MICAL1 (NM_022765) Human Tagged ORF Clone

CAT#: RC208308

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MICAL1 (Myc-DDK-tagged)-Human microtubule associated monoxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1



  "NM_022765" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 997.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MICAL1
Synonyms MICAL; MICAL-1; NICAL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208308 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCACCTACCTCCACCAACCCAGCGCATGCCCACTTTGAGAGCTTCCTGCAGGCCCAGCTGTGCC
AGGACGTGCTGAGCAGCTTCCAGGAGCTGTGTGGGGCCCTGGGGCTGGAACCCGGTGGGGGGCTGCCCCA
GTACCACAAGATCAAGGACCAGCTCAACTACTGGAGCGCCAAGTCACTGTGGACCAAGCTGGACAAGCGA
GCAGGCCAGCCTGTCTACCAGCAGGGCCGGGCCTGCACCAGCACCAAGTGCCTGGTGGTGGGTGCTGGAC
CTTGCGGGCTGCGGGTCGCTGTGGAGCTGGCGCTGCTGGGGGCCCGAGTGGTGCTGGTGGAAAAGCGCAC
CAAGTTCTCTCGCCACAACGTGCTCCACCTCTGGCCCTTCACCATCCACGACCTGCGGGCACTCGGTGCT
AAGAAGTTCTACGGGCGCTTCTGCACCGGCACCCTGGACCACATCAGCATCAGGCAGCTCCAGCTGCTTC
TGCTGAAGGTAGCATTGCTGCTGGGGGTGGAAATTCACTGGGGTGTCACTTTCACTGGCCTCCAGCCCCC
TCCTAGGAAGGGGAGTGGCTGGCGTGCCCAGCTCCAACCCAACCCCCCTGCCCAGCTGGCCAACTATGAA
TTTGACGTCCTTATCTCGGCTGCAGGAGGTAAATTCGTCCCTGAAGGCTTCAAAGTTCGAGAAATGCGAG
GCAAACTGGCCATTGGCATCACAGCCAACTTTGTGAATGGACGCACCGTGGAGGAGACACAGGTGCCGGA
GATCAGTGGTGTAGCCAGGATCTACAACCAGAGCTTCTTCCAGAGCCTTCTCAAAGCCACAGGCATTGAT
CTGGAGAACATTGTGTACTACAAGGACGACACCCACTACTTTGTGATGACAGCCAAGAAGCAGTGCCTGC
TGCGGCTGGGGGTGCTGCGCCAGGACTGGCCAGACACCAATCGGCTGCTGGGCAGTGCCAATGTGGTGCC
CGAGGCTCTGCAGCGCTTTACCCGGGCAGCTGCTGACTTTGCCACCCATGGCAAGCTCGGGAAACTAGAG
TTTGCCCAGGATGCCCATGGGCAGCCTGATGTCTCTGCCTTTGACTTCACGAGCATGATGCGGGCAGAGA
GTTCTGCTCGTGTGCAAGAGAAGCATGGCGCCCGCCTGCTGCTGGGACTGGTGGGGGACTGCCTGGTGGA
GCCCTTCTGGCCCCTGGGCACTGGAGTGGCACGGGGCTTCCTGGCAGCCTTTGATGCAGCCTGGATGGTG
AAGCGGTGGGCAGAGGGCGCTGAGTCCCTAGAGGTGTTGGCTGAGCGTGAGAGCCTGTACCAGCTTCTGT
CACAGACATCCCCAGAAAACATGCATCGCAATGTGGCCCAGTATGGGCTGGACCCAGCCACCCGCTACCC
CAACCTGAACCTCCGGGCAGTGACCCCCAATCAGGTACGAGACCTGTATGATGTGCTAGCCAAGGAGCCT
GTGCAGAGGAACAACGACAAGACAGATACAGGGATGCCAGCCACCGGGTCGGCAGGCACCCAGGAGGAGC
TGCTACGCTGGTGCCAGGAGCAGACAGCTGGGTACCCGGGAGTCCACGTCTCCGATTTGTCTTCCTCCTG
GGCTGATGGGCTAGCTCTGTGTGCCCTGGTGTACCGGCTGCAGCCTGGCCTGCTGGAACCCTCAGAGCTG
CAGGGGCTGGGAGCTCTGGAAGCAACTGCTTGGGCACTAAAGGTGGCAGAGAATGAGCTGGGCATCACAC
CGGTGGTGTCTGCACAGGCCGTGGTAGCAGGGAGTGACCCACTGGGCCTCATTGCCTACCTCAGCCACTT
CCACAGTGCCTTCAAGAGCATGGCCCACAGCCCAGGCCCTGTCAGCCAGGCCTCCCCAGGGACCTCCAGT
GCTGTATTATTCCTTAGTAAACTTCAGAGGACCCTGCAGCGATCCCGGGCCAAGGAAAATGCAGAGGATG
CTGGTGGCAAGAAGCTGCGCTTGGAGATGGAGGCCGAGACCCCAAGTACTGAGGTGCCACCTGACCCAGA
GCCTGGTGTACCCCTGACACCCCCATCCCAACACCAGGAGGCCGGTGCTGGGGACCTGTGTGCACTTTGT
GGGGAACACCTCTATGTCCTGGAACGCCTCTGTGTCAACGGCCATTTCTTCCACCGGAGCTGCTTCCGCT
GCCATACCTGTGAGGCCACACTGTGGCCAGGTGGCTACGAGCAGCACCCAGGAGATGGACATTTCTACTG
CCTCCAGCACCTGCCCCAGACAGACCACAAAGCGGAAGGCAGCGATAGAGGCCCTGAGAGTCCGGAGCTC
CCCACACCAAGTGAGAATAGCATGCCACCAGGCCTCTCAACTCCCACAGCCTCGCAGGAGGGGGCCGGTC
CTGTTCCAGATCCCAGCCAGCCCACCCGTCGGCAGATCCGCCTCTCCAGCCCGGAGCGCCAGCGGTTGTC
CTCCCTTAACCTTACCCCTGACCCGGAAATGGAGCCTCCACCCAAGCCTCCCCGCAGCTGCTCCGCCTTG
GCCCGCCACGCCCTGGAGAGCAGCTTTGTGGGCTGGGGCCTGCCAGTCCAGAGCCCTCAAGCTCTTGTGG
CCATGGAGAAGGAGGAAAAAGAGAGTCCCTTCTCCAGTGAAGAGGAAGAAGAAGATGTGCCTTTGGACTC
AGATGTGGAACAGGCCCTGCAGACCTTTGCCAAGACCTCAGGCACCATGAATAACTACCCAACATGGCGT
CGGACTCTGCTGCGCCGTGCGAAGGAGGAGGAGATGAAGAGGTTCTGCAAGGCCCAGACCATCCAACGGC
GACTAAATGAGATTGAGGCTGCCTTGAGGGAGCTAGAGGCCGAGGGCGTGAAGCTGGAGCTGGCCTTGAG
GCGCCAGAGCAGTTCCCCAGAACAGCAAAAGAAACTATGGGTAGGACAGCTGCTACAGCTCGTTGACAAG
AAAAACAGCCTGGTGGCTGAGGAGGCCGAGCTCATGATCACGGTGCAGGAATTGAATCTGGAGGAGAAAC
AGTGGCAGCTGGACCAGGAGCTACGAGGCTACATGAACCGGGAAGAAAACCTAAAGACAGCTGCTGATCG
GCAGGCTGAGGACCAGGTCCTGAGGAAGCTGGTGGATTTGGTCAACCAGAGAGATGCCCTCATCCGCTTC
CAGGAGGAGCGCAGGCTCAGCGAGCTGGCCTTGGGGACAGGGGCCCAGGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208308 protein sequence
Red=Cloning site Green=Tags(s)

