Deltex (DTX1) (NM_004416) Human Tagged ORF Clone

CAT#: RC208338

DTX1 (Myc-DDK-tagged)-Human deltex homolog 1 (Drosophila) (DTX1)

Product Images

Circular map for RC208338

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Deltex
• Synonyms: hDx-1; RNF140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208338 representing NM_004416
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACGGCCAGGCCACGGTGGGCTGATGCCTGTGAATGGTCTGGGCTTCCCACCGCAGAACGTGGCCC
  GGGTGGTGGTGTGGGAGTGGCTGAATGAGCACAGCCGCTGGCGGCCCTACACGGCCACCGTGTGCCACCA
  CATTGAGAACGTGCTGAAGGAGGACGCTCGCGGTTCCGTGGTCCTGGGGCAGGTGGACGCCCAGCTTGTG
  CCCTACATCATCGACCTGCAGTCCATGCACCAGTTTCGCCAGGACACAGGCACCATGCGGCCCGTGCGGC
  GCAACTTCTACGACCCGTCGTCGGCGCCGGGCAAGGGCATCGTGTGGGAGTGGGAGAACGACGGCGGCGC
  ATGGACGGCCTACGATATGGACATCTGCATCACCATCCAGAACGCCTACGAGAAGCAGCACCCGTGGCTC
  GACCTCTCATCGCTAGGCTTCTGCTACCTCATCTACTTCAACAGCATGTCGCAGATGAACCGCCAGACGC
  GCCGGCGCCGCCGCCTGCGCCGCCGCCTGGACCTCGCCTACCCGCTCACCGTGGGCTCCATCCCTAAGTC
  GCAGTCGTGGCCCGTGGGCGCCAGCTCGGGCCAGCCCTGCTCCTGCCAGCAGTGCCTGCTGGTCAACAGC
  ACGCGCGCCGCCTCCAACGCCATCCTGGCCTCGCAGCGCCGCAAGGCGCCCCCCGCGCCCCCGCTGCCGC
  CGCCGCCGCCACCTGGAGGGCCTCCAGGCGCGCTTGCCGTGCGCCCCAGCGCCACCTTCACAGGCGCCGC
  GCTCTGGGCAGCGCCCGCCGCCGGCCCCGCCGAGCCCGCGCCGCCTCCCGGGGCGCCCCCACGGAGCCCG
  GGCGCCCCCGGCGGAGCGCGCACCCCGGGGCAGAACAACCTCAACCGGCCCGGGCCCCAGCGCACCACCA
  GCGTGAGCGCGCGCGCCTCCATCCCGCCGGGGGTCCCCGCACTCCCGGTGAAGAACTTGAATGGTACTGG
  GCCGGTCCATCCGGCCCTGGCAGGGATGACCGGGATACTGCTGTGCGCGGCCGGGCTGCCCGTGTGCCTG
  ACGCGGGCCCCCAAGCCCATCCTGCACCCGCCGCCCGTGAGCAAGAGCGACGTGAAGCCCGTGCCTGGCG
  TGCCCGGGGTGTGCCGCAAGACCAAGAAGAAGCACCTTAAAAAGAGTAAGAATCCCGAGGATGTGGTTCG
  AAGATACATGCAGAAGGTGAAAAACCCACCTGATGAGGACTGCACCATCTGCATGGAGCGACTGGTCACA
  GCATCAGGCTACGAGGGCGTGCTTCGGCACAAGGGCGTGCGGCCTGAGCTCGTGGGCCGCCTGGGCCGCT
  GTGGCCACATGTACCACCTGCTGTGCCTCGTGGCCATGTACTCCAATGGCAACAAGGATGGCAGCCTGCA
  GTGCCCCACCTGCAAGGCCATCTACGGGGAGAAGACGGGTACGCAGCCGCCTGGGAAGATGGAGTTCCAC
  CTCATCCCCCACTCGCTGCCCGGCTTCCCTGATACCCAGACCATCCGCATCGTCTATGACATCCCCACAG
  GCATCCAGGGCCCTGAGCACCCCAACCCCGGGAAGAAGTTCACCGCAAGAGGATTCCCTCGCCACTGCTA
  TCTACCCAACAACGAGAAAGGCCGGAAGGTGCTGCGGCTGCTCATCACGGCCTGGGAGAGAAGACTCATC
  TTCACTATCGGCACGTCCAACACCACGGGCGAGTCGGACACCGTGGTGTGGAACGAGATCCACCACAAGA
  CCGAGTTTGGATCCAACCTCACGGGCCACGGCTACCCGGACGCTAGCTACCTAGACAACGTGCTGGCTGA
  GCTCACAGCCCAGGGCGTATCCGAGGCTGCAGCCAAGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208338 representing NM_004416
  Red=Cloning site Green=Tags(s)
  
  MSRPGHGGLMPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLV
  PYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWL
  DLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNS
  TRAASNAILASQRRKAPPAPPLPPPPPPGGPPGALAVRPSATFTGAALWAAPAAGPAEPAPPPGAPPRSP
  GAPGGARTPGQNNLNRPGPQRTTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCL
  TRAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVT
  ASGYEGVLRHKGVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTGTQPPGKMEFH
  LIPHSLPGFPDTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLI
  FTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAAAKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004416
• ORF Size: 1860 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004416.2, NP_004407.2
• RefSeq Size: 3317 bp
• RefSeq ORF: 1863 bp
• Locus ID: 1840
• UniProt ID: Q86Y01
• Cytogenetics: 12q24.13
• Domains: RING, WWE
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway
• MW: 67.2 kDa
• Gene Summary: Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. [provided by RefSeq, Jul 2008]