FBXO25 (NM_183421) Human Tagged ORF Clone

CAT#: RC208287

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FBXO25 (Myc-DDK-tagged)-Human F-box protein 25 (FBXO25), transcript variant 1



  "NM_183421" in other vectors (6)

Special Offer: 20% off on control vector. Use code "Shuttle20".

USD 477.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FBXO25
Synonyms FBX25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208287 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATTTTTGGGTCAGGACTGGAGATCTCCTGGATGGAGTTGGATTAAGACAGAAGATGGCTGGAAGA
GATGTGAATCTTGTAGTCAGAAACTTGAAAGAGAGAATAACCATTGTAACATCAGTCACAGCATTATCTT
AAATAGTGAAGATGGAGAAATATTCAATAATGAAGAGCATGAATATGCATCGAAAAAAAGGAAAAAGGAC
CATTTTAGAAATGACACAAATACTCAAAGTTTTTATCGTGAAAAATGGATCTATGTCCATAAAGAAAGCA
CAAAGGAAAGGCATGGCTATTGCACCTTGGGAGAAGCCTTTAATCGGTTAGACTTCTCAAGTGCAATTCA
AGATATCCGAAGGTTCAATTATGTGGTCAAACTGTTGCAGCTAATTGCAAAATCCCAGTTAACTTCATTG
AGTGGCGTGGCACAGAAGAATTACTTCAACATTTTGGATAAAATCGTTCAAAAGGTTCTTGATGACCACC
ACAATCCTCGCTTAATCAAAGATCTTCTGCAAGACCTAAGCTCTACCCTCTGCATTCTTATTAGAGGAGT
AGGGAAGTCTGTATTAGTGGGAAACATCAATATTTGGATTTGCCGATTAGAAACTATTCTCGCCTGGCAA
CAACAGCTACAGGATCTTCAGATGACTAAGCAAGTGAACAATGGCCTCACCCTCAGTGACCTTCCTCTGC
ACATGCTGAACAACATCCTATACCGGTTCTCAGACGGATGGGACATCATCACCTTAGGCCAGGTGACCCC
CACGTTGTATATGCTTAGTGAAGACAGACAGCTGTGGAAGAAGCTTTGTCAGTACCATTTTGCTGAAAAG
CAGTTTTGTAGACATTTGATCCTTTCAGAAAAAGGTCATATTGAATGGAAGTTGATGTACTTTGCACTTC
AGAAACATTACCCAGCGAAGGAGCAGTACGGAGACACACTGCATTTCTGTCGGCACTGCAGCATTCTCTT
TTGGAAGGACTACCATCTTGCTTTACTATTCAAGGACTCAGGACACCCCTGCACGGCGGCCGACCCTGAC
AGCTGCTTCACGCCTGTGTCTCCGCAGCACTTCATCGACCTCTTCAAGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208287 protein sequence
Red=Cloning site Green=Tags(s)

MPFLGQDWRSPGWSWIKTEDGWKRCESCSQKLERENNHCNISHSIILNSEDGEIFNNEEHEYASKKRKKD
HFRNDTNTQSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFNYVVKLLQLIAKSQLTSL
SGVAQKNYFNILDKIVQKVLDDHHNPRLIKDLLQDLSSTLCILIRGVGKSVLVGNINIWICRLETILAWQ
QQLQDLQMTKQVNNGLTLSDLPLHMLNNILYRFSDGWDIITLGQVTPTLYMLSEDRQLWKKLCQYHFAEK
QFCRHLILSEKGHIEWKLMYFALQKHYPAKEQYGDTLHFCRHCSILFWKDYHLALLFKDSGHPCTAADPD
SCFTPVSPQHFIDLFKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_183421
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_183421.1, NP_904357.1
RefSeq Size 2529 bp
RefSeq ORF 1104 bp
Locus ID 26260
UniProt ID Q8TCJ0
Cytogenetics 8p23.3
MW 43.3 kDa
Gene Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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