MASPTSTNPAHAHFESFLQAQLCQDVLSSFQELCGALGLEPGGGLPQYHKIKDQLNYWSAKSLWTKLDKR
AGQPVYQQGRACTSTKCLVVGAGPCGLRVAVELALLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGA
KKFYGRFCTGTLDHISIRQLQLLLLKVALLLGVEIHWGVTFTGLQPPPRKGSGWRAQLQPNPPAQLANYE
FDVLISAAGGKFVPEGFKVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGID
LENIVYYKDDTHYFVMTAKKQCLLRLGVLRQDWPDTNRLLGSANVVPEALQRFTRAAADFATHGKLGKLE
FAQDAHGQPDVSAFDFTSMMRAESSARVQEKHGARLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMV
KRWAEGAESLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVRDLYDVLAKEP
VQRNNDKTDTGMPATGSAGTQEELLRWCQEQTAGYPGVHVSDLSSSWADGLALCALVYRLQPGLLEPSEL
QGLGALEATAWALKVAENELGITPVVSAQAVVAGSDPLGLIAYLSHFHSAFKSMAHSPGPVSQASPGTSS
AVLFLSKLQRTLQRSRAKENAEDAGGKKLRLEMEAETPSTEVPPDPEPGVPLTPPSQHQEAGAGDLCALC
GEHLYVLERLCVNGHFFHRSCFRCHTCEATLWPGGYEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESPEL
PTPSENSMPPGLSTPTASQEGAGPVPDPSQPTRRQIRLSSPERQRLSSLNLTPDPEMEPPPKPPRSCSAL
ARHALESSFVGWGLPVQSPQALVAMEKEEKESPFSSEEEEEDVPLDSDVEQALQTFAKTSGTMNNYPTWR
RTLLRRAKEEEMKRFCKAQTIQRRLNEIEAALRELEAEGVKLELALRRQSSSPEQQKKLWVGQLLQLVDK
KNSLVAEEAELMITVQELNLEEKQWQLDQELRGYMNREENLKTAADRQAEDQVLRKLVDLVNQRDALIRF
QEERRLSELALGTGAQG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022765
ORF Size 3201 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022765.4
RefSeq Size 3661 bp
RefSeq ORF 3204 bp
Locus ID 64780
UniProt ID Q8TDZ2
Cytogenetics 6q21
Domains CH, LIM
MW 117.9 kDa
Gene Summary This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Other Versions

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