MYG1 (NM_021640) Human Tagged ORF Clone

CAT#: RC208702

C12orf10 (Myc-DDK-tagged)-Human chromosome 12 open reading frame 10 (C12orf10)

Product Images

Circular map for RC208702

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  Western blot validation of overexpression lysate (Cat# LY411950) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208702 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified C12orf10 protein (Cat# TP308702). The protein was produced from HEK293T cells transfected with C12orf10 cDNA clone (Cat# RC208702) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYG1
• Synonyms: C12orf10; Gamm1; MST024; MSTP024; MYG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208702 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACACCGCTTCCTGCGCGGCCTCTTAACGCTGCTGCTGCCGCCGCCACCCCTGTATACCCGGCACC
  GCATGCTCGGTCCAGAGTCCGTCCCGCCCCCAAAACGATCCCGCAGCAAACTCATGGCACCGCCCCGAAT
  CGGGACGCACAATGGCACCTTCCACTGCGACGAGGCACTGGCATGCGCACTGCTTCGCCTCCTGCCGGAG
  TACCGGGATGCAGAGATTGTGCGGACCCGGGATCCCGAAAAACTCGCTTCCTGTGACATCGTGGTGGACG
  TGGGGGGCGAGTACGACCCTCGGAGACACCGATATGACCATCACCAGAGGTCTTTCACAGAGACCATGAG
  CTCCCTGTCCCCTGGGAAGCCGTGGCAGACCAAGCTGAGCAGTGCGGGACTCATCTATCTGCACTTCGGG
  CACAAGCTGCTGGCCCAGTTGCTGGGCACTAGTGAAGAGGACAGCATGGTGGGCACCCTCTATGACAAGA
  TGTATGAGAACTTTGTGGAGGAGGTGGATGCTGTGGACAATGGGATCTCCCAGTGGGCAGAGGGGGAGCC
  TCGATATGCACTGACCACTACCCTGAGTGCACGAGTTGCTCGACTTAATCCTACCTGGAACCACCCCGAC
  CAAGACACTGAGGCAGGGTTCAAGCGTGCAATGGATCTGGTTCAAGAGGAGTTTCTGCAGAGATTAGATT
  TCTACCAACACAGCTGGCTGCCAGCCCGGGCCTTGGTGGAAGAGGCCCTTGCCCAGCGATTCCAGGTGGA
  CCCAAGTGGAGAGATTGTGGAACTGGCGAAAGGTGCATGTCCCTGGAAGGAGCATCTCTACCACCTGGAA
  TCTGGGCTGTCCCCTCCAGTGGCCATCTTCTTTGTTATCTACACTGACCAGGCTGGACAGTGGCGAATAC
  AGTGTGTGCCCAAGGAGCCCCACTCATTCCAAAGCCGGCTGCCCCTGCCAGAGCCATGGCGGGGTCTTCG
  GGACGAGGCCCTGGACCAGGTCAGTGGGATCCCTGGCTGCATCTTCGTCCATGCAAGCGGCTTCATTGGC
  GGTCACCGCACCCGAGAGGGTGCCTTGAGCATGGCCCGTGCCACCTTGGCCCAGCGCTCATACCTCCCAC
  AAATCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208702 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGHRFLRGLLTLLLPPPPLYTRHRMLGPESVPPPKRSRSKLMAPPRIGTHNGTFHCDEALACALLRLLPE
  YRDAEIVRTRDPEKLASCDIVVDVGGEYDPRRHRYDHHQRSFTETMSSLSPGKPWQTKLSSAGLIYLHFG
  HKLLAQLLGTSEEDSMVGTLYDKMYENFVEEVDAVDNGISQWAEGEPRYALTTTLSARVARLNPTWNHPD
  QDTEAGFKRAMDLVQEEFLQRLDFYQHSWLPARALVEEALAQRFQVDPSGEIVELAKGACPWKEHLYHLE
  SGLSPPVAIFFVIYTDQAGQWRIQCVPKEPHSFQSRLPLPEPWRGLRDEALDQVSGIPGCIFVHASGFIG
  GHRTREGALSMARATLAQRSYLPQIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021640
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021640.2, NP_067653.2
• RefSeq Size: 1215 bp
• RefSeq ORF: 1131 bp
• Locus ID: 60314
• UniProt ID: Q86UA3
• Cytogenetics: 12q13.13
• MW: 42.5 kDa
• Gene Summary: 3'-5' RNA exonuclease which cleaves in situ on specific transcripts in both nucleus and mitochondrion. Involved in regulating spatially segregated organellar RNA processing, acts as a coordinator of nucleo-mitochondrial crosstalk (PubMed:31081026). In nucleolus, processes pre-ribosomal RNA involved in ribosome assembly and alters cytoplasmic translation. In mitochondrial matrix, processes 3'-termini of the mito-ribosomal and messenger RNAs and controls translation of mitochondrial proteins (Probable).[UniProtKB/Swiss-Prot Function]