GDA (NM_004293) Human Tagged ORF Clone

CAT#: RC208771

GDA (Myc-DDK-tagged)-Human guanine deaminase (GDA)

Product Images

Western blot validation of overexpression lysate (Cat# LY418083) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208771 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GDA protein (Cat# TP308771). The protein was produced from HEK293T cells transfected with GDA cDNA clone (Cat# RC208771) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GDA
• Synonyms: CYPIN; GAH; GUANASE; NEDASIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208771 representing NM_004293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGCCGCTCAGATGCCGCCCCTGGCGCACATCTTCCGAGGGACGTTCGTCCACTCCACCTGGACCT
  GCCCCATGGAGGTGCTGCGGGATCACCTCCTCGGCGTGAGCGACAGCGGCAAAATAGTGTTTTTAGAAGA
  AGCATCTCAACAGGAAAAACTGGCCAAAGAATGGTGCTTCAAGCCGTGTGAAATAAGAGAACTGAGCCAC
  CATGAGTTCTTCATGCCTGGGCTGGTTGATACACACATCCATGCCTCTCAGTATTCCTTTGCTGGAAGTA
  GCATAGACCTGCCACTCTTGGAGTGGCTGACCAAGTACACATTTCCTGCAGAACACAGATTCCAGAACAT
  CGACTTTGCAGAAGAAGTATATACCAGAGTTGTCAGGAGAACACTAAAGAATGGAACAACCACAGCTTGT
  TACTTTGCAACAATTCACACTGACTCATCTCTGCTCCTTGCCGACATTACAGATAAATTTGGACAGCGGG
  CATTTGTGGGCAAAGTTTGCATGGATTTGAATGACACTTTTCCAGAATACAAGGAGACCACTGAGGAATC
  GATCAAGGAAACTGAGAGATTTGTGTCAGAAATGCTCCAAAAGAACTATTCTAGAGTGAAGCCCATAGTG
  ACACCACGTTTTTCCCTCTCCTGCTCTGAGACTTTGATGGGTGAACTGGGCAACATTGCTAAAACCCGTG
  ATTTGCACATTCAGAGCCATATAAGTGAAAATCGTGATGAAGTTGAAGCTGTGAAAAACTTATACCCCAG
  TTATAAAAACTACACATCTGTGTATGATAAAAACAATCTTTTGACAAATAAGACAGTGATGGCACACGGC
  TGCTACCTCTCTGCAGAAGAACTGAACGTATTCCATGAACGAGGAGCATCCATCGCACACTGTCCCAATT
  CTAATTTATCGCTCAGCAGTGGATTTCTAAATGTGCTAGAAGTCCTGAAACATGAAGTCAAGATAGGGCT
  GGGTACAGACGTGGCTGGTGGCTATTCATATTCCATGCTTGATGCAATCAGAAGAGCAGTGATGGTTTCC
  AATATCCTTTTAATTAATAAGGTAAATGAGAAAAGCCTCACCCTCAAAGAAGTCTTCAGACTAGCTACTC
  TTGGAGGAAGCCAAGCCCTGGGGCTGGATGGTGAGATTGGAAACTTTGAAGTGGGCAAGGAATTTGATGC
  CATCCTGATCAACCCCAAAGCATCCGACTCTCCCATTGACCTGTTTTATGGGGACTTTTTTGGTGATATT
  TCTGAGGCTGTTATCCAGAAGTTCCTCTATCTAGGAGATGATCGAAATATTGAAGAGGTTTATGTGGGCG
  GAAAGCAGGTGGTTCCGTTTTCCAGCTCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208771 representing NM_004293
  Red=Cloning site Green=Tags(s)
  
  MCAAQMPPLAHIFRGTFVHSTWTCPMEVLRDHLLGVSDSGKIVFLEEASQQEKLAKEWCFKPCEIRELSH
  HEFFMPGLVDTHIHASQYSFAGSSIDLPLLEWLTKYTFPAEHRFQNIDFAEEVYTRVVRRTLKNGTTTAC
  YFATIHTDSSLLLADITDKFGQRAFVGKVCMDLNDTFPEYKETTEESIKETERFVSEMLQKNYSRVKPIV
  TPRFSLSCSETLMGELGNIAKTRDLHIQSHISENRDEVEAVKNLYPSYKNYTSVYDKNNLLTNKTVMAHG
  CYLSAEELNVFHERGASIAHCPNSNLSLSSGFLNVLEVLKHEVKIGLGTDVAGGYSYSMLDAIRRAVMVS
  NILLINKVNEKSLTLKEVFRLATLGGSQALGLDGEIGNFEVGKEFDAILINPKASDSPIDLFYGDFFGDI
  SEAVIQKFLYLGDDRNIEEVYVGGKQVVPFSSSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004293
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004293.5
• RefSeq Size: 5430 bp
• RefSeq ORF: 1365 bp
• Locus ID: 9615
• UniProt ID: Q9Y2T3
• Cytogenetics: 9q21.13
• Domains: Amidohydro_1
• Protein Pathways: Metabolic pathways, Purine metabolism
• MW: 50.8 kDa
• Gene Summary: This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]