SV2A (NM_014849) Human Tagged ORF Clone

CAT#: RC208322

SV2A (Myc-DDK-tagged)-Human synaptic vesicle glycoprotein 2A (SV2A)

Product Images

Circular map for RC208322

• 

  Western blot validation of overexpression lysate (Cat# LY414979) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208322 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SV2A
• Synonyms: SV2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208322 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGGGCTTCCGAGACCGGGCAGCTTTCATCCGTGGGGCCAAAGACATTGCTAAGGAAGTCAAAA
  AGCATGCGGCCAAGAAGGTGGTGAAGGGCCTGGACAGAGTCCAGGACGAATATTCCCGAAGATCGTACTC
  CCGCTTTGAGGAGGAGGATGATGATGATGACTTCCCTGCTCCCAGTGATGGTTATTACCGAGGAGAAGGG
  ACCCAGGATGAGGAGGAAGGTGGTGCATCCAGTGATGCTACTGAGGGCCATGACGAGGATGATGAGATCT
  ATGAAGGGGAATATCAGGGCATTCCCCGGGCAGAGTCTGGGGGCAAAGGCGAGCGGATGGCAGATGGGGC
  GCCCCTGGCTGGAGTAAGGGGGGGCTTGAGTGATGGGGAGGGTCCCCCTGGGGGCCGGGGGGAGGCACAA
  CGACGGAAAGAACGAGAAGAACTGGCCCAACAGTATGAAGCCATCCTACGGGAGTGTGGCCACGGCCGCT
  TCCAGTGGACACTGTATTTTGTGCTTGGTCTGGCGCTGATGGCTGACGGTGTGGAGGTCTTTGTGGTGGG
  CTTCGTGCTGCCCAGCGCTGAGAAAGACATGTGCCTGTCCGACTCCAACAAAGGCATGCTAGGCCTCATC
  GTCTACCTGGGCATGATGGTGGGAGCCTTCCTCTGGGGAGGTCTGGCTGACCGGCTGGGTCGGAGGCAGT
  GTCTGCTCATCTCGCTCTCAGTCAACAGCGTCTTCGCCTTCTTCTCATCTTTTGTCCAGGGTTACGGCAC
  TTTCCTCTTCTGCCGCCTACTTTCTGGGGTTGGGATTGGAGGGTCCATCCCCATTGTCTTCTCCTATTTC
  TCCGAGTTTCTGGCCCAGGAGAAACGAGGGGAGCATTTGAGCTGGCTCTGCATGTTTTGGATGATTGGTG
  GCGTGTACGCAGCTGCTATGGCCTGGGCCATCATCCCCCACTATGGGTGGAGTTTTCAGATGGGTTCTGC
  CTACCAGTTCCACAGCTGGAGGGTCTTCGTCCTCGTCTGCGCCTTTCCTTCTGTGTTTGCCATTGGGGCT
  CTGACCACGCAGCCTGAGAGCCCCCGTTTCTTCCTAGAGAATGGAAAGCATGATGAGGCCTGGATGGTGC
  TGAAGCAGGTCCATGATACCAACATGCGAGCCAAAGGACATCCTGAGCGAGTGTTCTCAGTAACCCACAT
  TAAGACGATTCATCAGGAGGATGAATTGATTGAGATCCAGTCGGACACAGGGACCTGGTACCAGCGCTGG
  GGGGTCCGGGCCTTGAGCCTAGGGGGGCAGGTTTGGGGGAATTTTCTCTCCTGTTTTGGTCCCGAATATC
  GGCGCATCACTCTGATGATGATGGGTGTGTGGTTCACCATGTCATTCAGCTACTATGGCCTGACCGTCTG
  GTTTCCTGACATGATCCGCCATCTCCAGGCAGTGGACTACGCATCCCGCACCAAAGTGTTCCCCGGGGAG
  CGCGTAGAGCATGTAACTTTTAACTTCACGTTGGAGAATCAGATCCACCGAGGCGGGCAGTACTTCAATG
  ACAAGTTCATTGGGCTGCGGCTCAAGTCAGTGTCCTTTGAGGATTCCCTGTTTGAAGAGTGTTATTTTGA
