CTR9 (NM_014633) Human Tagged ORF Clone

CAT#: RC208933

CTR9 (Myc-DDK-tagged)-Human Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (CTR9)

Product Images

Circular map for RC208933

• 

  Western blot validation of overexpression lysate (Cat# LY415153) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208933 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CTR9
• Synonyms: p150; p150TSP; SH2BP1; TSBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208933 representing NM_014633
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGGGGCTCCATCGAGATTCCCCTCCGGGACACTGACGAGGTCATTGAACTTGACTTCGATCAGT
  TACCGGAGGGAGATGAAGTTATCAGTATTCTGAAACAGGAACACACACAACTGCACATATGGATTGCTTT
  GGCGCTGGAATACTACAAGCAAGGAAAAACAGAAGAGTTTGTAAAATTGTTGGAAGCAGCACGTATAGAT
  GGCAATTTGGACTATAGAGACCATGAAAAAGACCAGATGACTTGCTTGGATACATTGGCAGCGTATTATG
  TACAACAGGCTCGGAAAGAAAAGAATAAGGACAATAAAAAGGATCTTATTACACAGGCCACCTTGTTGTA
  TACAATGGCCGATAAAATTATTATGTATGATCAGAACCATTTGTTGGGAAGAGCCTGCTTCTGCCTACTT
  GAGGGTGACAAAATGGATCAAGCTGATGCACAGTTTCATTTTGTACTCAATCAGTCTCCAAATAATATTC
  CAGCCCTTCTTGGTAAAGCTTGCATTTCCTTCAACAAGAAGGATTACAGAGGAGCTCTTGCTTACTATAA
  GAAAGCATTGCGTACTAACCCAGGATGTCCAGCGGAAGTTCGTTTAGGAATGGGTCATTGCTTTGTGAAA
  CTTAACAAACTGGAAAAAGCTCGTCTGGCATTCAGCAGAGCCCTGGAACTCAATTCCAAATGCGTGGGAG
  CATTGGTTGGACTGGCTGTTCTAGAACTCAACAATAAAGAGGCTGATTCCATTAAAAATGGTGTCCAGCT
  TCTTTCCAGAGCCTATACTATTGATCCTAGCAACCCTATGGTATTGAACCATTTGGCAAATCACTTTTTC
  TTCAAAAAGGATTATAGTAAAGTCCAGCATCTGGCCCTCCATGCATTCCATAATACAGAAGTGGAAGCTA
  TGCAAGCAGAGAGCTGCTATCAGCTAGCTAGATCATTCCATGTTCAGGAAGATTATGACCAAGCTTTTCA
  GTACTATTATCAAGCCACACAGTTTGCCTCATCCTCTTTTGTGCTCCCATTTTTTGGTTTGGGACAAATG
  TATATTTATCGAGGTGACAAAGAAAATGCATCTCAGTGCTTTGAGAAGGTTTTGAAAGCTTATCCTAATA
  ATTACGAAACTATGAAAATTCTCGGCTCTCTCTATGCTGCCTCAGAAGATCAAGAAAAACGAGATATTGC
  CAAGGGCCATTTGAAGAAGGTCACAGAACAGTATCCCGATGATGTTGAAGCTTGGATTGAATTGGCACAA
  ATCTTAGAACAGACTGATATACAGGGTGCCCTTTCAGCCTATGGAACAGCAACACGAATCCTTCAGGAGA
  AAGTGCAGGCCGATGTTCCTCCAGAGATTCTCAATAATGTGGGTGCCCTCCATTTTAGACTTGGAAACCT
  AGGGGAGGCTAAGAAATATTTTTTGGCGTCATTGGACCGTGCAAAAGCAGAAGCGGAACATGATGAGCAT
  TACTATAACGCCATTTCCGTTACCACGTCATATAATCTCGCCAGGCTATATGAGGCGATGTGTGAATTCC
