PALB2 (NM_024675) Human Tagged ORF Clone

CAT#: RC208189

PALB2 (Myc-DDK-tagged)-Human partner and localizer of BRCA2 (PALB2)

Product Images

Circular map for RC208189

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PALB2 (Cat# RC208189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PALB2 (Cat# TA806541)(1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PALB2
• Synonyms: FANCN; PNCA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208189 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGCCTCCCGGGAAGCCCCTCAGCTGTGAGGAGAAGGAAAAGTTAAAGGAGAAATTAGCATTCT
  TGAAAAGGGAATACAGCAAGACACTAGCCCGCCTTCAGCGTGCCCAAAGAGCTGAAAAGATTAAGCATTC
  TATTAAGAAAACAGTAGAAGAACAAGATTGTTTGTCTCAGCAGGATCTCTCACCGCAGCTAAAACACTCA
  GAACCTAAAAATAAAATATGTGTTTATGACAAGTTACACATCAAAACCCATCTTGATGAAGAAACTGGAG
  AAAAGACATCTATCACACTTGATGTTGGGCCTGAGTCCTTTAACCCTGGAGATGGCCCAGGAGGATTACC
  TATACAAAGAACAGATGACACCCAAGAACATTTTCCCCACAGGGTCAGTGACCCTAGTGGTGAGCAAAAG
  CAGAAGCTGCCAAGCAGAAGAAAGAAGCAGCAGAAGAGGACATTTATTTCACAGGAGAGAGACTGTGTCT
  TTGGCACTGATTCACTCAGATTGTCTGGGAAAAGACTAAAGGAACAGGAAGAAATCAGTAGCAAAAATCC
  TGCTAGATCACCAGTAACTGAAATAAGAACTCACCTTTTAAGTCTTAAATCTGAACTTCCAGATTCTCCA
  GAACCAGTTACAGAAATTAATGAAGACAGTGTATTAATTCCACCAACTGCCCAACCAGAAAAAGGTGTTG
  ATACATTCCTAAGAAGACCTAATTTCACCAGGGCGACTACAGTTCCTTTACAGACTCTATCAGATAGCGG
  TAGTAGTCAGCACCTTGAACACATTCCTCCTAAAGGTAGCAGTGAACTTACTACTCACGACCTAAAAAAC
  ATTAGATTTACTTCACCTGTAAGTTTGGAGGCACAAGGCAAAAAAATGACTGTCTCTACAGATAACCTCC
  TTGTAAATAAAGCTATAAGTAAAAGTGGCCAACTGCCCACAAGTTCTAATTTAGAGGCAAATATTTCATG
  TTCTCTAAATGAACTCACCTACAATAACTTACCAGCAAATGAAAACCAAAACTTAAAAGAACAAAATCAA
  ACAGAGAAATCTTTAAAATCTCCCAGTGACACTCTTGATGGCAGGAATGAAAATCTTCAGGAAAGTGAGA
  TTCTAAGTCAACCTAAGAGTCTTAGCCTGGAAGCAACCTCTCCTCTTTCTGCAGAAAAACATTCTTGCAC
  AGTGCCTGAAGGCCTTCTGTTTCCTGCAGAATATTATGTTAGAACAACACGAAGCATGTCCAATTGCCAG
  AGGAAAGTAGCCGTGGAGGCTGTCATTCAGAGTCATTTGGATGTCAAGAAAAAAGGGTTTAAAAATAAAA
  ATAAGGATGCAAGTAAAAATTTAAACCTTTCCAATGAGGAAACTGACCAAAGTGAAATTAGGATGTCTGG
  CACATGCACAGGACAACCAAGTTCAAGAACCTCTCAGAAACTTCTCTCATTAACTAAAGTCAGCTCTCCC
