LSM12 (NM_152344) Human Tagged ORF Clone

SKU
RC208457
LSM12 (Myc-DDK-tagged)-Human LSM12 homolog (S. cerevisiae) (LSM12)
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$300.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LSM12
Synonyms PNAS-135
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208457 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTCCTCCGGGCGAGTACTTCAGCGTTGGGAGCCAGGTGTCGTGCCGGACGTGCCAGGAGCAGC
GGCTGCAGGGCGAGGTGGTAGCCTTTGACTACCAATCCAAAATGCTGGCTTTAAAATGTCCCTCTTCCAG
TGGAAAGCCCAACCATGCAGACATCTTGCTCATAAACTTACAGTATGTTTCAGAAGTGGAAATAATTAAT
GACCGAACAGAAACCCCTCGTCCCCTAGCTTCACTCAATGTTAGTAAGCTTGCCAGCAAAGCACGGACAG
AGAAGGAGGAGAAGCTGAGCCAGGCCTATGCAATCAGTGCTGGTGTCTCTCTAGAGGGCCAGCAGCTCTT
CCAGACCATTCACAAGACCATTAAAGACTGTAAATGGCAAGAAAAAAACATCGTAGTCATGGAAGAAGTT
GTTATTACACCCCCATATCAAGTGGAAAACTGTAAAGGCAAAGAGGGGAGTGCACTGAGCCATGTACGCA
AAATAGTTGAAAAACATTTTAGAGACGTGGAAAGCCAAAAGATACTGCAACGTTCACAAGCCCAGCAACC
ACAGAAGGAGGCTGCCCTGTCATCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC208457 protein sequence
Red=Cloning site Green=Tags(s)

MAAPPGEYFSVGSQVSCRTCQEQRLQGEVVAFDYQSKMLALKCPSSSGKPNHADILLINLQYVSEVEIIN
DRTETPRPLASLNVSKLASKARTEKEEKLSQAYAISAGVSLEGQQLFQTIHKTIKDCKWQEKNIVVMEEV
VITPPYQVENCKGKEGSALSHVRKIVEKHFRDVESQKILQRSQAQQPQKEAALSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_152344
ORF Size 585 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152344.4
RefSeq Size 2507 bp
RefSeq ORF 588 bp
Locus ID 124801
UniProt ID Q3MHD2
Cytogenetics 17q21.31
MW 21.8 kDa
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SKU Description Size Price
RC208457L3 Lenti ORF clone of Human LSM12 homolog (S. cerevisiae) (LSM12), Myc-DDK-tagged 10 ug
$600.00
RC208457L4 Lenti ORF clone of Human LSM12 homolog (S. cerevisiae) (LSM12), mGFP tagged 10 ug
$600.00
RG208457 LSM12 (tGFP-tagged) - Human LSM12 homolog (S. cerevisiae) (LSM12) 10 ug
$489.00 MSRP $500.00 MSRP $500.00
SC100571 LSM12 (untagged)-Human LSM12 homolog (S. cerevisiae) (LSM12) 10 ug
$300.00

Citations

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