MICAL1 (NM_022765) Human Tagged ORF Clone

CAT#: RC208308

MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1

Product Images

Circular map for RC208308

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MICAL1 (Cat# RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1(Cat# TA501893). Positive lysates LY402940 (100ug) and LC402940 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY402940) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208308 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MICAL1 protein (Cat# TP308308). The protein was produced from HEK293T cells transfected with MICAL1 cDNA clone (Cat# RC208308) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MICAL1
• Synonyms: MICAL; MICAL-1; NICAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208308 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCACCTACCTCCACCAACCCAGCGCATGCCCACTTTGAGAGCTTCCTGCAGGCCCAGCTGTGCC
  AGGACGTGCTGAGCAGCTTCCAGGAGCTGTGTGGGGCCCTGGGGCTGGAACCCGGTGGGGGGCTGCCCCA
  GTACCACAAGATCAAGGACCAGCTCAACTACTGGAGCGCCAAGTCACTGTGGACCAAGCTGGACAAGCGA
  GCAGGCCAGCCTGTCTACCAGCAGGGCCGGGCCTGCACCAGCACCAAGTGCCTGGTGGTGGGTGCTGGAC
  CTTGCGGGCTGCGGGTCGCTGTGGAGCTGGCGCTGCTGGGGGCCCGAGTGGTGCTGGTGGAAAAGCGCAC
  CAAGTTCTCTCGCCACAACGTGCTCCACCTCTGGCCCTTCACCATCCACGACCTGCGGGCACTCGGTGCT
  AAGAAGTTCTACGGGCGCTTCTGCACCGGCACCCTGGACCACATCAGCATCAGGCAGCTCCAGCTGCTTC
  TGCTGAAGGTAGCATTGCTGCTGGGGGTGGAAATTCACTGGGGTGTCACTTTCACTGGCCTCCAGCCCCC
  TCCTAGGAAGGGGAGTGGCTGGCGTGCCCAGCTCCAACCCAACCCCCCTGCCCAGCTGGCCAACTATGAA
  TTTGACGTCCTTATCTCGGCTGCAGGAGGTAAATTCGTCCCTGAAGGCTTCAAAGTTCGAGAAATGCGAG
  GCAAACTGGCCATTGGCATCACAGCCAACTTTGTGAATGGACGCACCGTGGAGGAGACACAGGTGCCGGA
  GATCAGTGGTGTAGCCAGGATCTACAACCAGAGCTTCTTCCAGAGCCTTCTCAAAGCCACAGGCATTGAT
  CTGGAGAACATTGTGTACTACAAGGACGACACCCACTACTTTGTGATGACAGCCAAGAAGCAGTGCCTGC
  TGCGGCTGGGGGTGCTGCGCCAGGACTGGCCAGACACCAATCGGCTGCTGGGCAGTGCCAATGTGGTGCC
  CGAGGCTCTGCAGCGCTTTACCCGGGCAGCTGCTGACTTTGCCACCCATGGCAAGCTCGGGAAACTAGAG
  TTTGCCCAGGATGCCCATGGGCAGCCTGATGTCTCTGCCTTTGACTTCACGAGCATGATGCGGGCAGAGA
  GTTCTGCTCGTGTGCAAGAGAAGCATGGCGCCCGCCTGCTGCTGGGACTGGTGGGGGACTGCCTGGTGGA
  GCCCTTCTGGCCCCTGGGCACTGGAGTGGCACGGGGCTTCCTGGCAGCCTTTGATGCAGCCTGGATGGTG
  AAGCGGTGGGCAGAGGGCGCTGAGTCCCTAGAGGTGTTGGCTGAGCGTGAGAGCCTGTACCAGCTTCTGT
  CACAGACATCCCCAGAAAACATGCATCGCAATGTGGCCCAGTATGGGCTGGACCCAGCCACCCGCTACCC
  CAACCTGAACCTCCGGGCAGTGACCCCCAATCAGGTACGAGACCTGTATGATGTGCTAGCCAAGGAGCCT
  GTGCAGAGGAACAACGACAAGACAGATACAGGGATGCCAGCCACCGGGTCGGCAGGCACCCAGGAGGAGC
  TGCTACGCTGGTGCCAGGAGCAGACAGCTGGGTACCCGGGAGTCCACGTCTCCGATTTGTCTTCCTCCTG
  GGCTGATGGGCTAGCTCTGTGTGCCCTGGTGTACCGGCTGCAGCCTGGCCTGCTGGAACCCTCAGAGCTG
  CAGGGGCTGGGAGCTCTGGAAGCAACTGCTTGGGCACTAAAGGTGGCAGAGAATGAGCTGGGCATCACAC
  CGGTGGTGTCTGCACAGGCCGTGGTAGCAGGGAGTGACCCACTGGGCCTCATTGCCTACCTCAGCCACTT
  CCACAGTGCCTTCAAGAGCATGGCCCACAGCCCAGGCCCTGTCAGCCAGGCCTCCCCAGGGACCTCCAGT
  GCTGTATTATTCCTTAGTAAACTTCAGAGGACCCTGCAGCGATCCCGGGCCAAGGAAAATGCAGAGGATG
  CTGGTGGCAAGAAGCTGCGCTTGGAGATGGAGGCCGAGACCCCAAGTACTGAGGTGCCACCTGACCCAGA
