GSTO2 (NM_183239) Human Tagged ORF Clone

CAT#: RC208961

GSTO2 (Myc-DDK-tagged)-Human glutathione S-transferase omega 2 (GSTO2), transcript variant 1

Product Images

Circular map for RC208961

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GSTO2 (Cat# RC208961, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTO2(Cat# TA504083). Positive lysates LY405234 (100ug) and LC405234 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY405234) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208961 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified GSTO2 protein (Cat# TP308961). The protein was produced from HEK293T cells transfected with GSTO2 cDNA clone (Cat# RC208961) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GSTO2
• Synonyms: bA127L20.1; GSTO 2-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208961 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGGGATGCGACCAGGACCCTGGGGAAAGGAAGCCAGCCCCCAGGGCCAGTCCCGGAGGGGCTGA
  TCCGCATCTACAGCATGAGGTTCTGCCCCTATTCTCACAGGACCCGCCTCGTCCTCAAGGCCAAAGACAT
  CAGACATGAAGTGGTCAACATTAACCTGAGAAACAAGCCTGAATGGTACTATACAAAGCACCCTTTTGGC
  CACATTCCTGTCCTGGAGACCAGCCAATGTCAACTGATCTATGAATCTGTTATTGCTTGTGAGTACCTGG
  ATGATGCTTATCCAGGAAGGAAGCTGTTTCCATATGACCCTTATGAACGAGCTCGCCAAAAGATGTTATT
  GGAGCTATTTTGTAAGGTCCCACATTTGACCAAGGAGTGCCTGGTAGCGTTGAGATGTGGGAGAGAATGC
  ACTAATCTGAAGGCAGCCCTGCGTCAGGAATTCAGCAACCTGGAAGAGATTCTTGAGTATCAGAACACCA
  CCTTCTTTGGTGGAACCTGTATATCCATGATTGATTACCTCCTCTGGCCCTGGTTTGAGCGGCTGGATGT
  GTATGGGATACTGGACTGTGTGAGCCACACGCCAGCCCTGCGGCTCTGGATATCAGCCATGAAGTGGGAC
  CCCACAGTCTGTGCTCTTCTCATGGATAAGAGCATTTTCCAGGGCTTCTTGAATCTCTATTTTCAGAACA
  ACCCTAATGCCTTTGACTTTGGGCTGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208961 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGDATRTLGKGSQPPGPVPEGLIRIYSMRFCPYSHRTRLVLKAKDIRHEVVNINLRNKPEWYYTKHPFG
  HIPVLETSQCQLIYESVIACEYLDDAYPGRKLFPYDPYERARQKMLLELFCKVPHLTKECLVALRCGREC
  TNLKAALRQEFSNLEEILEYQNTTFFGGTCISMIDYLLWPWFERLDVYGILDCVSHTPALRLWISAMKWD
  PTVCALLMDKSIFQGFLNLYFQNNPNAFDFGLC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183239
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183239.2
• RefSeq Size: 1546 bp
• RefSeq ORF: 732 bp
• Locus ID: 119391
• UniProt ID: Q9H4Y5
• Cytogenetics: 10q25.1
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
• MW: 28.3 kDa
• Gene Summary: The protein encoded by this gene is an omega class glutathione S-transferase (GST). GSTs are involved in the metabolism of xenobiotics and carcinogens. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]