CASZ1 (NM_017766) Human Tagged ORF Clone

CAT#: RC208715

CASZ1 (Myc-DDK-tagged)-Human castor zinc finger 1 (CASZ1), transcript variant 2

Product Images

Circular map for RC208715

• 

  Western blot validation of overexpression lysate (Cat# LY413566) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208715 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CASZ1
• Synonyms: CAS11; CST; dJ734G22.1; SRG; ZNF693
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208715 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTTGGAACAGCTGAGGGCACCCGGTGCACGGACCCGCCTGCAGGCAAGCCCGCCATGGCGCCCA
  AACGCAAGGGTGGCCTGAAGCTGAACGCCATCTGCGCCAAGCTGAGCCGCCAGGTGGTGGTGGAGAAGCG
  AGCTGACGCCGGCTCCCACACGGAGGGCAGCCCATCGCAGCCCCGGGACCAAGAGCGCAGTGGCCCTGAG
  TCTGGGGCAGCCCGGGCCCCCCGCAGCGAGGAAGACAAGAGACGGGCAGTGATCGAGAAGTGGGTGAACG
  GGGAGTACAGCGAGGAGCCGGCACCCACACCCGTGTTGGGGCGGATTGCCCGCGAGGGCCTGGAGCTGCC
  TCCCGAGGGTGTCTACATGGTGCAGCCCCAGGGGTGCAGCGATGAGGAAGACCACGCGGAGGAGCCCTCC
  AAGGACGGCGGTGCCCTGGAGGAGAAGGATTCGGACGGGGCAGCCTCCAAGGAGGACAGCGGCCCCAGCA
  CCAGGCAGGCTTCAGGAGAGGCCTCCTCGCTGCGGGACTACGCGGCCTCCACCATGACCGAGTTCCTCGG
  CATGTTTGGCTATGATGACCAGAACACGCGGGACGAGCTGGCCAGGAAGATCAGCTTTGAGAAGCTGCAC
  GCGGGCTCCACCCCGGAGGCAGCCACCTCCTCCATGCTGCCCACCTCCGAGGATACCCTCAGCAAGCGGG
  CGCGGTTCTCTAAGTATGAGGAGTACATCCGCAAGCTCAAGGCTGGCGAGCAGCTCTCCTGGCCGGCCCC
  CAGCACCAAGACCGAGGAGCGGGTGGGCAAGGAGGTGGTGGGCACCCTGCCCGGCCTGCGGCTGCCCAGC
  AGCACGGCCCACCTGGAGACCAAGGCCACCATCCTGCCCCTGCCGTCGCACAGCAGTGTCCAGATGCAGA
  ACCTGGTAGCCCGGGCCTCCAAGTACGACTTCTTCATCCAAAAACTGAAGACCGGCGAGAATCTGCGGCC
  CCAGAACGGGAGCACCTACAAGAAGCCATCCAAGTACGACCTGGAGAATGTCAAGTACCTGCACCTCTTC
  AAACCCGGGGAGGGCAGCCCCGACATGGGCGGGGCCATCGCCTTCAAGACAGGCAAGGTGGGGCGCCCTT
  CCAAGTACGACGTCCGGGGCATCCAGAAGCCAGGCCCCGCCAAGGTTCCGCCCACCCCCAGCCTGGCTCC
  CGCACCCCTCGCCAGCGTGCCCAGTGCCCCCAGCGCCCCCGGGCCAGGGCCAGAGCCTCCTGCCTCCCTG
  TCCTTCAACACTCCCGAGTACCTGAAGTCAACCTTCTCCAAAACAGACTCCATCACCACGGGGACCGTCT
  CCACTGTCAAGAACGGACTGCCCACAGATAAACCAGCCGTCACTGAAGATGTAAACATTTACCAGAAATA