  ATGAAGCAGAAAAACTGTATAAAAACATCTTACGCGAACATCCTAATTATGTTGACTGCTATTTGCGCCT
  AGGAGCCATGGCTAGAGATAAGGGAAACTTTTATGAGGCTTCAGATTGGTTTAAGGAAGCTCTTCAGATT
  AATCAGGATCATCCAGATGCTTGGTCTTTGATTGGCAATCTTCATTTGGCAAAACAAGAATGGGGTCCTG
  GGCAGAAGAAGTTTGAGAGGATATTAAAACAGCCATCCACACAGAGTGATACCTATTCTATGCTAGCCCT
  TGGCAACGTGTGGCTCCAAACTTTACATCAGCCCACCCGAGATCGAGAAAAGGAAAAGCGTCATCAAGAT
  CGTGCTCTGGCCATCTACAAACAAGTACTCAGAAATGATGCAAAGAATCTGTATGCTGCCAATGGCATAG
  GAGCTGTTTTGGCCCACAAAGGATATTTTCGTGAAGCTCGTGATGTATTTGCCCAAGTAAGAGAAGCAAC
  AGCAGATATTAGTGATGTGTGGCTGAACTTAGCACACATCTATGTGGAGCAAAAGCAGTACATCAGCGCC
  GTTCAGATGTATGAAAACTGCCTCCGAAAGTTCTATAAGCACCAAAACACTGAAGTTGTACTCTATTTGG
  CCCGGGCCCTCTTCAAGTGTGGCAAGTTACAGGAATGCAAACAGACTTTGCTGAAGGCTAGACATGTGGC
  ACCCAGTGATACAGTTCTTATGTTTAATGTGGCCTTGGTCCTGCAAAGATTAGCTACCTCTGTCCTGAAA
  GATGAAAAAAGTAATCTGAAGGAAGTACTTAATGCTGTGAAAGAACTGGAGCTTGCACATAGATACTTCA
  GTTATTTGAGTAAAGTGGGAGATAAAATGAGATTTGATTTGGCCCTTGCTGCTACAGAAGCCAGGCAGTG
  TTCTGACTTACTGAGCCAGGCCCAGTACCATGTGGCCCGGGCACGCAAACAAGATGAAGAAGAGCGGGAG
  CTGCGGGCCAAGCAAGAGCAAGAAAAGGAGCTGTTAAGGCAGAAACTTCTTAAAGAACAGGAAGAGAAAC
  GTCTCAGAGAAAAGGAAGAGCAAAAGAAACTTTTGGAACAGCGGGCCCAGTATGTGGAGAAGACCAAAAA
  TATTCTTATGTTTACTGGTGAGACTGAAGCAACAAAAGAGAAGAAAAGAGGTGGTGGTGGTGGACGGCGT
  TCTAAGAAGGGAGGAGAGTTTGATGAATTTGTCAATGATGACACTGATGATGACCTACCTATATCCAAAA
  AGAAGAAGAGAAGAAAGGGTAGTGGCAGTGAACAAGAAGGTGAAGATGAGGAGGGTGGTGAGAGAAAGAA
  GAAAAAGAGGAGAAGACATCCAAAGGGAGAAGAAGGATCTGATGATGATGAAACAGAAAATGGCCCCAAA
  CCAAAAAAACGACGTCCACCAAAAGCAGAGAAGAAAAAGGCTCCCAAGCCAGAACGTCTGCCTCCATCAA
  TGAAGGGAAAAATAAAATCCAAAGCCATAATTTCATCAAGTGATGACTCTTCGGATGAGGATAAACTTAA
  AATTGCTGATGAAGGACATCCCAGGAACAGCAACAGCAACAGTGACTCAGACGAGGACGAACAACGAAAG
  AAATGTGCCTCATCAGAGAGTGATTCCGATGAGAACCAGAACAAGTCTGGCAGCGAGGCCGGCAGTCCCC
  GGAGGCCACGAAGACAGCGGTCAGATCAGGACTCAGACAGTGACCAGCCATCCAGAAAGAGAAGGCCCTC
  CGGTTCTGAGCAGTCTGACAATGAATCTGTGCAGTCAGGGAGAAGCCACTCAGGAGTTTCTGAGAACGAC
  TCTCGCCCAGCTTCTCCAAGTGCCGAATCAGATCACGAATCGGAGAGAGGATCTGATAATGAGGGTTCTG
  GCCAAGGCTCTGGAAATGAATCGGAACCAGAGGGATCCAACAATGAGGCCTCAGATAGAGGCTCAGAACA
  TGGGTCAGATGATAGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208933 representing NM_014633
  Red=Cloning site Green=Tags(s)
  
