ARHGEF11 (NM_198236) Human Tagged ORF Clone

CAT#: RC208729

ARHGEF11 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 11 (ARHGEF11), transcript variant 2

Product Images

Circular map for RC208729

• 

  Western blot validation of overexpression lysate (Cat# LY404959) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208729 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ARHGEF11 protein (Cat# TP308729). The protein was produced from HEK293T cells transfected with ARHGEF11 cDNA clone (Cat# RC208729) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGEF11
• Synonyms: GTRAP48; PDZ-RHOGEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208729 representing NM_198236
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTAAGGTTACCCCAGAGTATAGACAGGTTAAGTAGCCTGTCTTCTCTGGGAGATTCTGCACCAG
  AGCGCAAGTCCCCTTCCCACCATCGCCAGCCTTCGGATGCCTCTGAGACAACAGGTCTCGTTCAACGCTG
  TGTCATTATCCAAAAGGACCAGCATGGCTTCGGCTTCACAGTCAGTGGGGATCGCATTGTTCTGGTGCAG
  TCTGTGCGGCCTGGAGGTGCAGCCATGAAGGCCGGTGTGAAAGAGGGCGACCGGATCATCAAAGTCAACG
  GCACCATGGTGACCAATAGCTCACACCTGGAAGTGGTAAAGCTGATCAAATCTGGCGCCTATGTCGCACT
  CACCCTCCTGGGCTCTTCACCTTCATCCATGGGCATCTCTGGGCTCCAGCAGGACCCATCCCCAGCAGGA
  GCTCCCCGAATCACGTCAGTGATCCCCTCACCACCACCTCCTCCACCTCTACCACCTCCACAACGCATCA
  CAGGACCCAAACCTCTGCAGGATCCCGAAGTTCAAAAACATGCCACCCAGATCCTCAGGAATATGCTGAG
  GCAGGAAGAAAAAGAATTACAGCGTATCTGTGAGGTCTATAGCCGGAACCCCGCCAGCCTACTGGAAGAG
  CAGATCGAAGGTGCCCGGCGGCGAGTCACTCAGTTACAGCTGAAGATCCAGCAGGAGACTGGTGGCTCAG
  TGGACATACTTCCACTATATGGTGACACCAGCCAGAGACCATCAGAAGGCCGGCTCTCTCTGGATTCCCA
  GGAGGGGGACAGTGGCTTGGACTCTGGGACAGAACGCTTTCCTTCCCTCAGTGAGTCATTGATGAATCGG
  AACTCGGTACTGTCAGACCCTGGGCTAGACAGTCCTCGAACCTCCCCTGTGATCATGGCCAGGGTGGCCC
  AGCACCACAGGCGGCAGGGCTCGGATGCAGCAGTCCCCTCAACCGGTGACCAGGGTGTAGATCAAAGCCC
  AAAGCCTTTAATTATTGGCCCAGAGGAAGACTATGACCCGGGTTATTTCAACAACGAGAGCGACATCATA
  TTCCAGGATCTGGAGAAACTGAAGTCTCGGCCAGCTCACCTGGGGGTTTTTCTACGTTACATCTTCTCTC
  AGGCGGACCCCAGTCCACTGCTTTTTTACCTGTGTGCAGAAGTTTATCAGCAGGCAAGCCCCAAGGATTC
  CCGAAGCTTGGGGAAAGACATCTGGAATATTTTCCTGGAGAAAAATGCGCCTCTGAGAGTGAAGATCCCT
  GAGATGCTACAGGCTGAAATTGACTCGCGCCTGCGGAACAGCGAAGATGCCCGTGGTGTTCTCTGTGAAG
  CTCAAGAGGCAGCCATGCCTGAGATCCAAGAGCAGATCCACGACTACAGAACGAAGCGCACACTGGGGCT
  GGGCAGCCTGTATGGTGAAAATGACCTGCTGGACCTGGATGGGGACCCTCTCCGAGAGCGCCAAGTGGCT
  GAGAAGCAGCTGGCTGCCCTTGGAGATATTTTGTCCAAGTATGAGGAAGACAGGAGCGCCCCCATGGACT
  TCGCCCTCAATACCTACATGAGCCATGCTGGGATCCGTCTTCGAGAGGCACGACCTTCCAACACAGCTGA
  AAAGGCCCAGTCTGCTCCTGACAAGGACAAGTGGCTACCGTTCTTCCCTAAGACCAAGAAGAGCAGCAAT
  TCCAAGAAAGAAAAGGATGCCTTGGAGGACAAGAAGCGAAACCCTATCCTCAAATACATTGGGAAGCCCA
  AAAGCTCTTCTCAAAGCACATTTCATATTCCCTTGTCCCCTGTGGAAGTCAAACCAGGCAATGTGAGGAA
  CATCATTCAGCACTTTGAGAACAACCAGCAGTATGATGCCCCAGAACCTGGGACACAACGACTCTCGACC
  GGAAGCTTTCCTGAGGACCTGCTGGAGAGTGACAGTTCACGCTCAGAGATTCGCCTGGGCCGCTCTGAAA
  GCCTCAAGGGCCGGGAAGAGATGAAACGGTCTCGAAAGGCAGAGAACGTGCCCCGCTCTCGCAGTGATGT
