TCF7L1 (NM_031283) Human Tagged ORF Clone

CAT#: RC208913

TCF7L1 (Myc-DDK-tagged)-Human transcription factor 7-like 1 (T-cell specific, HMG-box) (TCF7L1)

Product Images

Circular map for RC208913

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCF7L1
• Synonyms: TCF-3; TCF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208913 representing NM_031283
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCAGCTCGGCGGCGGGGGCGGCGGCGGCGGCGGCGGCAGCGGGGGAGGCGGCGGCTCCAGCGCCG
  GGGCGGCCGGCGGAGGGGACGACCTCGGGGCGAACGACGAGCTGATCCCCTTCCAGGACGAGGGGGGCGA
  GGAGCAGGAGCCGAGCAGCGATAGCGCCTCGGCGCAGCGGGACCTAGACGAGGTCAAGTCGTCCCTGGTC
  AACGAGTCGGAGAACCAGAGCAGCAGCTCGGACTCGGAGGCGGAGAGGCGCCCGCAGCCCGTCCGGGACA
  CTTTCCAGAAGCCGCGGGACTATTTCGCCGAAGTGAGAAGGCCTCAGGACAGCGCGTTCTTTAAAGGACC
  CCCGTACCCTGGGTACCCCTTCCTGATGATCCCGGACCTGAGCAGCCCGTACCTCTCCAACGGACCCCTG
  TCTCCCGGAGGAGCGCGCACCTACCTGCAGATGAAATGGCCCCTCCTCGATGTCCCCTCCAGCGCCACAG
  TCAAGGACACGAGGTCACCATCTCCAGCACACTTGTCTAATAAAGTTCCTGTCGTTCAGCACCCGCATCA
  CATGCATCCGCTGACTCCCCTCATCACCTACAGCAATGACCACTTCTCCCCCGGCTCCCCTCCCACCCAC
  CTCTCCCCAGAGATCGATCCAAAGACAGGAATCCCCCGGCCCCCTCACCCATCCGAGCTGTCACCGTATT
  ACCCACTCTCTCCCGGAGCTGTCGGACAAATCCCCCACCCCCTCGGCTGGCTCGTCCCACAGCAAGGCCA
  GCCCATGTACTCCCTTCCTCCCGGTGGCTTCCGGCACCCTTACCCCGCCCTCGCCATGAACGCCTCGATG
  TCCAGCCTGGTCTCCAGTCGGTTCTCTCCTCACATGGTGGCTCCTGCCCACCCTGGCCTGCCCACCTCAG
  GGATCCCCCACCCTGCCATCGTCTCCCCCATCGTCAAGCAGGAACCGGCACCCCCCAGCCTGAGCCCTGC
  AGTGAGCGTGAAATCACCAGTCACCGTGAAAAAGGAGGAGGAAAAGAAGCCCCACGTGAAGAAGCCTCTG
  AATGCCTTCATGTTGTATATGAAGGAGATGAGGGCCAAGGTGGTGGCTGAGTGCACCCTGAAGGAAAGTG
  CAGCCATTAACCAGATCCTTGGAAGAAAGTGGCACAACCTGTCTCGAGAAGAACAGGCCAAGTACTACGA
  GCTGGCCCGGAAGGAGCGGCAGCTTCACTCGCAGCTCTACCCAACCTGGTCAGCCCGGGACAACTATGGT
  AAGAAAAAGAAGAGGAAGAGAGAAAAGCAGCTGTCCCAGACACAGTCACAGCAGCAAGTCCAGGAGGCAG
  AGGGTGCCCTGGCCTCCAAGAGCAAGAAGCCATGTGTTCAGTACCTGCCCCCCGAGAAGCCCTGTGACAG
  CCCTGCCTCCTCCCACGGGAGCATGCTGGACTCCCCGGCCACTCCCTCTGCAGCTTTGGCCTCACCAGCT
  GCCCCTGCTGCCACCCATTCGGAGCAAGCCCAGCCCCTCTCCCTCACCACCAAACCAGAAACCCGGGCCC
  AGCTGGCTCTCCACTCTGCCGCCTTCCTGTCGGCTAAGGCTGCAGCCTCCTCCTCTGGGCAGATGGGCAG
  CCAGCCTCCCCTCCTGTCCCGGCCCCTCCCCCTTGGGTCCATGCCCACAGCTCTGCTGGCCTCTCCCCCG
  TCCTTCCCCGCCACGCTCCATGCCCACCAGGCCCTCCCGGTGCTACAGGCCCAGCCTCTTTCCCTGGTCA
  CCAAGTCTGCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208913 representing NM_031283
  Red=Cloning site Green=Tags(s)
  
  MPQLGGGGGGGGGGSGGGGGSSAGAAGGGDDLGANDELIPFQDEGGEEQEPSSDSASAQRDLDEVKSSLV
  NESENQSSSSDSEAERRPQPVRDTFQKPRDYFAEVRRPQDSAFFKGPPYPGYPFLMIPDLSSPYLSNGPL
  SPGGARTYLQMKWPLLDVPSSATVKDTRSPSPAHLSNKVPVVQHPHHMHPLTPLITYSNDHFSPGSPPTH
  LSPEIDPKTGIPRPPHPSELSPYYPLSPGAVGQIPHPLGWLVPQQGQPMYSLPPGGFRHPYPALAMNASM
  SSLVSSRFSPHMVAPAHPGLPTSGIPHPAIVSPIVKQEPAPPSLSPAVSVKSPVTVKKEEEKKPHVKKPL
  NAFMLYMKEMRAKVVAECTLKESAAINQILGRKWHNLSREEQAKYYELARKERQLHSQLYPTWSARDNYG
  KKKKRKREKQLSQTQSQQQVQEAEGALASKSKKPCVQYLPPEKPCDSPASSHGSMLDSPATPSAALASPA
  APAATHSEQAQPLSLTTKPETRAQLALHSAAFLSAKAAASSSGQMGSQPPLLSRPLPLGSMPTALLASPP
  SFPATLHAHQALPVLQAQPLSLVTKSAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031283
• ORF Size: 1764 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031283.3
• RefSeq Size: 2932 bp
• RefSeq ORF: 1767 bp
• Locus ID: 83439
• UniProt ID: Q9HCS4
• Cytogenetics: 2p11.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway
• MW: 62.6 kDa
• Gene Summary: This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence. [provided by RefSeq, Nov 2010]