MAVS (NM_020746) Human Tagged ORF Clone

CAT#: RC208175

MAVS (Myc-DDK-tagged)-Human mitochondrial antiviral signaling protein (MAVS), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC208175

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAVS (Cat# RC208175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAVS (Cat# TA808253)(1:2000). Positive lysates LY412370 (100ug) and LC412370 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412370) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208175 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MAVS protein (Cat# TP308175). The protein was produced from HEK293T cells transfected with MAVS cDNA clone (Cat# RC208175) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAVS
• Synonyms: CARDIF; IPS-1; IPS1; VISA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208175 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTTGCTGAAGACAAGACCTATAAGTATATCTGCCGCAATTTCAGCAATTTTTGCAATGTGGATG
  TTGTAGAGATTCTGCCTTACCTGCCCTGCCTCACAGCAAGAGACCAGGATCGACTGCGGGCCACCTGCAC
  ACTCTCAGGGAACCGGGACACCCTCTGGCATCTCTTCAATACCCTTCAGCGGCGGCCCGGCTGGGTGGAG
  TACTTCATTGCGGCACTGAGGGGCTGTGAGCTAGTTGATCTCGCGGACGAAGTGGCCTCTGTCTACGAGA
  GCTACCAGCCTCGGACCTCGGACCGTCCCCCAGACCCACTGGAGCCACCGTCACTTCCTGCTGAGAGGCC
  AGGGCCCCCCACACCTGCTGCGGCCCACAGCATCCCCTACAACAGCTGCAGAGAGAAGGAGCCAAGTTAC
  CCCATGCCTGTCCAGGAGACCCAGGCGCCAGAGTCCCCAGGAGAGAATTCAGAGCAAGCCCTGCAGACGC
  TCAGCCCCAGAGCCATCCCAAGGAATCCAGATGGTGGCCCCCTGGAGTCCTCCTCTGACCTGGCAGCCCT
  CAGCCCTCTGACCTCCAGCGGGCATCAGGAGAAGGACACAGAACTGGGCAGTACCCACACAGCAGGTGCG
  ACCTCCAGCCTCACACCATCCCGTGGGCCTGTGTCTCCATCTGTCTCCTTCCAGCCCCTGGCCCGTTCCA
  CCCCCAGGGCAAGCCGCTTGCCTGGACCCACAGGGTCAGTTGTATCTACTGGCACCTCCTTCTCCTCCTC
  ATCCCCTGGCTTGGCCTCTGCAGGGGCTGCAGAGGGTAAACAGGGTGCAGAGAGTGACCAGGCCGAGCCT
  ATCATCTGCTCCAGTGGGGCAGAGGCACCTGCCAACTCTCTGCCCTCCAAAGTGCCTACCACCTTGATGC
  CTGTGAACACAGTGGCCCTGAAAGTGCCTGCCAACCCAGCATCTGTCAGCACAGTGCCCTCCAAGTTGCC
  AACTAGCTCAAAGCCCCCTGGTGCAGTGCCTTCTAATGCGCTCACCAATCCAGCACCATCCAAATTGCCC
  ATCAACTCAACCCGTGCTGGCATGGTGCCATCCAAAGTGCCTACTAGCATGGTGCTCACCAAGGTGTCTG
  CCAGCACAGTCCCCACTGACGGGAGCAGCAGAAATGAGGAGACCCCAGCAGCTCCAACACCCGCCGGCGC
  CACTGGAGGCAGCTCAGCCTGGCTAGACAGCAGCTTTGAGAATAGGGGCCTTGGGTCGGAGCTGAGTAAG
  CCTGGCGTGCTGGCATCCCAGGTAGACAGCCCGTTCTCGGGCTGCTTCGAGGATCTTGCCATCAGTGCCA
  GCACCTCCTTGGGCATGGGGCCCTGCCATGGCCCAGAGGAGAATGAGTATAAGTCCGAGGGCACCTTTGG
  GATCCACGTGGCTGAGAACCCCAGCATCCAGCTCCTGGAGGGCAACCCTGGGCCACCTGCGGACCCGGAT
  GGCGGCCCCAGGCCACAAGCCGACCGGAAGTTCCAGGAGAGGGAGGTGCCATGCCACAGGCCCTCACCTG
  GGGCTCTGTGGCTCCAGGTGGCTGTGACAGGGGTGCTGGTAGTCACACTCCTGGTGGTGCTGTACCGGCG
  GCGTCTGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208175 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVE
  YFIAALRGCELVDLADEVASVYESYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSY
  PMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEKDTELGSTHTAGA
  TSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEP
  IICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLP
  INSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSFENRGLGSELSK
  PGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPD
  GGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020746
• ORF Size: 1620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020746.5
• RefSeq Size: 11771 bp
• RefSeq ORF: 1623 bp
• Locus ID: 57506
• UniProt ID: Q7Z434
• Cytogenetics: 20p13
• Protein Families: Transmembrane
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway
• MW: 56.6 kDa
• Gene Summary: This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral innate immunity. [provided by RefSeq, Jul 2020]