  TGACATGGATGCTGCTGCGGAGGCTACTCGCCTGCACCAGTCAGCCTCGTCCTCTACCTCCAGCCTCTCC
  ACCAGGTCTCTTGAGAACCCAACCCCTCCATTCACTCCCAAAATGGGCCGCAGGAGCATTGAGTCCCCCA
  GTTTGGGGTTCTGCACAGATACCCTCCTTCCCCACCTCCTAGAGGATGATCTGGGCCAGCTGTCTGACCT
  GGAGCCAGAGCCAGATGCCCAAAATTGGCAGCATACAGTGGGCAAGGATGTGGTGGCTGGGCTAACCCAG
  CGGGAGATTGACCGGCAAGAGGTCATCAATGAGCTGTTTGTGACTGAAGCTTCCCACCTGCGCACACTCC
  GGGTCCTGGACCTGATCTTCTACCAGCGAATGAAGAAGGAGAACCTGATGCCCCGGGAGGAGCTGGCCCG
  GCTCTTCCCGAACCTGCCTGAACTCATAGAGATTCACAATTCCTGGTGTGAAGCCATGAAGAAGCTCCGG
  GAGGAAGGCCCCATCATCAAAGAGATCAGTGACCTCATGCTGGCCCGGTTTGATGGCCCTGCCCGAGAGG
  AACTCCAGCAAGTGGCTGCACAGTTCTGTTCCTATCAGTCAATAGCCCTAGAGCTAATCAAGACCAAGCA
  ACGCAAGGAGAGTCGATTCCAGCTCTTCATGCAGGAGGCTGAGAGCCACCCTCAGTGTCGGCGGCTGCAG
  CTGAGAGACCTCATCATCTCTGAGATGCAGCGGCTCACCAAGTACCCGCTGCTGCTGGAGAGCATCATCA
  AGCACACAGAGGGTGGCACCTCTGAGCATGAGAAGCTGTGCCGGGCCCGGGACCAGTGCCGGGAGATTCT
  CAAGTATGTGAATGAAGCGGTAAAACAAACAGAGAACCGCCACCGTTTAGAGGGCTACCAGAAACGCCTG
  GATGCCACCGCCCTGGAGAGGGCCAGCAACCCCCTGGCAGCAGAGTTCAAGAGCCTGGATCTTACAACCA
  GAAAAATGATCCATGAGGGACCCCTGACCTGGAGGATCAGCAAGGATAAGACCTTGGACCTCCACGTGCT
  GCTGCTGGAGGACCTCCTAGTGCTGCTACAGAAACAGGATGAGAAGCTATTGCTGAAGTGCCACAGCAAG
  ACTGCTGTGGGCTCCTCAGACAGCAAGCAGACCTTCAGCCCCGTGCTCAAGCTCAATGCTGTGCTCATCC
  GCTCTGTGGCCACAGATAAACGGGCCTTCTTCATCATCTGCACCTCCAAGCTGGGCCCACCCCAGATCTA
  TGAGCTGGTTGCATTGACGTCATCAGACAAGAACACATGGATGGAGCTCTTAGAAGAGGCCGTGCGGAAT
  GCCACCAGGCACCCCGGAGCTGCCCCAATGCCCGTCCATCCTCCACCCCCAGGTCCCCGGGAGCCAGCCC
  AGCAGGGCCCCACACCCAGCAGGGTAGAACTGGATGACTCAGACGTGTTCCATGGTGAACCTGAACCTGA
  GGAGCTGCCTGGAGGCACTGGGTCCCAGCAGAGGGTCCAAGGGAAGCACCAGGTCCTGCTAGAGGACCCT
  GAGCAGGAGGGCAGTGCAGAGGAAGAGGAACTGGGTGTCCTGCCTTGCCCTTCCACATCCCTGGATGGAG
  AGAACAGGGGCATCAGGACAAGGAACCCCATCCACTTGGCCTTCCCAGGCCCTCTGTTCATGGAAGGGCT
  CGCTGACTCCGCTCTGGAAGATGTGGAGAACCTGCGACATCTGATCCTGTGGAGCCTGCTGCCAGGTCAC
  ACCATGGAAACTCAGGCTGCCCAGGAGCCCGAGGACGACCTGACACCCACACCTTCTGTCATCAGCGTCA
  CCTCTCACCCCTGGGACCCAGGCTCCCCAGGGCAAGCACCCCCTGGGGGTGAAGGGGACAACACCCAGCT
  TGCAGGGCTGGAGGGGGAACGGCCAGAGCAGGAAGACATGGGTCTCTGTTCTCTGGAACACCTACCCCCA
  AGGACCAGAAATTCTGGGATATGGGAGTCTCCAGAACTGGACAGGAATCTGGCTGAAGATGCTTCAAGCA
  CAGAGGCAGCAGGAGGTTACAAAGTTGTGAGAAAAGCTGAGGTGGCAGGCAGCAAGGTTGTCCCTGCACT
  ACCAGAGAGTGGCCAGTCAGAGCCTGGGCCACCTGAAGTGGAAGGCGGAACAAAGGCTACGGGGAACTGC
  TTTTATGTCAGCATGCCATCAGGACCCCCGGACTCAAGCACCGACCACTCAGAGGCACCCATGAGCCCCC
  CTCAGCCTGACAGCCTCCCTGCAGGGCAGACAGAGCCTCAGCCTCAGCTGCAGGGAGGCAACGATGATCC
  AAGACGCCCCAGCCGCTCTCCTCCAAGCCTGGCCCTCAGGGACGTGGGCATGATCTTCCATACCATTGAG
  CAGCTCACTCTCAAGCTCAACAGGCTCAAGGATATGGAGCTGGCCCACAGAGAGCTGCTCAAGTCCCTTG
  GGGGAGAGTCATCTGGTGGCACCACGCCTGTGGGCAGTTTCCACACAGAAGCAGCTAGATGGACAGATGG
  CTCCCTCTCACCTCCCGCTAAGGAGCCCCTAGCTTCTGACTCCAGGAACAGCCATGAACTGGGGCCCTGC
  CCTGAGGATGGCTCTGACGCCCCCCTGGAAGACAGCACAGCAGACGCAGCCGCGTCACCAGGACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208729 representing NM_198236
  Red=Cloning site Green=Tags(s)
  
