Fetuin A (AHSG) (NM_001622) Human Tagged ORF Clone

CAT#: RC208344

AHSG (Myc-DDK-tagged)-Human alpha-2-HS-glycoprotein (AHSG)

Product Images

Circular map for RC208344

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AHSG (Cat# RC208344, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AHSG(Cat# TA504670). Positive lysates LY400610 (100ug) and LC400610 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY400610) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208344 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified AHSG protein (Cat# TP308344). The protein was produced from HEK293T cells transfected with AHSG cDNA clone (Cat# RC208344) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fetuin A
• Synonyms: A2HS; AHS; APMR1; FETUA; HSGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208344 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCCCTCGTCCTGCTCCTTTGTCTTGCTCAGCTGTGGGGCTGCCACTCAGCCCCACATGGCCCAG
  GGCTGATTTATAGACAACCGAACTGCGATGATCCAGAAACTGAGGAAGCAGCTCTGGTGGCTATAGACTA
  CATCAATCAAAACCTTCCTTGGGGATACAAACACACCTTGAACCAGATTGATGAAGTAAAGGTGTGGCCT
  CAGCAGCCCTCCGGAGAGCTGTTTGAGATTGAAATAGACACCCTGGAAACCACCTGCCATGTGCTGGACC
  CCACCCCTGTGGCAAGATGCAGCGTGAGGCAGCTGAAGGAGCATGCTGTCGAAGGAGACTGTGATTTCCA
  GCTGTTGAAACTAGATGGCAAGTTTTCCGTGGTATACGCAAAATGTGATTCCAGTCCAGACTCAGCCGAG
  GACGTGCGCAAGGTGTGCCAAGACTGCCCCCTGCTGGCCCCGCTGAACGACACCAGGGTGGTGCACGCCG
  CGAAAGCTGCCCTGGCCGCCTTCAACGCTCAGAACAACGGCTCCAATTTTCAGCTGGAGGAAATTTCCCG
  GGCTCAGCTTGTGCCCCTCCCACCTTCTACCTATGTGGAGTTTACAGTGTCTGGCACTGACTGTGTTGCT
  AAAGAGGCCACAGAGGCAGCCAAGTGTAACCTGCTGGCAGAAAAGCAATATGGCTTTTGTAAGGCAACAC
  TCAGTGAGAAGCTTGGTGGGGCAGAGGTTGCAGTGACCTGCACGGTGTTCCAAACACAGCCCGTGACCTC
  ACAGCCCCAACCAGAAGGTGCCAATGAAGCAGTCCCCACCCCCGTGGTGGACCCAGATGCACCTCCGTCC
  CCTCCACTTGGCGCACCTGGACTCCCTCCAGCTGGCTCACCCCCAGACTCCCATGTGTTACTGGCAGCTC
  CTCCAGGACACCAGTTGCACCGGGCGCACTACGACCTGCGCCACACCTTCATGGGTGTGGTCTCATTGGG
  GTCACCCTCAGGAGAAGTGTCGCACCCCCGGAAAACACGCACAGTGGTGCAGCCTAGTGTTGGTGCTGCT
  GCTGGGCCAGTGGTTCCTCCATGTCCGGGGAGGATCAGACACTTCAAGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208344 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKSLVLLLCLAQLWGCHSAPHGPGLIYRQPNCDDPETEEAALVAIDYINQNLPWGYKHTLNQIDEVKVWP
  QQPSGELFEIEIDTLETTCHVLDPTPVARCSVRQLKEHAVEGDCDFQLLKLDGKFSVVYAKCDSSPDSAE
  DVRKVCQDCPLLAPLNDTRVVHAAKAALAAFNAQNNGSNFQLEEISRAQLVPLPPSTYVEFTVSGTDCVA
  KEATEAAKCNLLAEKQYGFCKATLSEKLGGAEVAVTCTVFQTQPVTSQPQPEGANEAVPTPVVDPDAPPS
  PPLGAPGLPPAGSPPDSHVLLAAPPGHQLHRAHYDLRHTFMGVVSLGSPSGEVSHPRKTRTVVQPSVGAA
  AGPVVPPCPGRIRHFKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001622
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001622.4
• RefSeq Size: 1594 bp
• RefSeq ORF: 1104 bp
• Locus ID: 197
• UniProt ID: P02765
• Cytogenetics: 3q27.3
• Domains: CY
• Protein Families: Druggable Genome, Secreted Protein
• MW: 39.3 kDa
• Gene Summary: The protein encoded by this gene is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including endocytosis, brain development, and the formation of bone tissue. Defects in this gene are a cause of susceptibility to leanness. [provided by RefSeq, Aug 2017]