ICAM3 (NM_002162) Human Tagged ORF Clone

CAT#: RC208919

ICAM3 (Myc-DDK-tagged)-Human intercellular adhesion molecule 3 (ICAM3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ICAM3 (Cat# RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3(Cat# TA507134). Positive lysates LY400788 (100ug) and LC400788 (20ug) can be purchased separately from OriGene.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM3 (RC208919, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM3 (TA507174). Positive lysates LY400788 (100ug) and LC400788 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400788) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208919 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ICAM3 protein (Cat# TP308919). The protein was produced from HEK293T cells transfected with ICAM3 cDNA clone (Cat# RC208919) using MegaTran 2.0 (Cat# TT210002).

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  Anti-ICAM3 mouse monoclonal antibody (TA507174) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ICAM3 (RC208919).

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Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ICAM3
• Synonyms: CD50; CDW50; ICAM-R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208919 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCATGGTACCATCCGTGTTGTGGCCCAGGGCCTGCTGGACTCTGCTGGTCTGCTGTCTGCTGA
  CCCCAGGTGTCCAGGGGCAGGAGTTCCTTTTGCGGGTGGAGCCCCAGAACCCTGTGCTCTCTGCTGGAGG
  GTCCCTGTTTGTGAACTGCAGTACTGATTGTCCCAGCTCTGAGAAAATCGCCTTGGAGACGTCCCTATCA
  AAGGAGCTGGTGGCCAGTGGCATGGGCTGGGCAGCCTTCAATCTCAGCAACGTGACTGGCAACAGTCGGA
  TCCTCTGCTCAGTGTACTGCAATGGCTCCCAGATAACAGGCTCCTCTAACATCACCGTGTACAGGCTCCC
  GGAGCGTGTGGAGCTGGCACCCCTGCCTCCTTGGCAGCCGGTGGGCCAGAACTTCACCCTGCGCTGCCAA
  GTGGAGGATGGGTCGCCCCGGACCAGCCTCACGGTGGTGCTGCTTCGCTGGGAGGAGGAGCTGAGCCGGC
  AGCCCGCAGTGGAGGAGCCAGCGGAGGTCACTGCCACTGTGCTGGCCAGCAGAGACGACCACGGAGCCCC
  TTTCTCATGCCGCACAGAACTGGACATGCAGCCCCAGGGGCTGGGACTGTTCGTGAACACCTCAGCCCCC
  CGCCAGCTCCGAACCTTTGTCCTGCCCGTGACCCCCCCGCGCCTCGTGGCCCCCCGGTTCTTGGAGGTGG
  AAACGTCGTGGCCGGTGGACTGCACCCTAGACGGGCTTTTTCCAGCCTCAGAGGCCCAGGTCTACCTGGC
  GCTGGGGGACCAGATGCTGAATGCGACAGTCATGAACCACGGGGACACGCTAACGGCCACAGCCACAGCC
  ACGGCGCGCGCGGATCAGGAGGGTGCCCGGGAGATCGTCTGCAACGTGACCCTAGGGGGCGAGAGACGGG
  AGGCCCGGGAGAACTTGACGGTCTTTAGCTTCCTAGGACCCATTGTGAACCTCAGCGAGCCCACCGCCCA
  TGAGGGGTCCACAGTGACCGTGAGTTGCATGGCTGGGGCTCGAGTCCAGGTCACGCTGGACGGAGTTCCG
  GCCGCGGCCCCGGGGCAGCCAGCTCAACTTCAGCTAAATGCTACCGAGAGTGACGACGGACGCAGCTTCT
  TCTGCAGTGCCACTCTCGAGGTGGACGGCGAGTTCTTGCACAGGAACAGTAGCGTCCAGCTGCGAGTCCT
  GTATGGTCCCAAAATTGACCGAGCCACATGCCCCCAGCACTTGAAATGGAAAGATAAAACGAGACACGTC
  CTGCAGTGCCAAGCCAGGGGCAACCCGTACCCCGAGCTGCGGTGTTTGAAGGAAGGCTCCAGCCGGGAGG
  TGCCGGTGGGGATCCCGTTCTTCGTCAACGTAACACATAATGGTACTTATCAGTGCCAAGCGTCCAGCTC
  ACGAGGCAAATACACCCTGGTCGTGGTGATGGACATTGAGGCTGGGAGCTCCCACTTTGTCCCCGTCTTC
  GTGGCGGTGTTACTGACCCTGGGCGTGGTGACTATCGTACTGGCCTTAATGTACGTCTTCAGGGAGCACC
  AACGGAGCGGCAGTTACCATGTTAGGGAGGAGAGCACCTATCTGCCCCTCACGTCTATGCAGCCGACAGA
  AGCAATGGGGGAAGAACCGTCCAGAGCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208919 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATMVPSVLWPRACWTLLVCCLLTPGVQGQEFLLRVEPQNPVLSAGGSLFVNCSTDCPSSEKIALETSLS
  KELVASGMGWAAFNLSNVTGNSRILCSVYCNGSQITGSSNITVYRLPERVELAPLPPWQPVGQNFTLRCQ
  VEDGSPRTSLTVVLLRWEEELSRQPAVEEPAEVTATVLASRDDHGAPFSCRTELDMQPQGLGLFVNTSAP
  RQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATA
  TARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVP
  AAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHV
  LQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSHFVPVF
  VAVLLTLGVVTIVLALMYVFREHQRSGSYHVREESTYLPLTSMQPTEAMGEEPSRAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002162
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002162.5
• RefSeq Size: 1796 bp
• RefSeq ORF: 1644 bp
• Locus ID: 3385
• UniProt ID: P32942
• Cytogenetics: 19p13.2
• Domains: ig, IG, ICAM_N
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 59.5 kDa
• Gene Summary: The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]