RbAp48 (RBBP4) (NM_005610) Human Tagged ORF Clone

CAT#: RC208761

RBBP4 (Myc-DDK-tagged)-Human retinoblastoma binding protein 4 (RBBP4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417190) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208761 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RBBP4 protein (Cat# TP308761). The protein was produced from HEK293T cells transfected with RBBP4 cDNA clone (Cat# RC208761) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RbAp48
• Synonyms: lin-53; NURF55; RBAP48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208761 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACAAGGAAGCAGCCTTCGACGACGCAGTGGAAGAACGAGTGATCAACGAGGAATACAAAATAT
  GGAAAAAGAACACCCCTTTTCTTTATGATTTGGTGATGACCCATGCTCTGGAGTGGCCCAGCCTAACTGC
  CCAGTGGCTTCCAGATGTAACCAGACCAGAAGGGAAAGATTTCAGCATTCATCGACTTGTCCTGGGGACA
  CACACATCGGATGAACAAAACCATCTTGTTATAGCCAGTGTGCAGCTCCCTAATGATGATGCTCAGTTTG
  ATGCGTCACACTACGACAGTGAGAAAGGAGAATTTGGAGGTTTTGGTTCAGTTAGTGGAAAAATTGAAAT
  AGAAATCAAGATCAACCATGAAGGAGAAGTAAACAGGGCCCGTTATATGCCCCAGAACCCTTGTATCATC
  GCAACAAAGACTCCTTCCAGTGATGTTCTTGTCTTTGACTATACAAAACATCCTTCTAAACCAGATCCTT
  CTGGAGAGTGCAACCCAGACTTGCGTCTCCGTGGACATCAGAAGGAAGGCTATGGGCTTTCTTGGAACCC
  AAATCTCAGTGGGCACTTACTTAGTGCTTCAGATGACCATACCATCTGCCTGTGGGACATCAGTGCCGTT
  CCAAAGGAGGGAAAAGTGGTAGATGCGAAGACCATCTTTACAGGGCATACGGCAGTAGTAGAAGATGTTT
  CCTGGCATCTACTCCATGAGTCTCTGTTTGGGTCAGTTGCTGATGATCAGAAACTTATGATTTGGGATAC
  TCGTTCAAACAATACTTCCAAACCAAGCCACTCAGTTGATGCTCACACTGCTGAAGTGAACTGCCTTTCT
  TTCAATCCTTATAGTGAGTTCATTCTTGCCACAGGATCAGCTGACAAGACTGTTGCCTTGTGGGATCTGA
  GAAATCTGAAACTTAAGTTGCATTCCTTTGAGTCACATAAGGATGAAATATTCCAGGTTCAGTGGTCACC
  TCACAATGAGACTATTTTAGCTTCCAGTGGTACTGATCGCAGACTGAATGTCTGGGATTTAAGTAAAATT
  GGAGAGGAACAATCCCCAGAAGATGCAGAAGACGGGCCACCAGAGTTGTTGTTTATTCATGGTGGTCATA
  CTGCCAAGATATCTGATTTCTCCTGGAATCCCAATGAACCTTGGGTGATTTGTTCTGTATCAGAAGACAA
  TATCATGCAAGTGTGGCAAATGGCAGAGAACATTTATAATGATGAAGACCCTGAAGGAAGCGTGGATCCA
  GAAGGACAAGGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208761 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGT
  HTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCII
  ATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAV
  PKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLS
  FNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKI
  GEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDP
  EGQGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005610
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005610.1, NP_005601.1
• RefSeq Size: 7943 bp
• RefSeq ORF: 1278 bp
• Locus ID: 5928
• UniProt ID: Q09028
• Cytogenetics: 1p35.1
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• MW: 47.7 kDa
• Gene Summary: This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]