STAT2 (NM_005419) Human Tagged ORF Clone

CAT#: RC208592

STAT2 (Myc-DDK-tagged)-Human signal transducer and activator of transcription 2, 113kDa (STAT2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417313) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208592 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STAT2 protein (Cat# TP308592). The protein was produced from HEK293T cells transfected with STAT2 cDNA clone (Cat# RC208592) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STAT2
• Synonyms: IMD44; ISGF-3; P113; PTORCH3; STAT113
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208592 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGTGGGAAATGCTGCAGAATCTTGACAGCCCCTTTCAGGATCAGCTGCACCAGCTTTACTCGC
  ACAGCCTCCTGCCTGTGGACATTCGACAGTACTTGGCTGTCTGGATTGAAGACCAGAACTGGCAGGAAGC
  TGCACTTGGGAGTGATGATTCCAAGGCTACCATGCTATTCTTCCACTTCTTGGATCAGCTGAACTATGAG
  TGTGGCCGTTGCAGCCAGGACCCAGAGTCCTTGTTGCTGCAGCACAATTTGCGGAAATTCTGCCGGGACA
  TTCAGCCCTTTTCCCAGGATCCTACCCAGTTGGCTGAGATGATCTTTAACCTCCTTCTGGAAGAAAAAAG
  AATTTTGATCCAGGCTCAGAGGGCCCAATTGGAACAAGGAGAGCCAGTTCTCGAAACACCTGTGGAGAGC
  CAGCAACATGAGATTGAATCCCGGATCCTGGATTTAAGGGCTATGATGGAGAAGCTGGTAAAATCCATCA
  GCCAACTGAAAGACCAGCAGGATGTCTTCTGCTTCCGATATAAGATCCAGGCCAAAGGGAAGACACCCTC
  TCTGGACCCCCATCAGACCAAAGAGCAGAAGATTCTGCAGGAAACTCTCAATGAACTGGACAAAAGGAGA
  AAGGAGGTGCTGGATGCCTCCAAAGCACTGCTAGGCCGATTAACTACCCTAATCGAGCTACTGCTGCCAA
  AGTTGGAGGAGTGGAAGGCCCAGCAGCAAAAAGCCTGCATCAGAGCTCCCATTGACCACGGGTTGGAACA
  GCTGGAGACATGGTTCACAGCTGGAGCAAAGCTGTTGTTTCACCTGAGGCAGCTGCTGAAGGAGCTGAAG
  GGACTGAGTTGCCTGGTTAGCTATCAGGATGACCCTCTGACCAAAGGGGTGGACCTACGCAACGCCCAGG
  TCACAGAGTTGCTACAGCGTCTGCTCCACAGAGCCTTTGTGGTAGAAACCCAGCCCTGCATGCCCCAAAC
  TCCCCATCGACCCCTCATCCTCAAGACTGGCAGCAAGTTCACCGTCCGAACAAGGCTGCTGGTGAGACTC
  CAGGAAGGCAATGAGTCACTGACTGTGGAAGTCTCCATTGACAGGAATCCTCCTCAATTACAAGGCTTCC
  GGAAGTTCAACATTCTGACTTCAAACCAGAAAACTTTGACCCCCGAGAAGGGGCAGAGTCAGGGTTTGAT
  TTGGGACTTTGGTTACCTGACTCTGGTGGAGCAACGTTCAGGTGGTTCAGGAAAGGGCAGCAATAAGGGG
  CCACTAGGTGTGACAGAGGAACTGCACATCATCAGCTTCACGGTCAAATATACCTACCAGGGTCTGAAGC
  AGGAGCTGAAAACGGACACCCTCCCTGTGGTGATTATTTCCAACATGAACCAGCTCTCAATTGCCTGGGC
  TTCAGTTCTCTGGTTCAATTTGCTCAGCCCAAACCTTCAGAACCAGCAGTTCTTCTCCAACCCCCCCAAG
  GCCCCCTGGAGCTTGCTGGGCCCTGCTCTCAGTTGGCAGTTCTCCTCCTATGTTGGCCGAGGCCTCAACT
  CAGACCAGCTGAGCATGCTGAGAAACAAGCTGTTCGGGCAGAACTGTAGGACTGAGGATCCATTATTGTC
  CTGGGCTGACTTCACTAAGCGAGAGAGCCCTCCTGGCAAGTTACCATTCTGGACATGGCTGGACAAAATT
