ROR beta (RORB) (NM_006914) Human Tagged ORF Clone

CAT#: RC208666

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RORB (Myc-DDK-tagged)-Human RAR-related orphan receptor B (RORB)



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RORB
Synonyms bA133M9.1; EIG15; NR1F2; ROR-BETA; RZR-BETA; RZRB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208666 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGAGCACAAATTGAAGTGATACCATGCAAAATTTGTGGCGATAAGTCCTCTGGGATCCACTACGGAG
TCATCACATGTGAAGGCTGCAAGGGATTCTTTAGGAGGAGCCAGCAGAACAATGCTTCTTATTCCTGCCC
AAGGCAGAGAAACTGTTTAATTGACAGAACGAACAGAAACCGTTGCCAACACTGCCGACTGCAGAAGTGT
CTTGCCCTAGGAATGTCAAGAGATGCTGTGAAGTTTGGGAGAATGTCCAAGAAGCAAAGGGACAGCCTGT
ATGCTGAGGTGCAGAAGCACCAGCAGCGGCTGCAGGAACAGCGGCAGCAGCAGAGTGGGGAGGCAGAAGC
CCTTGCCAGGGTGTACAGCAGCAGCATTAGCAACGGCCTGAGCAACCTGAACAACGAGACCAGCGGCACT
TATGCCAACGGGCACGTCATTGACCTGCCCAAGTCTGAGGGTTATTACAACGTCGATTCCGGTCAGCCGT
CCCCTGATCAGTCAGGACTTGACATGACTGGAATCAAACAGATAAAGCAAGAACCTATCTATGACCTCAC
ATCCGTACCCAACTTGTTTACCTATAGCTCTTTCAACAATGGGCAGTTAGCACCAGGGATAACCATGACT
GAAATCGACCGAATTGCACAGAACATCATTAAGTCCCATTTGGAGACATGTCAATACACCATGGAAGAGC
TGCACCAGCTGGCGTGGCAGACCCACACCTATGAAGAAATTAAAGCATATCAAAGCAAGTCCAGGGAAGC
ACTGTGGCAACAATGTGCCATCCAGATCACTCACGCCATCCAATACGTGGTGGAGTTTGCAAAGCGGATA
ACAGGCTTCATGGAGCTCTGTCAAAATGATCAAATTCTACTTCTGAAGTCAGGTTGCTTGGAAGTGGTTT
TAGTGAGAATGTGCCGTGCCTTCAACCCATTAAACAACACTGTTCTGTTTGAAGGAAAATATGGAGGAAT
GCAAATGTTCAAAGCCTTAGGTTCTGATGACCTAGTGAATGAAGCATTTGACTTTGCAAAGAATTTGTGT
TCCTTGCAGCTGACCGAGGAGGAGATCGCTTTGTTCTCATCTGCTGTTCTGATATCTCCAGACCGAGCCT
GGCTTATAGAACCAAGGAAAGTCCAGAAGCTTCAGGAAAAAATTTATTTTGCACTTCAACATGTGATTCA
GAAGAATCACCTGGATGATGAGACCTTGGCAAAGTTAATAGCCAAGATACCAACCATCACGGCAGTTTGC
AACTTGCACGGGGAGAAGCTGCAGGTATTTAAGCAATCTCATCCAGAGATAGTGAATACACTGTTTCCTC
CGTTATACAAGGAGCTCTTTAATCCTGACTGTGCCACCGGCTGCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208666 protein sequence
Red=Cloning site Green=Tags(s)

MRAQIEVIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQNNASYSCPRQRNCLIDRTNRNRCQHCRLQKC
LALGMSRDAVKFGRMSKKQRDSLYAEVQKHQQRLQEQRQQQSGEAEALARVYSSSISNGLSNLNNETSGT
YANGHVIDLPKSEGYYNVDSGQPSPDQSGLDMTGIKQIKQEPIYDLTSVPNLFTYSSFNNGQLAPGITMT
EIDRIAQNIIKSHLETCQYTMEELHQLAWQTHTYEEIKAYQSKSREALWQQCAIQITHAIQYVVEFAKRI
TGFMELCQNDQILLLKSGCLEVVLVRMCRAFNPLNNTVLFEGKYGGMQMFKALGSDDLVNEAFDFAKNLC
SLQLTEEEIALFSSAVLISPDRAWLIEPRKVQKLQEKIYFALQHVIQKNHLDDETLAKLIAKIPTITAVC
NLHGEKLQVFKQSHPEIVNTLFPPLYKELFNPDCATGCK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006914
ORF Size 1377 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006914.4
RefSeq Size 3604 bp
RefSeq ORF 1380 bp
Locus ID 6096
Cytogenetics 9q21.13
Domains HOLI, zf-C4
Protein Families Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
MW 52.1 kDa
Gene Summary The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It is a DNA-binding protein that can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, and to help regulate the expression of some genes involved in circadian rhythm. [provided by RefSeq, Feb 2014]
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