  GCTGGGCCCACTGAAGATAATGACTTGTCTAGGAAGGCAGTTGCCCAAGCACCTGGTAGAAGATACACAG
  GAAAAAGAAAATCAGCCTGCACCCCAGCATCAGATCATTGTGAACCACTTTTGCCAACTTCTAGCCTGTC
  GATTGTTAACAGGTCCAAGGAAGAAGTCACCTCACACAAATATCAGCACGAAAAATTATTTATTCAAGTG
  AAAGGGAAGAAAAGTCGTCATCAAAAAGAGGATTCCCTTTCTTGGAGTAATAGTGCTTATTTATCCTTGG
  ATGATGATGCTTTCACGGCTCCATTTCATAGGGATGGAATGCTGAGTTTAAAGCAACTACTGTCTTTTCT
  CAGTATCACAGACTTTCAGTTACCTGATGAAGACTTTGGACCTCTTAAGCTTGAAAAAGTGAAGTCCTGC
  TCAGAAAAACCAGTGGAGCCCTTTGAGTCAAAAATGTTTGGAGAGAGACATCTTAAAGAGGGAAGCTGTA
  TTTTTCCAGAGGAACTGAGTCCTAAACGCATGGATACAGAAATGGAGGACTTAGAAGAGGACCTTATTGT
  TCTACCAGGAAAATCACATCCCAAAAGGCCAAACTCGCAAAGCCAGCATACAAAGACGGGCCTTTCTTCA
  TCCATATTACTTTATACTCCTTTAAATACGGTTGCGCCTGATGATAATGACAGGCCTACCACAGACATGT
  GTTCACCTGCTTTCCCCATCTTAGGTACTACTCCAGCCTTTGGCCCTCAAGGCTCCTATGAAAAAGCATC
  TACAGAAGTTGCTGGACGAACTTGCTGCACACCCCAACTTGCTCATTTGAAAGACTCAGTCTGTCTTGCC
  AGTGATACTAAACAATTCGACAGTTCAGGCAGCCCAGCAAAACCACATACCACCCTGCAAGTGTCAGGCA
  GGCAAGGACAACCTACCTGTGACTGTGACTCTGTCCCGCCAGGAACACCTCCACCCATTGAGTCATTCAC
  TTTTAAAGAAAATCAGCTCTGTAGAAACACATGCCAGGAGCTGCATAAACATTCCGTCGAACAGACTGAA
  ACAGCAGAGCTTCCTGCTTCTGATAGCATAAACCCAGGCAACCTACAATTGGTTTCAGAGTTAAAGAATC
  CTTCAGGTTCCTGTTCCGTAGATGTGAGTGCCATGTTTTGGGAAAGAGCCGGTTGTAAAGAGCCATGTAT
  CATAACTGCTTGCGAAGATGTAGTTTCTCTTTGGAAAGCTCTGGATGCTTGGCAGTGGGAAAAACTTTAT
  ACCTGGCACTTCGCAGAGGTTCCAGTATTACAGATAGTTCCAGTGCCTGATGTGTATAATCTCGTGTGTG
  TAGCTTTGGGAAATTTGGAAATCAGAGAGATCAGGGCATTGTTTTGTTCCTCTGATGATGAAAGTGAAAA
  GCAAGTACTACTGAAGTCTGGAAATATAAAAGCTGTGCTTGGCCTGACAAAGAGGAGGCTAGTTAGTAGC
  AGTGGGACCCTTTCTGATCAACAAGTAGAAGTCATGACGTTTGCAGAAGATGGAGGAGGCAAAGAAAACC
  AATTTTTGATGCCCCCTGAGGAGACTATACTAACTTTTGCTGAGGTCCAAGGGATGCAAGAAGCTCTGCT
  TGGTACTACTATTATGAACAACATTGTTATTTGGAATTTAAAAACTGGTCAACTCCTGAAAAAGATGCAC
  ATTGATGATTCTTACCAAGCTTCAGTCTGTCACAAAGCCTATTCTGAAATGGGGCTTCTCTTTATTGTCC
  TGAGTCATCCCTGTGCCAAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAACCC
  TAAGACGACTCTCAGCGTGGGTGTGATGCTGTACTGTCTTCCTCCAGGGCAGGCTGGCAGGTTCCTGGAA
  GGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAACAATTGCCATTTGGGACTTACTTC