  GCCTGGTGTACCCCTGACACCCCCATCCCAACACCAGGAGGCCGGTGCTGGGGACCTGTGTGCACTTTGT
  GGGGAACACCTCTATGTCCTGGAACGCCTCTGTGTCAACGGCCATTTCTTCCACCGGAGCTGCTTCCGCT
  GCCATACCTGTGAGGCCACACTGTGGCCAGGTGGCTACGAGCAGCACCCAGGAGATGGACATTTCTACTG
  CCTCCAGCACCTGCCCCAGACAGACCACAAAGCGGAAGGCAGCGATAGAGGCCCTGAGAGTCCGGAGCTC
  CCCACACCAAGTGAGAATAGCATGCCACCAGGCCTCTCAACTCCCACAGCCTCGCAGGAGGGGGCCGGTC
  CTGTTCCAGATCCCAGCCAGCCCACCCGTCGGCAGATCCGCCTCTCCAGCCCGGAGCGCCAGCGGTTGTC
  CTCCCTTAACCTTACCCCTGACCCGGAAATGGAGCCTCCACCCAAGCCTCCCCGCAGCTGCTCCGCCTTG
  GCCCGCCACGCCCTGGAGAGCAGCTTTGTGGGCTGGGGCCTGCCAGTCCAGAGCCCTCAAGCTCTTGTGG
  CCATGGAGAAGGAGGAAAAAGAGAGTCCCTTCTCCAGTGAAGAGGAAGAAGAAGATGTGCCTTTGGACTC
  AGATGTGGAACAGGCCCTGCAGACCTTTGCCAAGACCTCAGGCACCATGAATAACTACCCAACATGGCGT
  CGGACTCTGCTGCGCCGTGCGAAGGAGGAGGAGATGAAGAGGTTCTGCAAGGCCCAGACCATCCAACGGC
  GACTAAATGAGATTGAGGCTGCCTTGAGGGAGCTAGAGGCCGAGGGCGTGAAGCTGGAGCTGGCCTTGAG
  GCGCCAGAGCAGTTCCCCAGAACAGCAAAAGAAACTATGGGTAGGACAGCTGCTACAGCTCGTTGACAAG
  AAAAACAGCCTGGTGGCTGAGGAGGCCGAGCTCATGATCACGGTGCAGGAATTGAATCTGGAGGAGAAAC
  AGTGGCAGCTGGACCAGGAGCTACGAGGCTACATGAACCGGGAAGAAAACCTAAAGACAGCTGCTGATCG
  GCAGGCTGAGGACCAGGTCCTGAGGAAGCTGGTGGATTTGGTCAACCAGAGAGATGCCCTCATCCGCTTC
  CAGGAGGAGCGCAGGCTCAGCGAGCTGGCCTTGGGGACAGGGGCCCAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208308 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASPTSTNPAHAHFESFLQAQLCQDVLSSFQELCGALGLEPGGGLPQYHKIKDQLNYWSAKSLWTKLDKR
  AGQPVYQQGRACTSTKCLVVGAGPCGLRVAVELALLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGA
  KKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVTFTGLQPPPRKGSGWRAQLQPNPPAQLANYE
  FDVLISAAGGKFVPEGFKVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGID
  LENIVYYKDDTHYFVMTAKKQCLLRLGVLRQDWPDTNRLLGSANVVPEALQRFTRAAADFATHGKLGKLE
  FAQDAHGQPDVSAFDFTSMMRAESSARVQEKHGARLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMV
  KRWAEGAESLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVRDLYDVLAKEP
  VQRNNDKTDTGMPATGSAGTQEELLRWCQEQTAGYPGVHVSDLSSSWADGLALCALVYRLQPGLLEPSEL
  QGLGALEATAWALKVAENELGITPVVSAQAVVAGSDPLGLIAYLSHFHSAFKSMAHSPGPVSQASPGTSS
  AVLFLSKLQRTLQRSRAKENAEDAGGKKLRLEMEAETPSTEVPPDPEPGVPLTPPSQHQEAGAGDLCALC
  GEHLYVLERLCVNGHFFHRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESPEL
  PTPSENSMPPGLSTPTASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNLTPDPEMEPPPKPPRSCSAL
  ARHALESSFVGWGLPVQSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQALQTFAKTSGTMNNYPTWR
  RTLLRRAKEEEMKRFCKAQTIQRRLNEIEAALRELEAEGVKLELALRRQSSSPEQQKKLWVGQLLQLVDK
  KNSLVAEEAELMITVQELNLEEKQWQLDQELRGYMNREENLKTAADRQAEDQVLRKLVDLVNQRDALIRF
  QEERRLSELALGTGAQG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022765
• ORF Size: 3201 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022765.4
• RefSeq Size: 3661 bp
• RefSeq ORF: 3204 bp
• Locus ID: 64780
• UniProt ID: Q8TDZ2
• Cytogenetics: 6q21
• Domains: CH, LIM
• MW: 117.9 kDa
• Gene Summary: This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]