MTHFR (NM_005957) Human Tagged ORF Clone
Lenti ORF clone of Human methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), Myc-DDK-tagged
|Type||Human Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Mammalian Cell Selection||Puromycin|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1968 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||6099 bp|
|RefSeq ORF||1971 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||Metabolic pathways, Methane metabolism, One carbon pool by folate|
|Gene Summary||The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]|
|cDNA Clone Resources|
|RC208588||MTHFR (Myc-DDK-tagged)-Human methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR)||
|RC208588L1||Lenti ORF clone of Human methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), Myc-DDK-tagged||
|RC208588L2||Lenti ORF clone of Human methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), mGFP tagged||
|RC208588L4||Lenti ORF clone of Human methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), mGFP tagged||
|RG208588||MTHFR (GFP-tagged) - Human methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR)||
|SC125935||MTHFR (untagged)-Human methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR)||