  GGATGTCACATCCAGCAACACGTTTTTCCGCAACTGCACATTCATCAACACTGTGTTCTATAACACTGAC
  CTGTTCGAGTACAAGTTTGTGAACAGCCGTCTGATAAACAGTACATTCCTGCACAACAAGGAGGGCTGCC
  CGCTAGACGTGACAGGGACGGGCGAAGGTGCCTACATGGTATACTTTGTGAGCTTCCTGGGGACACTGGC
  AGTGCTTCCTGGGAATATCGTGTCTGCCCTGCTCATGGACAAGATCGGCAGGCTCAGAATGCTTGCTGGC
  TCCAGCGTGATGTCCTGTGTCTCCTGCTTCTTCCTGTCTTTTGGGAACAGTGAGTCGGCCATGATCGCTC
  TGCTCTGCCTTTTTGGCGGGGTCAGCATTGCATCCTGGAATGCGCTGGACGTGTTGACTGTTGAACTCTA
  CCCCTCAGACAAGAGGACCACAGCTTTTGGCTTCCTGAATGCCCTGTGTAAGCTGGCAGCTGTGCTGGGG
  ATCAGCATCTTCACATCCTTCGTGGGAATCACCAAGGCTGCACCCATCCTCTTTGCCTCAGCTGCCCTTG
  CCCTTGGCAGCTCTCTGGCCCTGAAGCTGCCTGAGACCCGGGGGCAGGTGCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208322 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGFRDRAAFIRGAKDIAKEVKKHAAKKVVKGLDRVQDEYSRRSYSRFEEEDDDDDFPAPSDGYYRGEG
  TQDEEEGGASSDATEGHDEDDEIYEGEYQGIPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQ
  RRKEREELAQQYEAILRECGHGRFQWTLYFVLGLALMADGVEVFVVGFVLPSAEKDMCLSDSNKGMLGLI
  VYLGMMVGAFLWGGLADRLGRRQCLLISLSVNSVFAFFSSFVQGYGTFLFCRLLSGVGIGGSIPIVFSYF
  SEFLAQEKRGEHLSWLCMFWMIGGVYAAAMAWAIIPHYGWSFQMGSAYQFHSWRVFVLVCAFPSVFAIGA
  LTTQPESPRFFLENGKHDEAWMVLKQVHDTNMRAKGHPERVFSVTHIKTIHQEDELIEIQSDTGTWYQRW
  GVRALSLGGQVWGNFLSCFGPEYRRITLMMMGVWFTMSFSYYGLTVWFPDMIRHLQAVDYASRTKVFPGE
  RVEHVTFNFTLENQIHRGGQYFNDKFIGLRLKSVSFEDSLFEECYFEDVTSSNTFFRNCTFINTVFYNTD
  LFEYKFVNSRLINSTFLHNKEGCPLDVTGTGEGAYMVYFVSFLGTLAVLPGNIVSALLMDKIGRLRMLAG
  SSVMSCVSCFFLSFGNSESAMIALLCLFGGVSIASWNALDVLTVELYPSDKRTTAFGFLNALCKLAAVLG
  ISIFTSFVGITKAAPILFASAALALGSSLALKLPETRGQVLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014849
• ORF Size: 2226 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014849.5
• RefSeq Size: 4428 bp
• RefSeq ORF: 2229 bp
• Locus ID: 9900
• UniProt ID: Q7L0J3
• Cytogenetics: 1q21.2
• Domains: sugar_tr
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: ECM-receptor interaction
• MW: 82.7 kDa
• Gene Summary: The protein encoded by this gene is one of three related synaptic vesicle proteins. The encoded protein may interact with synaptotagmin to enhance low frequency neurotransmission in quiescent neurons. [provided by RefSeq, Jun 2016]