Angiogenin (ANG) (NM_001145) Human Tagged ORF Clone
ANG (Myc-DDK-tagged)-Human angiogenin, ribonuclease, RNase A family, 5 (ANG), transcript variant 1
|Product Name||Angiogenin (ANG) (NM_001145) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ALS9; HEL168; RAA1; RNASE4; RNASE5|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC208874 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC208874 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||441 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001145.1, NM_001145.2, NM_001145.3, NM_001145.4, NP_001136.1|
|RefSeq Size||1222 bp|
|RefSeq ORF||444 bp|
|Protein Families||Druggable Genome, Secreted Protein, Transmembrane|
|Gene Summary||The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]|
|The use of this cDNA Clones has been cited in the following citations:|
The cell and stress specific dynamics of in vivo and in vitro canonical and non-canonical tRNA cleavage.
,Rashad, S;Abe, T;Tominaga, T;Niizuma, K;,
Stress-induced tRNA cleavage and tiRNA generation in rat neuronal PC12 cells
,Alaa, E;Mishima, E;Niizuma, K;Akiyama, Y;Fujimura, M;Tominaga, T;Abe, T;,
J. Neurochem. 2018
,PubMed ID 29431851
|cDNA Clone Resources|
|SC119418||ANG (untagged)-Human angiogenin, ribonuclease, RNase A family, 5 (ANG), transcript variant 1||
|RG208874||ANG (GFP-tagged) - Human angiogenin, ribonuclease, RNase A family, 5 (ANG), transcript variant 1||
|RC208874L1||Lenti ORF clone of Human angiogenin, ribonuclease, RNase A family, 5 (ANG), transcript variant 1, Myc-DDK-tagged||
|RC208874L2||Lenti ORF clone of Human angiogenin, ribonuclease, RNase A family, 5 (ANG), transcript variant 1, mGFP tagged||
|RC208874L3||Lenti ORF clone of Human angiogenin, ribonuclease, RNase A family, 5 (ANG), transcript variant 1, Myc-DDK-tagged||
|RC208874L4||Lenti ORF clone of Human angiogenin, ribonuclease, RNase A family, 5 (ANG), transcript variant 1, mGFP tagged||