TMEM17 (NM_198276) Human Tagged ORF Clone

CAT#: RC208232

TMEM17 (Myc-DDK-tagged)-Human transmembrane protein 17 (TMEM17)

Product Images

Circular map for RC208232

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  Western blot validation of overexpression lysate (Cat# LY405002) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208232 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TMEM17 protein (Cat# TP308232). The protein was produced from HEK293T cells transfected with TMEM17 cDNA clone (Cat# RC208232) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208232 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCCAGATCCGGTGCGCCAGCGGCTGGGAAACTTCAGCCGGGCCGTGTTCAGTGATTCCAATC
  GGACCAGTCCAGAGTCCAATGAGGGTCCGGAAAATGAAATGGTCTCCAGTTTGGCACTGCAGATGTCACT
  TTATTTTAATACCTACTATTTCCCACTGTGGTGGGTGAGCAGCATTATGATGCTTCACATGAAGTATTCA
  ATCTTGCCTGACTACTACAAATTCATTGTGATCACTGTTATCATCCTAATAACCTTAATTGAAGCCATCC
  GGTTGTATCTGGGCTACGTGGGTAACCTACAGGAGAAGGTTCCTGAGTTGGCTGGCTTTTGGCTTTTGAG
  CCTTCTATTGCAGTTACCTTTAATTCTTTTCTTGCTCTTTAATGAAGGCCTAACAAATCTGCCCTTGGAA
  AAAGCGATACATATCATCTTCACTCTCTTCCTTGCTTTCCAAGTTGTTGCAGCATTTCTTACCTTAAGGA
  AAATGGTTAATCAGTTGGCAGTTCGTTTCCACCTCCAAGACTTTGACCGGCTCTCTGCAAACAGAGGAGA
  CATGAGAAGGATGAGGTCATGTATAGAAGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208232 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELPDPVRQRLGNFSRAVFSDSNRTSPESNEGPENEMVSSLALQMSLYFNTYYFPLWWVSSIMMLHMKYS
  ILPDYYKFIVITVIILITLIEAIRLYLGYVGNLQEKVPELAGFWLLSLLLQLPLILFLLFNEGLTNLPLE
  KAIHIIFTLFLAFQVVAAFLTLRKMVNQLAVRFHLQDFDRLSANRGDMRRMRSCIEEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198276
• ORF Size: 594 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198276.1, NP_938017.1
• RefSeq Size: 1925 bp
• RefSeq ORF: 597 bp
• Locus ID: 200728
• UniProt ID: Q86X19
• Cytogenetics: 2p15
• Protein Families: Transmembrane
• MW: 23.1 kDa
• Gene Summary: Transmembrane component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling (By similarity).[UniProtKB/Swiss-Prot Function]