DHX29 (NM_019030) Human Tagged ORF Clone

CAT#: RC208227

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DHX29 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 29 (DHX29)



  "NM_019030" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,632.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DHX29
Synonyms DDX29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208227 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGGCAAGAACAAGAAACACAAGGCTCCAGCGGCCGCGGTGGTCCGGGCCGCCGTGTCTGCTTCCA
GAGCCAAATCTGCCGAGGCTGGAATTGCCGGGGAGGCCCAAAGCAAGAAGCCAGTGTCCAGGCCGGCCAC
CGCTGCCGCTGCCGCTGCCGGCTCCAGGGAGCCCCGTGTCAAGCAAGGTCCAAAAATTTATAGTTTTAAT
TCTACAAATGATTCTAGTGGTCCTGCAAATCTGGATAAATCTATTTTGAAAGTGGTAATTAATAACAAAC
TAGAGCAAAGAATTATTGGAGTGATCAATGAGCATAAAAAGCAAAATAATGACAAAGGAATGATTTCTGG
AAGACTTACTGCCAAAAAATTGCAGGATTTATACATGGCTTTACAAGCATTTTCATTTAAGACAAAGGAC
ATTGAAGATGCCATGACCAATACACTCTTATATGGAGGTGACCTTCATTCTGCCTTGGATTGGCTCTGTT
TAAACCTTTCAGATGATGCACTTCCTGAAGGATTCAGTCAGGAATTTGAAGAGCAGCAACCTAAAAGTAG
GCCTAAATTTCAGTCTCCTCAAATACAAGCCACTATTTCACCTCCATTGCAACCTAAAACAAAAACATAT
GAAGAGGACCCTAAGAGTAAGCCAAAAAAGGAAGAAAAAAATATGGAAGTAAATATGAAAGAGTGGATTT
TACGATATGCTGAACAACAAAATGAAGAAGAGAAGAATGAGAATTCTAAAAGTTTAGAAGAGGAGGAAAA
ATTTGACCCTAATGAAAGGTACTTACATCTTGCAGCAAAACTGCTGGATGCAAAAGAACAAGCAGCTACC
TTTAAACTAGAAAAAAACAAGCAAGGCCAAAAAGAGGCTCAGGAAAAAATAAGGAAATTTCAAAGAGAAA
TGGAAACTTTAGAAGACCATCCAGTATTTAACCCAGCCATGAAGATTTCACATCAACAAAATGAAAGGAA
AAAGCCTCCTGTAGCCACAGAAGGAGAAAGTGCATTGAATTTTAATTTATTTGAAAAATCTGCAGCTGCT
ACTGAAGAAGAGAAAGATAAAAAGAAAGAACCTCATGATGTAAGAAATTTTGACTATACTGCTCGAAGTT
GGACTGGAAAATCTCCCAAACAATTTCTGATTGATTGGGTCAGGAAGAATCTTCCCAAGAGTCCAAATCC
TTCCTTTGAAAAAGTTCCAGTAGGTAGATACTGGAAATGTAGGGTTAGGGTAATCAAGTCTGAAGATGAT
GTCCTGGTAGTATGCCCTACAATCTTAACAGAAGATGGCATGCAAGCTCAGCACCTGGGAGCTACTTTAG
CCCTTTACCGTTTAGTTAAAGGGCAGTCAGTTCATCAGTTACTTCCTCCCACTTACCGAGATGTTTGGCT
GGAGTGGAGTGATGCAGAAAAGAAAAGGGAAGAATTAAATAAAATGGAAACCAATAAACCACGTGATCTT
TTTATTGCCAAACTTCTGAATAAACTGAAACAGCAGCAACAGCAGCAACAACAGCATTCTGAAAATAAGA
GAGAAAACTCTGAAGATCCCGAGGAATCTTGGGAAAATTTAGTTTCGGATGAGGATTTTTCTGCACTGTC
CTTGGAATCAGCAAATGTGGAAGATTTGGAACCTGTTAGAAACCTCTTTAGAAAGTTGCAAAGCACACCT
AAGTATCAGAAACTTCTAAAGGAAAGACAACAGCTACCTGTATTTAAACATCGGGACTCAATTGTTGAAA
CTCTTAAAAGGCATCGGGTAGTGGTTGTGGCAGGTGAAACAGGGAGTGGTAAAAGTACTCAGGTACCACA
TTTTCTATTGGAAGATTTGCTTCTAAATGAGTGGGAAGCAAGTAAATGTAACATTGTCTGTACCCAACAC
