTTAATAAAATGAACTTTATCAAGACCTACCCAGCTCATCAGAACCGGGT
  GTCTGCGATTATCTTCAGCTTGGCCACAGAGTGGGTGATCAGTACCGGCCACGACAAGTGTGTGAGCTGG
  ATGTGCACGCGGAGCGGGAACATGCTCGGGAGGCACTTCTTCACGTCCTGGGCTTCGTGTCTGCAATATG
  ACTTTGACACTCAGTATGCTTTCGTTGGTGATTATTCTGGGCAGATCACCCTGCTGAAGCTTGAACAGAA
  CACGTGTTCAGTCATCACAACCCTCAAAGGACATGAAGGTAGTGTCGCCTGCCTCTGGTGGGACCCTATT
  CAGCGGTTACTCTTCTCAGGAGCATCTGACAACAGCATCATCATGTGGGACATCGGAGGAAGGAAAGGCC
  GGACGCTGTTACTTCAGGGCCATCATGACAAGGTGCAGTCGCTGTGCTACCTTCAGCTCACCAGGCAGCT
  CGTCTCCTGTTCCTCGGACGGCGGAATTGCAGTGTGGAACATGGATGTTAGCAGAGAAGAGGCTCCTCAG
  TGGTTGGAAAGTGATTCTTGTCAGAAATGTGAGCAGCCATTTTTCTGGAACATAAAGCAGATGTGGGACA
  CCAAGACGCTGGGGCTAAGACAACATCACTGCAGGAAATGCGGGCAGGCTGTCTGCGGGAAGTGCAGCAG
  CAAGCGCTCAAGTTACCCAGTCATGGGCTTCGAGTTCCAAGTCCGGGTTTGTGATTCTTGTTACGACTCC
  ATCAAAGATGAAGATCGGACTTCTCTAGCGACCTTTCATGAAGGAAAACATAACATTTCCCACATGTCCA
  TGGACATTGCCAGGGGACTGATGGTGACCTGTGGGACCGACCGCATTGTAAAGATCTGGGACATGACACC
  TGTGGTGGGCTGCAGTCTGGCGACTGGGTTTTCTCCGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209030 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAEIHSRPQSSRPVLLSKIEGHQDAVTAALLIPKEDGVITASEDRTIRVWLKRDSGQYWPSIYHTMASP
  CSAMAYHHDSRRIFVGQDNGAVMEFHVSEDFNKMNFIKTYPAHQNRVSAIIFSLATEWVISTGHDKCVSW
  MCTRSGNMLGRHFFTSWASCLQYDFDTQYAFVGDYSGQITLLKLEQNTCSVITTLKGHEGSVACLWWDPI
  QRLLFSGASDNSIIMWDIGGRKGRTLLLQGHHDKVQSLCYLQLTRQLVSCSSDGGIAVWNMDVSREEAPQ
  WLESDSCQKCEQPFFWNIKQMWDTKTLGLRQHHCRKCGQAVCGKCSSKRSSYPVMGFEFQVRVCDSCYDS
  IKDEDRTSLATFHEGKHNISHMSMDIARGLMVTCGTDRIVKIWDMTPVVGCSLATGFSPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020830
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020830.5
• RefSeq Size: 4607 bp
• RefSeq ORF: 1233 bp
• Locus ID: 57590
• UniProt ID: Q8IWB7
• Cytogenetics: 2q36.1
• Domains: FYVE, WD40
• MW: 46.3 kDa
• Gene Summary: The protein encoded by this gene is a phosphatidylinositol 3-phosphate binding protein, which contains a FYVE zinc finger domain and multiple WD-40 repeat domains. When exogenously expressed, it localizes to early endosomes. Mutagenesis analysis demonstrates that this endosomal localization is mediated by the FYVE domain. [provided by RefSeq, Jan 2015]