  MSVRLPQSIDRLSSLSSLGDSAPERKSPSHHRQPSDASETTGLVQRCVIIQKDQHGFGFTVSGDRIVLVQ
  SVRPGGAAMKAGVKEGDRIIKVNGTMVTNSSHLEVVKLIKSGAYVALTLLGSSPSSMGISGLQQDPSPAG
  APRITSVIPSPPPPPPLPPPQRITGPKPLQDPEVQKHATQILRNMLRQEEKELQRICEVYSRNPASLLEE
  QIEGARRRVTQLQLKIQQETGGSVDILPLYGDTSQRPSEGRLSLDSQEGDSGLDSGTERFPSLSESLMNR
  NSVLSDPGLDSPRTSPVIMARVAQHHRRQGSDAAVPSTGDQGVDQSPKPLIIGPEEDYDPGYFNNESDII
  FQDLEKLKSRPAHLGVFLRYIFSQADPSPLLFYLCAEVYQQASPKDSRSLGKDIWNIFLEKNAPLRVKIP
  EMLQAEIDSRLRNSEDARGVLCEAQEAAMPEIQEQIHDYRTKRTLGLGSLYGENDLLDLDGDPLRERQVA
  EKQLAALGDILSKYEEDRSAPMDFALNTYMSHAGIRLREARPSNTAEKAQSAPDKDKWLPFFPKTKKSSN
  SKKEKDALEDKKRNPILKYIGKPKSSSQSTFHIPLSPVEVKPGNVRNIIQHFENNQQYDAPEPGTQRLST
  GSFPEDLLESDSSRSEIRLGRSESLKGREEMKRSRKAENVPRSRSDVDMDAAAEATRLHQSASSSTSSLS
  TRSLENPTPPFTPKMGRRSIESPSLGFCTDTLLPHLLEDDLGQLSDLEPEPDAQNWQHTVGKDVVAGLTQ
  REIDRQEVINELFVTEASHLRTLRVLDLIFYQRMKKENLMPREELARLFPNLPELIEIHNSWCEAMKKLR
  EEGPIIKEISDLMLARFDGPAREELQQVAAQFCSYQSIALELIKTKQRKESRFQLFMQEAESHPQCRRLQ
  LRDLIISEMQRLTKYPLLLESIIKHTEGGTSEHEKLCRARDQCREILKYVNEAVKQTENRHRLEGYQKRL
  DATALERASNPLAAEFKSLDLTTRKMIHEGPLTWRISKDKTLDLHVLLLEDLLVLLQKQDEKLLLKCHSK
  TAVGSSDSKQTFSPVLKLNAVLIRSVATDKRAFFIICTSKLGPPQIYELVALTSSDKNTWMELLEEAVRN
  ATRHPGAAPMPVHPPPPGPREPAQQGPTPSRVELDDSDVFHGEPEPEELPGGTGSQQRVQGKHQVLLEDP
  EQEGSAEEEELGVLPCPSTSLDGENRGIRTRNPIHLAFPGPLFMEGLADSALEDVENLRHLILWSLLPGH
  TMETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDMGLCSLEHLPP
  RTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPESGQSEPGPPEVEGGTKATGNC
  FYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQLQGGNDDPRRPSRSPPSLALRDVGMIFHTIE
  QLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPC
  PEDGSDAPLEDSTADAAASPGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198236
• ORF Size: 4686 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198236.3
• RefSeq Size: 6904 bp
• RefSeq ORF: 4689 bp
• Locus ID: 9826
• UniProt ID: O15085
• Cytogenetics: 1q23.1
• Protein Families: Druggable Genome
• Protein Pathways: Vascular smooth muscle contraction
• MW: 172.1 kDa
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]