  TCGGTCAGTGTACTGCCCTCCTCCCACCTGTCTCTGACCAACATTGGTCTTTTGTGAAATGGTCGGGTAC
  AGACTCTCATTTGCTGGCTGGACAAAAAGATGGAAATATATTTGTATACCACTATTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208189 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDEPPGKPLSCEEKEKLKEKLAFLKREYSKTLARLQRAQRAEKIKHSIKKTVEEQDCLSQQDLSPQLKHS
  EPKNKICVYDKLHIKTHLDEETGEKTSITLDVGPESFNPGDGPGGLPIQRTDDTQEHFPHRVSDPSGEQK
  QKLPSRRKKQQKRTFISQERDCVFGTDSLRLSGKRLKEQEEISSKNPARSPVTEIRTHLLSLKSELPDSP
  EPVTEINEDSVLIPPTAQPEKGVDTFLRRPNFTRATTVPLQTLSDSGSSQHLEHIPPKGSSELTTHDLKN
  IRFTSPVSLEAQGKKMTVSTDNLLVNKAISKSGQLPTSSNLEANISCSLNELTYNNLPANENQNLKEQNQ
  TEKSLKSPSDTLDGRNENLQESEILSQPKSLSLEATSPLSAEKHSCTVPEGLLFPAEYYVRTTRSMSNCQ
  RKVAVEAVIQSHLDVKKKGFKNKNKDASKNLNLSNEETDQSEIRMSGTCTGQPSSRTSQKLLSLTKVSSP
  AGPTEDNDLSRKAVAQAPGRRYTGKRKSACTPASDHCEPLLPTSSLSIVNRSKEEVTSHKYQHEKLFIQV
  KGKKSRHQKEDSLSWSNSAYLSLDDDAFTAPFHRDGMLSLKQLLSFLSITDFQLPDEDFGPLKLEKVKSC
  SEKPVEPFESKMFGERHLKEGSCIFPEELSPKRMDTEMEDLEEDLIVLPGKSHPKRPNSQSQHTKTGLSS
  SILLYTPLNTVAPDDNDRPTTDMCSPAFPILGTTPAFGPQGSYEKASTEVAGRTCCTPQLAHLKDSVCLA
  SDTKQFDSSGSPAKPHTTLQVSGRQGQPTCDCDSVPPGTPPPIESFTFKENQLCRNTCQELHKHSVEQTE
  TAELPASDSINPGNLQLVSELKNPSGSCSVDVSAMFWERAGCKEPCIITACEDVVSLWKALDAWQWEKLY
  TWHFAEVPVLQIVPVPDVYNLVCVALGNLEIREIRALFCSSDDESEKQVLLKSGNIKAVLGLTKRRLVSS
  SGTLSDQQVEVMTFAEDGGGKENQFLMPPEETILTFAEVQGMQEALLGTTIMNNIVIWNLKTGQLLKKMH
  IDDSYQASVCHKAYSEMGLLFIVLSHPCAKESESLRSPVFQLIVINPKTTLSVGVMLYCLPPGQAGRFLE
  GDVKDHCAAAILTSGTIAIWDLLLGQCTALLPPVSDQHWSFVKWSGTDSHLLAGQKDGNIFVYHYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024675
• ORF Size: 3558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024675.4
• RefSeq Size: 4069 bp
• RefSeq ORF: 3561 bp
• Locus ID: 79728
• UniProt ID: Q86YC2
• Cytogenetics: 16p12.2
• MW: 131.3 kDa
• Gene Summary: This gene encodes a protein that may function in tumor suppression. This protein binds to and colocalizes with the breast cancer 2 early onset protein (BRCA2) in nuclear foci and likely permits the stable intranuclear localization and accumulation of BRCA2. [provided by RefSeq, Jul 2008]