  MSRGSIEIPLRDTDEVIELDFDQLPEGDEVISILKQEHTQLHIWIALALEYYKQGKTEEFVKLLEAARID
  GNLDYRDHEKDQMTCLDTLAAYYVQQARKEKNKDNKKDLITQATLLYTMADKIIMYDQNHLLGRACFCLL
  EGDKMDQADAQFHFVLNQSPNNIPALLGKACISFNKKDYRGALAYYKKALRTNPGCPAEVRLGMGHCFVK
  LNKLEKARLAFSRALELNSKCVGALVGLAVLELNNKEADSIKNGVQLLSRAYTIDPSNPMVLNHLANHFF
  FKKDYSKVQHLALHAFHNTEVEAMQAESCYQLARSFHVQEDYDQAFQYYYQATQFASSSFVLPFFGLGQM
  YIYRGDKENASQCFEKVLKAYPNNYETMKILGSLYAASEDQEKRDIAKGHLKKVTEQYPDDVEAWIELAQ
  ILEQTDIQGALSAYGTATRILQEKVQADVPPEILNNVGALHFRLGNLGEAKKYFLASLDRAKAEAEHDEH
  YYNAISVTTSYNLARLYEAMCEFHEAEKLYKNILREHPNYVDCYLRLGAMARDKGNFYEASDWFKEALQI
  NQDHPDAWSLIGNLHLAKQEWGPGQKKFERILKQPSTQSDTYSMLALGNVWLQTLHQPTRDREKEKRHQD
  RALAIYKQVLRNDAKNLYAANGIGAVLAHKGYFREARDVFAQVREATADISDVWLNLAHIYVEQKQYISA
  VQMYENCLRKFYKHQNTEVVLYLARALFKCGKLQECKQTLLKARHVAPSDTVLMFNVALVLQRLATSVLK
  DEKSNLKEVLNAVKELELAHRYFSYLSKVGDKMRFDLALAATEARQCSDLLSQAQYHVARARKQDEEERE
  LRAKQEQEKELLRQKLLKEQEEKRLREKEEQKKLLEQRAQYVEKTKNILMFTGETEATKEKKRGGGGGRR
  SKKGGEFDEFVNDDTDDDLPISKKKKRRKGSGSEQEGEDEEGGERKKKKRRRHPKGEEGSDDDETENGPK
  PKKRRPPKAEKKKAPKPERLPPSMKGKIKSKAIISSSDDSSDEDKLKIADEGHPRNSNSNSDSDEDEQRK
  KCASSESDSDENQNKSGSEAGSPRRPRRQRSDQDSDSDQPSRKRRPSGSEQSDNESVQSGRSHSGVSEND
  SRPASPSAESDHESERGSDNEGSGQGSGNESEPEGSNNEASDRGSEHGSDDSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014633
• ORF Size: 3519 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_014633.3
• RefSeq Size: 4348 bp
• RefSeq ORF: 3522 bp
• Locus ID: 9646
• UniProt ID: Q6PD62
• Cytogenetics: 11p15.4
• Domains: TPR
• Protein Families: Protease
• MW: 133.3 kDa
• Gene Summary: The protein encoded by this gene is a component of the PAF1 complex, which associates with RNA polymerase II and functions in transcriptional regulation and elongation. This complex also plays a role in the modification of histones. [provided by RefSeq, Oct 2016]