LEF1 (NM_016269) Human Tagged ORF Clone
LEF1 (Myc-DDK-tagged)-Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1
|Product Name||LEF1 (NM_016269) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||LEF-1; TCF1ALPHA; TCF7L3; TCF10|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC208663 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC208663 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1197 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_016269.1, NM_016269.2, NM_016269.3, NM_016269.4, NP_057353.1|
|RefSeq Size||3620 bp|
|RefSeq ORF||1200 bp|
|Protein Families||Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors|
|Protein Pathways||Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway|
|Gene Summary||This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]|
|The use of this cDNA Clones has been cited in the following citations:|
Transplantation of hMSCs Genome Edited with LEF1 Improves Cardio-Protective Effects in Myocardial Infarction
,Cho, H;Lee, K;Shen, Y;Shin, T;Ryu, P;Choi, M;Kang, K;Cho, J;,
Molecular Therapy - Nucleic Acids 2020
,PubMed ID 32069701
Epstein-Barr virus stably confers an invasive phenotype to epithelial cells through reprogramming of the WNT pathway
,Birdwell, CE;Prasai, K;Dykes, S;Jia, Y;Munroe, TGC;Bienkowska-Haba, M;Scott, RS;,
,PubMed ID 29535816
Non-Canonical Wnt Predominates in Activated Rat Hepatic Stellate Cells, Influencing HSC Survival and Paracrine Stimulation of Kupffer Cells
,Corbett, L;Mann, J;Mann, DA;,
PLoS ONE Nov 2015
,PubMed ID 26566235
Regulation of the Bone-restricted IFITM-like (Bril) Gene Transcription by Sp and Gli Family Members and CpG Methylation
,Bahar Kasaai, Marie-Hélène Gaumond, and Pierre Moffatt,
J. Biol. Chem., May 2013; 288: 13278 - 13294.
,PubMed ID 23530031
|cDNA Clone Resources|
|SC114370||LEF1 (untagged)-Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1||
|RG208663||LEF1 (GFP-tagged) - Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1||
|RC208663L1||Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, Myc-DDK-tagged||
|RC208663L2||Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, mGFP tagged||
|RC208663L3||Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, Myc-DDK-tagged||
|RC208663L4||Lenti ORF clone of Human lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, mGFP tagged||