MAVS (NM_020746) Human Tagged ORF Clone

CAT#: RC208175

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MAVS (Myc-DDK-tagged)-Human mitochondrial antiviral signaling protein (MAVS), nuclear gene encoding mitochondrial protein, transcript variant 1



  "NM_020746" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MAVS
Synonyms CARDIF; IPS-1; IPS1; VISA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208175 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTTTGCTGAAGACAAGACCTATAAGTATATCTGCCGCAATTTCAGCAATTTTTGCAATGTGGATG
TTGTAGAGATTCTGCCTTACCTGCCCTGCCTCACAGCAAGAGACCAGGATCGACTGCGGGCCACCTGCAC
ACTCTCAGGGAACCGGGACACCCTCTGGCATCTCTTCAATACCCTTCAGCGGCGGCCCGGCTGGGTGGAG
TACTTCATTGCGGCACTGAGGGGCTGTGAGCTAGTTGATCTCGCGGACGAAGTGGCCTCTGTCTACGAGA
GCTACCAGCCTCGGACCTCGGACCGTCCCCCAGACCCACTGGAGCCACCGTCACTTCCTGCTGAGAGGCC
AGGGCCCCCCACACCTGCTGCGGCCCACAGCATCCCCTACAACAGCTGCAGAGAGAAGGAGCCAAGTTAC
CCCATGCCTGTCCAGGAGACCCAGGCGCCAGAGTCCCCAGGAGAGAATTCAGAGCAAGCCCTGCAGACGC
TCAGCCCCAGAGCCATCCCAAGGAATCCAGATGGTGGCCCCCTGGAGTCCTCCTCTGACCTGGCAGCCCT
CAGCCCTCTGACCTCCAGCGGGCATCAGGAGAAGGACACAGAACTGGGCAGTACCCACACAGCAGGTGCG
ACCTCCAGCCTCACACCATCCCGTGGGCCTGTGTCTCCATCTGTCTCCTTCCAGCCCCTGGCCCGTTCCA
CCCCCAGGGCAAGCCGCTTGCCTGGACCCACAGGGTCAGTTGTATCTACTGGCACCTCCTTCTCCTCCTC
ATCCCCTGGCTTGGCCTCTGCAGGGGCTGCAGAGGGTAAACAGGGTGCAGAGAGTGACCAGGCCGAGCCT
ATCATCTGCTCCAGTGGGGCAGAGGCACCTGCCAACTCTCTGCCCTCCAAAGTGCCTACCACCTTGATGC
CTGTGAACACAGTGGCCCTGAAAGTGCCTGCCAACCCAGCATCTGTCAGCACAGTGCCCTCCAAGTTGCC
AACTAGCTCAAAGCCCCCTGGTGCAGTGCCTTCTAATGCGCTCACCAATCCAGCACCATCCAAATTGCCC
ATCAACTCAACCCGTGCTGGCATGGTGCCATCCAAAGTGCCTACTAGCATGGTGCTCACCAAGGTGTCTG
CCAGCACAGTCCCCACTGACGGGAGCAGCAGAAATGAGGAGACCCCAGCAGCTCCAACACCCGCCGGCGC
CACTGGAGGCAGCTCAGCCTGGCTAGACAGCAGCTTTGAGAATAGGGGCCTTGGGTCGGAGCTGAGTAAG
CCTGGCGTGCTGGCATCCCAGGTAGACAGCCCGTTCTCGGGCTGCTTCGAGGATCTTGCCATCAGTGCCA
GCACCTCCTTGGGCATGGGGCCCTGCCATGGCCCAGAGGAGAATGAGTATAAGTCCGAGGGCACCTTTGG
GATCCACGTGGCTGAGAACCCCAGCATCCAGCTCCTGGAGGGCAACCCTGGGCCACCTGCGGACCCGGAT
GGCGGCCCCAGGCCACAAGCCGACCGGAAGTTCCAGGAGAGGGAGGTGCCATGCCACAGGCCCTCACCTG
GGGCTCTGTGGCTCCAGGTGGCTGTGACAGGGGTGCTGGTAGTCACACTCCTGGTGGTGCTGTACCGGCG
GCGTCTGCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208175 protein sequence
Red=Cloning site Green=Tags(s)

MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVE
YFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSY
PMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGA
TSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEP
IICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLP
INSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSFENRGLGSELSK
PGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPD
GGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020746
ORF Size 1620 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020746.5
RefSeq Size 11771 bp
RefSeq ORF 1623 bp
Locus ID 57506
UniProt ID Q7Z434
Cytogenetics 20p13
Protein Families Transmembrane
Protein Pathways Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway
MW 56.6 kDa
Gene Summary This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral innate immunity. [provided by RefSeq, Jul 2020]

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