CGAAGAATCTCAGCAGTTAGTTTAGCCAACAGAGTATGTGATGAATTGGGCTGTGAAAATGGACCTGGAG
GAAGGAATTCCTTGTGTGGATATCAGATCCGGATGGAATCTCGAGCTTGTGAATCTACCAGGTTACTCTA
TTGTACAACAGGGGTTTTGCTAAGGAAACTTCAAGAAGATGGTCTTCTAAGTAATGTGTCTCATGTTATT
GTAGATGAGGTTCATGAAAGAAGTGTCCAGTCAGACTTCCTACTAATTATCTTGAAGGAAATTTTACAGA
AACGTTCTGATCTACACTTGATTCTAATGAGTGCCACTGTGGACAGCGAAAAATTTTCTACATATTTCAC
ACACTGCCCCATTCTCAGAATTTCAGGAAGGAGTTATCCTGTTGAGGTTTTTCATCTTGAAGATATAATA
GAAGAAACAGGCTTTGTACTGGAAAAAGACTCAGAATATTGTCAGAAATTTCTGGAAGAGGAAGAAGAAG
TAACCATTAATGTTACAAGCAAAGCAGGGGGAATAAAAAAATATCAGGAATACATCCCAGTTCAGACTGG
AGCACATGCTGATTTAAATCCATTTTACCAAAAGTACAGCAGCCGCACTCAGCATGCTATTCTATACATG
AATCCTCATAAAATCAACCTGGATCTCATTTTGGAACTTCTTGCATACTTAGATAAAAGTCCCCAATTCA
GAAATATTGAAGGAGCAGTATTGATCTTTTTACCAGGACTTGCTCATATTCAGCAGTTGTATGATCTTCT
ATCAAATGATAGAAGATTTTATTCTGAACGATATAAAGTGATAGCTCTGCATTCTATTCTTTCAACCCAA
GATCAAGCTGCAGCATTCACACTTCCCCCTCCAGGAGTCAGGAAGATTGTTTTAGCAACCAATATTGCAG
AGACGGGTATCACTATTCCTGATGTTGTATTTGTAATTGATACTGGAAGAACAAAAGAAAATAAGTACCA
TGAAAGCAGTCAGATGAGTTCTTTGGTGGAGACGTTTGTCAGTAAAGCCAGTGCTTTGCAGCGCCAGGGA
AGAGCTGGGCGGGTCAGAGATGGCTTCTGTTTCCGAATGTACACAAGAGAAAGATTTGAAGGCTTTATGG
ATTATTCTGTTCCTGAAATCTTACGTGTACCTTTGGAGGAATTATGCCTTCATATTATGAAATGTAATCT
TGGTTCTCCTGAAGATTTCCTCTCCAAAGCCTTAGATCCTCCTCAGCTCCAAGTGATCAGCAATGCAATG
AATTTGCTCCGAAAAATTGGAGCTTGTGAATTAAATGAGCCTAAACTGACTCCGTTGGGCCAACACCTTG
CAGCTTTACCTGTGAATGTCAAGATTGGCAAGATGCTTATTTTTGGTGCCATATTTGGCTGCCTTGACCC
AGTGGCAACACTAGCTGCAGTTATGACAGAGAAGTCTCCTTTTACCACACCAATTGGTCGAAAAGATGAA
GCAGATCTTGCAAAATCAGCTTTGGCCATGGCGGATTCAGACCACCTGACGATCTACAATGCATATCTAG
GATGGAAGAAAGCACGACAAGAAGGAGGTTATCGTTCTGAAATCACATACTGCCGGAGGAACTTTCTTAA
TAGAACATCACTGTTAACCCTAGAGGATGTAAAGCAGGAGTTAATAAAGTTGGTTAAGGCAGCAGGATTT
TCATCTTCCACAACTTCTACCAGCTGGGAAGGAAACAGAGCCTCACAGACCCTCTCATTCCAAGAAATTG
CCCTTCTTAAAGCTGTACTGGTGGCTGGACTGTATGACAATGTGGGGAAGATAATCTATACAAAGTCAGT
GGATGTTACAGAAAAATTGGCTTGCATTGTGGAGACGGCCCAAGGCAAAGCACAAGTACACCCATCCTCA
GTAAATCGAGATTTGCAAACTCATGGATGGCTCTTATACCAGGAGAAGATAAGGTATGCCAGAGTGTATT
TGAGAGAAACTACCCTAATAACCCCTTTTCCAGTTTTACTTTTTGGTGGTGATATAGAAGTTCAGCACCG
AGAACGTCTTCTTTCTATTGATGGCTGGATCTATTTTCAGGCCCCTGTAAAGATAGCTGTCATTTTCAAG
CAGCTGAGAGTTCTCATTGATTCAGTTTTAAGAAAAAAGCTTGAAAATCCAAAGATGTCCCTTGAAAATG
ACAAGATTCTGCAGATCATTACGGAATTGATAAAAACAGAGAATAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208227 protein sequence
Red=Cloning site Green=Tags(s)