  CTGGAGTTGGTACATGACCACCTGAAGGATCTCTGGAATGATGGACGCATCATGGGCTTTGTGAGTCGGA
  GCCAGGAGCGCCGGCTGCTGAAGAAGACCATGTCTGGCACCTTTCTACTGCGCTTCAGTGAATCGTCAGA
  AGGGGGCATTACCTGCTCCTGGGTGGAGCACCAGGATGATGACAAGGTGCTCATCTACTCTGTGCAACCG
  TACACGAAGGAGGTGCTGCAGTCACTCCCGCTGACTGAAATCATCCGCCATTACCAGTTGCTCACTGAGG
  AGAATATACCTGAAAACCCACTGCGCTTCCTCTATCCCCGAATCCCCCGGGATGAAGCTTTTGGGTGCTA
  CTACCAGGAGAAAGTTAATCTCCAGGAACGGAGGAAATACCTGAAACACAGGCTCATTGTGGTCTCTAAT
  AGACAGGTGGATGAACTGCAACAACCGCTGGAGCTTAAGCCAGAGCCAGAGCTGGAGTCATTAGAGCTGG
  AACTAGGGCTGGTGCCAGAGCCAGAGCTCAGCCTGGACTTAGAGCCACTGCTGAAGGCAGGGCTGGATCT
  GGGGCCAGAGCTAGAGTCTGTGCTGGAGTCCACTCTGGAGCCTGTGATAGAGCCCACACTATGCATGGTA
  TCACAAACAGTGCCAGAGCCAGACCAAGGACCTGTATCACAGCCAGTGCCAGAGCCAGATTTGCCCTGTG
  ATCTGAGACATTTGAACACTGAGCCAATGGAAATCTTCAGAAACTGTGTAAAGATTGAAGAAATCATGCC
  GAATGGTGACCCACTGTTGGCTGGCCAGAACACCGTGGATGAGGTTTACGTCTCCCGCCCCAGCCACTTC
  TACACTGATGGACCCTTGATGCCTTCTGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208592 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQWEMLQNLDSPFQDQLHQLYSHSLLPVDIRQYLAVWIEDQNWQEAALGSDDSKATMLFFHFLDQLNYE
  CGRCSQDPESLLLQHNLRKFCRDIQPFSQDPTQLAEMIFNLLLEEKRILIQAQRAQLEQGEPVLETPVES
  QQHEIESRILDLRAMMEKLVKSISQLKDQQDVFCFRYKIQAKGKTPSLDPHQTKEQKILQETLNELDKRR
  KEVLDASKALLGRLTTLIELLLPKLEEWKAQQQKACIRAPIDHGLEQLETWFTAGAKLLFHLRQLLKELK
  GLSCLVSYQDDPLTKGVDLRNAQVTELLQRLLHRAFVVETQPCMPQTPHRPLILKTGSKFTVRTRLLVRL
  QEGNESLTVEVSIDRNPPQLQGFRKFNILTSNQKTLTPEKGQSQGLIWDFGYLTLVEQRSGGSGKGSNKG
  PLGVTEELHIISFTVKYTYQGLKQELKTDTLPVVIISNMNQLSIAWASVLWFNLLSPNLQNQQFFSNPPK
  APWSLLGPALSWQFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKI
  LELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQP
  YTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSN
  RQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMV
  SQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHF
  YTDGPLMPSDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005419
• ORF Size: 2553 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005419.4
• RefSeq Size: 4576 bp
• RefSeq ORF: 2556 bp
• Locus ID: 6773
• UniProt ID: P52630
• Cytogenetics: 12q13.3
• Domains: SH2, STAT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Jak-STAT signaling pathway
• MW: 97.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. The protein mediates innate antiviral activity. Mutations in this gene result in Immunodeficiency 44. [provided by RefSeq, Aug 2020]