  TATTGCCAGGTTCTCGGGCAGCCAGCACTGTGGCCACATCCACTGTGCCTACCAGTACCGCGAGCACTAC
  CACTGCCTTGACCCTGAGTGTAACTACCAGAGGTTCACGAGTAAGCAGGACGTGATCCGCCACTACAACA
  TGCACAAGAAGCGCGACAACTCCCTGCAGCACGGCTTCATGCGTTTCAGCCCGCTGGACGACTGCAGCGT
  CTACTACCACGGCTGCCACCTCAATGGGAAGAGCACCCACTATCACTGCATGCAGGTGGGCTGTAACAAG
  GTGTACACGAGCACGTCTGACGTGATGACCCACGAGAACTTCCACAAGAAGAATACCCAGCTCATTAACG
  ACGGCTTCCAGCGCTTCCGAGCCACCGAAGACTGTGGCACAGCCGACTGCCAGTTCTACGGACAGAAGAC
  CACGCACTTCCACTGCAGGCGCCCCGGCTGCACATTCACTTTCAAGAACAAGTGTGACATCGAGAAGCAC
  AAGAGCTACCACATCAAGGACGATGCCTACGCCAAGGACGGCTTCAAGAAGTTCTACAAGTACGAGGAGT
  GCAAGTACGAGGGCTGCGTGTACAGCAAGGCTACCAACCACTTCCACTGCATCCGCGCCGGCTGCGGCTT
  CACCTTCACCTCCACCAGCCAGATGACCTCTCACAAGCGCAAGCATGAGCGCCGGCACATCCGCTCCTCG
  GGCGCGCTGGGGCTGCCGCCCTCGCTGCTGGGCGCCAAGGACACGGAGCACGAGGAGTCCAGCAACGACG
  ACCTTGTTGACTTCTCCGCCCTGAGCAGCAAGAACTCCAGCCTGAGCGCCTCCCCTACCAGCCAGCAGTC
  CTCTGCGTCCCTGGCTGCCGCCACTGCCGCCACCGAGGCTGGGCCCAGTGCCACCAAACCTCCCAACAGC
  AAGATCTCGGGGCTGCTGCCCCAGGGCCTGCCTGGCTCGATCCCCCTGGCCCTGGCCCTCTCCAACTCGG
  GCCTGCCCACCCCCACGCCCTACTTCCCCATACTGGCTGGCCGTGGGAGCACCTCCCCGCCTGTGGGCAC
  CCCCAGCCTCCTGGGTGCCGTGTCGTCTGGGTCAGCAGCCTCAGCCACCCCTGACACACCCACGCTGGTC
  GCCTCGGGAGCTGGAGACTCAGCCCCCGTGGCTGCCGCCTCTGTCCCGGCACCACCCGCCTCCATCATGG
  AGAGGATCTCTGCAAGCAAGGGCCTCATCTCGCCCATGATGGCCAGGCTGGCTGCAGCTGCCCTTAAGCC
  CTCTGCCACCTTTGACCCAGGAAGCGGGCAGCAGGTCACCCCAGCCAGGTTCCCCCCGGCCCAAGTGAAG
  CCGGAACCCGGTGAGAGCACCGGCGCCCCAGGCCCCCACGAAGCCTCCCAGGACCGCAGTCTAGACCTGA
  CTGTGAAGGAGCCCAGCAACGAATCAAATGGCCACGCAGTCCCGGCAAATTCATCTCTTTTATCCTCGCT
  TATGAATAAGATGTCTCAGGGCAACCCTGGCCTGGGCAGCCTGCTGAACATCAAGGCGGAAGCGGAGGGG
  AGCCCCGCTGCGGAGCCCTCGCCCTTCCTAGGCAAGGCCGTGAAGGCGCTGGTTCAGGAGAAGTTGGCAG
  AGCCCTGGAAGGTGTACCTGCGCAGGTTTGGTACAAAGGACTTCTGTGACGGCCAGTGTGACTTCCTCCA
  CAAGGCCCACTTCCACTGCGTGGTGGAGGAATGCGGCGCGCTCTTCAGCACCTTGGACGGGGCCATCAAG
  CACGCAAACTTCCACTTCCGGACAGAGGGAGGAGCAGCAAAAGGAAACACAGAGGCTGCCTTTCCGGCCT
  CGGCCGCCGAGACCAAACCTCCCATGGCCCCCTCGTCCCCTCCGGTCCCTCCTGTCACCACGGCCACGGT
  GTCCTCTCTGGAGGGGCCCGCTCCCAGCCCGGCCTCCGTGCCCTCCACCCCCACCCTGCTCGCCTGGAAG