MGGKNKKHKAPAAAVVRAAVSASRAKSAEAGIAGEAQSKKPVSRPATAAAAAAGSREPRVKQGPKIYSFN
STNDSSGPANLDKSILKVVINNKLEQRIIGVINEHKKQNNDKGMISGRLTAKKLQDLYMALQAFSFKTKD
IEDAMTNTLLYGGDLHSALDWLCLNLSDDALPEGFSQEFEEQQPKSRPKFQSPQIQATISPPLQPKTKTY
EEDPKSKPKKEEKNMEVNMKEWILRYAEQQNEEEKNENSKSLEEEEKFDPNERYLHLAAKLLDAKEQAAT
FKLEKNKQGQKEAQEKIRKFQREMETLEDHPVFNPAMKISHQQNERKKPPVATEGESALNFNLFEKSAAA
TEEEKDKKKEPHDVRNFDYTARSWTGKSPKQFLIDWVRKNLPKSPNPSFEKVPVGRYWKCRVRVIKSEDD
VLVVCPTILTEDGMQAQHLGATLALYRLVKGQSVHQLLPPTYRDVWLEWSDAEKKREELNKMETNKPRDL
FIAKLLNKLKQQQQQQQQHSENKRENSEDPEESWENLVSDEDFSALSLESANVEDLEPVRNLFRKLQSTP
KYQKLLKERQQLPVFKHRDSIVETLKRHRVVVVAGETGSGKSTQVPHFLLEDLLLNEWEASKCNIVCTQH
RRISAVSLANRVCDELGCENGPGGRNSLCGYQIRMESRACESTRLLYCTTGVLLRKLQEDGLLSNVSHVI
VDEVHERSVQSDFLLIILKEILQKRSDLHLILMSATVDSEKFSTYFTHCPILRISGRSYPVEVFHLEDII
EETGFVLEKDSEYCQKFLEEEEEVTINVTSKAGGIKKYQEYIPVQTGAHADLNPFYQKYSSRTQHAILYM
NPHKINLDLILELLAYLDKSPQFRNIEGAVLIFLPGLAHIQQLYDLLSNDRRFYSERYKVIALHSILSTQ
DQAAAFTLPPPGVRKIVLATNIAETGITIPDVVFVIDTGRTKENKYHESSQMSSLVETFVSKASALQRQG
RAGRVRDGFCFRMYTRERFEGFMDYSVPEILRVPLEELCLHIMKCNLGSPEDFLSKALDPPQLQVISNAM
NLLRKIGACELNEPKLTPLGQHLAALPVNVKIGKMLIFGAIFGCLDPVATLAAVMTEKSPFTTPIGRKDE
ADLAKSALAMADSDHLTIYNAYLGWKKARQEGGYRSEITYCRRNFLNRTSLLTLEDVKQELIKLVKAAGF
SSSTTSTSWEGNRASQTLSFQEIALLKAVLVAGLYDNVGKIIYTKSVDVTEKLACIVETAQGKAQVHPSS
VNRDLQTHGWLLYQEKIRYARVYLRETTLITPFPVLLFGGDIEVQHRERLLSIDGWIYFQAPVKIAVIFK
QLRVLIDSVLRKKLENPKMSLENDKILQIITELIKTENN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019030
ORF Size 4107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019030.4
RefSeq Size 4491 bp
RefSeq ORF 4110 bp
Locus ID 54505
UniProt ID Q7Z478
Cytogenetics 5q11.2
MW 155.3 kDa
Gene Summary This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells. [provided by RefSeq, Sep 2016]

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