NT5DC3 (NM_001031701) Human Tagged ORF Clone

CAT#: RC208580

NT5DC3 (Myc-DDK-tagged)-Human 5'-nucleotidase domain containing 3 (NT5DC3)

Product Images

Circular map for RC208580

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NT5DC3
• Synonyms: TU12B1-TY; TU12B1TY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208580 representing NM_001031701
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGGCAGCGGCGGCGGTGGTGGCACGCGGGGCCGGGGCGAGGGCAGCGACAGCGGCGGCTTTGC
  GGGGTGGCTGCGGGACCGCGGCTCGGGGGCGGCCGTGTGCGGGCCCCGCCCGGCCCTTGTGCACTGCACC
  CGGGACCGCCCCGGACATGAAGCGCTACCTGTGGGAGCGCTACCGGGAGGCGAAGAGAAGCACAGAAGAA
  TTGGTTCCTTCCATTATGAGCAACTTGTTGAATCCAGATGCCATTTTCTCAAACAATGAAATGAGCCTGT
  CAGACATTGAAATCTATGGCTTCGATTATGATTACACCTTGGTGTTTTATTCAAAGCACCTCCACACGCT
  GATATTTAATGCTGCACGGGACCTTCTCATCAATGAACACCGGTATCCAGCAGAAATCAGGAAGTATGAG
  TATGACCCAAATTTTGCAATTCGTGGACTTCATTATGATGTACAGCGGGCAGTATTAATGAAGATCGATG
  CTTTTCATTATATCCAGCTGGGAACTGTCTACAGAGGCCTCAGTGTTGTCCCTGATGAAGAAGTCATTGA
  AATGTACGAGGGGTCCCACGTGCCCTTGGAGCAGATGAGTGACTTTTACGGAAAGAGCTCTCATGGAAAC
  ACGATGAAGCAGTTCATGGACATCTTCTCCCTGCCCGAGATGACCCTCCTGTCCTGCGTGAATGAATACT
  TCCTCAAGAACAACATCGACTATGAGCCTGTGCATCTGTACAAAGATGTCAAGGATTCAATTCGAGACGT
  CCACATCAAAGGAATAATGTACAGAGCAATTGAAGCAGACATTGAAAAGTACATCTGCTATGCTGAGCAG
  ACCCGCGCAGTGTTGGCCAAACTGGCTGATCATGGCAAGAAGATGTTTCTCATCACCAATAGCCCCAGTA
  GCTTTGTGGACAAAGGGATGAGTTATATCGTTGGGAAAGACTGGAGGGACCTGTTCGATGTGGTCATTGT
  TCAGGCTGAGAAGCCAAACTTCTTTAATGATAAGCGGAGGCCTTTCCGAAAGATGAATGAGAAAGGTGTC
  TTACTCTGGGATAAAATCCATAAGTTGCAGAAAGGCCAGATATACAAGCAGGGTAATTTATATGAATTTT
  TGAAGCTTACTGGATGGAGAGGATCCAGAGTGTTGTATTTTGGTGACCATATATACAGTGACCTGGCGGA
  TTTGACCCTAAAGCATGGCTGGAGGACTGGTGCAATCATCCCAGAGTTGAGATCTGAGCTCAAAATCATG
  AACACGGAGCAATACATTCAAACCATGACCTGGCTGCAGACCTTGACTGGCTTATTGGAACAGATGCAGG
  TTCACAGAGATGCTGAGTCACAGCTGGTTTTGCAGGAGTGGAAAAAGGAAAGGAAGGAGATGCGAGAAAT
  GACCAAGAGTTTCTTCAATGCCCAGTTTGGAAGCCTGTTCCGCACAGACCAGAACCCAACCTACTTCCTA
  AGGCGCCTGTCGCGCTTCGCTGACATCTACATGGCGTCTCTGAGCTGCCTCCTGAACTATGACGTCAGCC
  ACACTTTCTACCCCCGGAGGACTCCACTGCAGCACGAACTGCCCGCCTGGTCAGAAAGGCCCCCCACCTT
  CGGAACCCCTCTCCTGCAGGAGGCCCAGGCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208580 representing NM_001031701
  Red=Cloning site Green=Tags(s)
  
  MTMAAAAVVARGAGARAATAAALRGGCGTAARGRPCAGPARPLCTAPGTAPDMKRYLWERYREAKRSTEE
  LVPSIMSNLLNPDAIFSNNEMSLSDIEIYGFDYDYTLVFYSKHLHTLIFNAARDLLINEHRYPAEIRKYE
  YDPNFAIRGLHYDVQRAVLMKIDAFHYIQLGTVYRGLSVVPDEEVIEMYEGSHVPLEQMSDFYGKSSHGN
  TMKQFMDIFSLPEMTLLSCVNEYFLKNNIDYEPVHLYKDVKDSIRDVHIKGIMYRAIEADIEKYICYAEQ
  TRAVLAKLADHGKKMFLITNSPSSFVDKGMSYIVGKDWRDLFDVVIVQAEKPNFFNDKRRPFRKMNEKGV
  LLWDKIHKLQKGQIYKQGNLYEFLKLTGWRGSRVLYFGDHIYSDLADLTLKHGWRTGAIIPELRSELKIM
  NTEQYIQTMTWLQTLTGLLEQMQVHRDAESQLVLQEWKKERKEMREMTKSFFNAQFGSLFRTDQNPTYFL
  RRLSRFADIYMASLSCLLNYDVSHTFYPRRTPLQHELPAWSERPPTFGTPLLQEAQAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031701
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031701.3
• RefSeq Size: 7214 bp
• RefSeq ORF: 1647 bp
• Locus ID: 51559
• UniProt ID: Q86UY8
• Cytogenetics: 12q23.3
• MW: 63.4 kDa