  CAGCTGGCTTCCACCATACCCCAGATGCCTCAGATCCCAGCGTCAGTGCCTCACCTGCCCGCCTCGCCCT
  TGGCAACGACTTCTCTAGAGAACGCCAAGCCCCAGGTCAAACCCGGATTCCTCCAGTTCCAGGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208715 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLGTAEGTRCTDPPAGKPAMAPKRKGGLKLNAICAKLSRQVVVEKRADAGSHTEGSPSQPRDQERSGPE
  SGAARAPRSEEDKRRAVIEKWVNGEYSEEPAPTPVLGRIAREGLELPPEGVYMVQPQGCSDEEDHAEEPS
  KDGGALEEKDSDGAASKEDSGPSTRQASGEASSLRDYAASTMTEFLGMFGYDDQNTRDELARKISFEKLH
  AGSTPEAATSSMLPTSEDTLSKRARFSKYEEYIRKLKAGEQLSWPAPSTKTEERVGKEVVGTLPGLRLPS
  STAHLETKATILPLPSHSSVQMQNLVARASKYDFFIQKLKTGENLRPQNGSTYKKPSKYDLENVKYLHLF
  KPGEGSPDMGGAIAFKTGKVGRPSKYDVRGIQKPGPAKVPPTPSLAPAPLASVPSAPSAPGPGPEPPASL
  SFNTPEYLKSTFSKTDSITTGTVSTVKNGLPTDKPAVTEDVNIYQKYIARFSGSQHCGHIHCAYQYREHY
  HCLDPECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCNK
  VYTSTSDVMTHENFHKKNTQLINDGFQRFRATEDCGTADCQFYGQKTTHFHCRRPGCTFTFKNKCDIEKH
  KSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRAGCGFTFTSTSQMTSHKRKHERRHIRSS
  GALGLPPSLLGAKDTEHEESSNDDLVDFSALSSKNSSLSASPTSQQSSASLAAATAATEAGPSATKPPNS
  KISGLLPQGLPGSIPLALALSNSGLPTPTPYFPILAGRGSTSPPVGTPSLLGAVSSGSAASATPDTPTLV
  ASGAGDSAPVAAASVPAPPASIMERISASKGLISPMMARLAAAALKPSATFDPGSGQQVTPARFPPAQVK
  PEPGESTGAPGPHEASQDRSLDLTVKEPSNESNGHAVPANSSLLSSLMNKMSQGNPGLGSLLNIKAEAEG
  SPAAEPSPFLGKAVKALVQEKLAEPWKVYLRRFGTKDFCDGQCDFLHKAHFHCVVEECGALFSTLDGAIK
  HANFHFRTEGGAAKGNTEAAFPASAAETKPPMAPSSPPVPPVTTATVSSLEGPAPSPASVPSTPTLLAWK
  QLASTIPQMPQIPASVPHLPASPLATTSLENAKPQVKPGFLQFQEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017766
• ORF Size: 3498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017766.5
• RefSeq Size: 4438 bp
• RefSeq ORF: 3501 bp
• Locus ID: 54897
• UniProt ID: Q86V15
• Cytogenetics: 1p36.22
• MW: 124.8 kDa
• Gene Summary: The protein encoded by this gene is a zinc finger transcription factor. The encoded protein may function as a tumor suppressor, and single nucleotide polymorphisms in this gene are associated with blood pressure variation. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]