FBXO25 (NM_183421) Human Tagged ORF Clone

CAT#: RC208287

FBXO25 (Myc-DDK-tagged)-Human F-box protein 25 (FBXO25), transcript variant 1

Clone Modification / Custom Cloning Service

USD 577.00


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Size
    • 10 ug

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Specifications

Product Data
Product Name FBXO25 (NM_183421) Human Tagged ORF Clone
Symbol FBXO25
Synonyms FBX25; FBX25
Vector pCMV6-Entry
Sequence Data
>RC208287 representing NM_183421
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATTTTTGGGTCAGGACTGGAGATCTCCTGGATGGAGTTGGATTAAGACAGAAGATGGCTGGAAGA
GATGTGAATCTTGTAGTCAGAAACTTGAAAGAGAGAATAACCATTGTAACATCAGTCACAGCATTATCTT
AAATAGTGAAGATGGAGAAATATTCAATAATGAAGAGCATGAATATGCATCGAAAAAAAGGAAAAAGGAC
CATTTTAGAAATGACACAAATACTCAAAGTTTTTATCGTGAAAAATGGATCTATGTCCATAAAGAAAGCA
CAAAGGAAAGGCATGGCTATTGCACCTTGGGAGAAGCCTTTAATCGGTTAGACTTCTCAAGTGCAATTCA
AGATATCCGAAGGTTCAATTATGTGGTCAAACTGTTGCAGCTAATTGCAAAATCCCAGTTAACTTCATTG
AGTGGCGTGGCACAGAAGAATTACTTCAACATTTTGGATAAAATCGTTCAAAAGGTTCTTGATGACCACC
ACAATCCTCGCTTAATCAAAGATCTTCTGCAAGACCTAAGCTCTACCCTCTGCATTCTTATTAGAGGAGT
AGGGAAGTCTGTATTAGTGGGAAACATCAATATTTGGATTTGCCGATTAGAAACTATTCTCGCCTGGCAA
CAACAGCTACAGGATCTTCAGATGACTAAGCAAGTGAACAATGGCCTCACCCTCAGTGACCTTCCTCTGC
ACATGCTGAACAACATCCTATACCGGTTCTCAGACGGATGGGACATCATCACCTTAGGCCAGGTGACCCC
CACGTTGTATATGCTTAGTGAAGACAGACAGCTGTGGAAGAAGCTTTGTCAGTACCATTTTGCTGAAAAG
CAGTTTTGTAGACATTTGATCCTTTCAGAAAAAGGTCATATTGAATGGAAGTTGATGTACTTTGCACTTC
AGAAACATTACCCAGCGAAGGAGCAGTACGGAGACACACTGCATTTCTGTCGGCACTGCAGCATTCTCTT
TTGGAAGGACTACCATCTTGCTTTACTATTCAAGGACTCAGGACACCCCTGCACGGCGGCCGACCCTGAC
AGCTGCTTCACGCCTGTGTCTCCGCAGCACTTCATCGACCTCTTCAAGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208287 representing NM_183421
Red=Cloning site Green=Tags(s)

MPFLGQDWRSPGWSWIKTEDGWKRCESCSQKLERENNHCNISHSIILNSEDGEIFNNEEHEYASKKRKKD
HFRNDTNTQSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFNYVVKLLQLIAKSQLTSL
SGVAQKNYFNILDKIVQKVLDDHHNPRLIKDLLQDLSSTLCILIRGVGKSVLVGNINIWICRLETILAWQ
QQLQDLQMTKQVNNGLTLSDLPLHMLNNILYRFSDGWDIITLGQVTPTLYMLSEDRQLWKKLCQYHFAEK
QFCRHLILSEKGHIEWKLMYFALQKHYPAKEQYGDTLHFCRHCSILFWKDYHLALLFKDSGHPCTAADPD
SCFTPVSPQHFIDLFKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_183421
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_183421.1, NP_904357
RefSeq Size 2529
RefSeq ORF 1104
Locus ID 26260
Cytogenetics 8p23.3
MW 43.1 kDa
Gene Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
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Citations (1)