DVL1 (NM_182779) Human Tagged ORF Clone

CAT#: RC208406

DVL1 (Myc-DDK-tagged)-Human dishevelled, dsh homolog 1 (Drosophila) (DVL1), transcript variant 3

Product Images

Circular map for RC208406

• 

  Western blot validation of overexpression lysate (Cat# LY405356) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208406 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DVL1
• Synonyms: dishevelled, dsh homolog 1 (Drosophila); dishevelled 1; dishevelled 1 (homologous to Drosophila dsh); DVL; DVL, MGC54245; MGC54245; OTTHUMP00000003104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208406 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACCAAGATTATCTACCACATGGACGAGGAGGAGACGCCGTACCTGGTCAAGCTGCCCGTGG
  CCCCCGAGCGCGTCACGCTGGCCGACTTCAAGAACGTGCTCAGCAACCGGCCCGTGCACGCCTACAAATT
  CTTCTTTAAGTCCATGGACCAGGACTTCGGGGTGGTGAAGGAGGAGATCTTTGATGACAATGCCAAGCTT
  CCCTGCTTCAACGGCCGCGTGGTCTCCTGGCTGGTCCTGGCTGAGGGTGCTCACTCGGATGCGGGGTCCC
  AGGGCACGGACAGCCACACAGACCTGCCCCCGCCTCTTGAGCGGACAGGCGGCATCGGGGACTCCCGGCC
  CCCCTCCTTCCACCCGAATGTGGCCAGCAGCCGTGACGGGATGGACAACGAGACAGGCACGGAGTCCATG
  GTCAGTCACCGGCGGGAGCGTGCCCGACGCCGGAACCGCGAGGAGGCCGCCCGGACCAATGGGCACCCAA
  GGGGAGACCGACGGCGGGATGTGGGGCTGCCCCCAGACAGCGCGTCCACCGCCCTCAGCAGCGAGCTTGA
  GTCCAGCAGCTTTGTGGACTCGGACGAGGATGGCAGCACGAGCAGGCTCAGCAGCTCCACGGAGCAGAGC
  ACCTCATCCAGACTCACCCGGAAGTACGCCAGCAGCTTGCTGAAGCACGGCTTCCTGCGGCACACGGTCA
  ACAAGATCACCTTCTCCGAGCAGTGCTACTACGTCTTCGGGGATCTCTGCAGCAATCTCGCCACCCTGAA
  CCTCAACAGTGGCTCCAGTGGGACTTCGGATCAGGACACGCTGGCCCCGCTGCCCCACCCGGCTGCCCCC
  TGGCCTCTGGGTCAGGGCTACCCCTACCAGTACCCGGGACCCCCACCCTGCTTCCCGCCTGCCTACCAGG
  ACCCGGGCTTTAGCTATGGCAGCGGCAGCACCGGGAGTCAGCAGAGTGAAGGGAGCAAAAGCAGTGGGTC
  CACCCGGAGCAGCCGCCGGGCCCCGGGCCGTGAGAAGGAGCGTCGGGCGGCGGGAGCTGGGGGCAGTGGC
  AGTGAATCGGATCACACGGCACCGAGTGGGGTGGGGAGCAGCTGGCGAGAGCGTCCGGCCGGCCAGCTCA
  GCCGTGGCAGCAGCCCACGCAGTCAGGCCTCGGCTACCGCCCCGGGGCTCCCCCCGCCCCACCCCACGAC
  CAAGGCCTATACAGTGGTGGGGGGGCCACCCGGGGGACCCCCTGTCCGGGAGCTGGCTGCCGTCCCCCCG
  GAATTGACAGGCAGCCGCCAGTCCTTCCAGAAGGCTATGGGGAACCCCTGCGAGTTCTTCGTGGACATCA
  TG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208406 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKL
  PCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESM
  VSHRRERARRRNREEAARTNGHPRGDRRRDVGLPPDSASTALSSELESSSFVDSDEDGSTSRLSSSTEQS
  TSSRLTRKYASSLLKHGFLRHTVNKITFSEQCYYVFGDLCSNLATLNLNSGSSGTSDQDTLAPLPHPAAP
  WPLGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSG
  SESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRELAAVPP
  ELTGSRQSFQKAMGNPCEFFVDIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182779
• ORF Size: 1332 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182779.2, NP_877580.1
• RefSeq Size: 2266 bp
• RefSeq ORF: 1334 bp
• Locus ID: 1855
• Cytogenetics: 1p36.33
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• MW: 47.7 kDa
• Gene Summary: DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development. [provided by RefSeq, Jul 2008]