MYBPH (NM_004997) Human Tagged ORF Clone

CAT#: RC208162

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MYBPH (Myc-DDK-tagged)-Human myosin binding protein H (MYBPH)



  "NM_004997" in other vectors (4)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MYBPH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208162 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAAAAAAACACCTCCGAGGGCCCTGCCTGCAGTCCAGAGGAGACCGCATCTGAATCTGCCAAGG
TGCCCACAGCAGAGCCTCCCGGAGAAGTGGCAGTATCAGAGTCCACCAGAGAAGAGCAGGTGCCCAAGCC
GCAGGCCCCTGCCCCACAGGCCCCTACAGCCTCCACAGCCACTAAGCCTGCACCCCCAAGTGAAGATGTC
CCCAGTGCCCCACTGCTGCTGACCCTGGATGATGTGAGCAGCAGCTCTGTGACTGTGAGCTGGGAGCCCC
CAGAGAGGCTGGGGAGGCTGGGCCTCCAGGGCTATGTGCTGGAGCTCTGCAGAGAGGGAGCCTCGGAGTG
GGTGCCTGTGAGTGCCCGGCCCATGATGGTGACCCAGCAGACTGTGCGGAACCTGGCTCTGGGAGACAAG
TTCCTCCTGCGCGTGTCTGCAGTGAGTTCTGCAGGGGCTGGCCCGCCGGCCATGCTGGACCAGCCCATCC
ACATCCGAGAGAACATTGAGGCCCCCAAGATCCGTGTCCCCCGCCACCTCCGTCAGACCTACATCCGCCA
GGTGGGAGAGACGGTCAACCTGCAAATCCCCTTCCAGGGGAAGCCTAAGCCTCAGGCCACATGGACCCAC
AATGGCCATGCCCTGGACAGCCAGCGGGTGAGCATGCGCACCGGGGACCAGGACTCCATCCTCTTCATTC
GCTCGGCCCAGCGCTCCGACTCTGGCCGCTACGAGCTCACTGTGCGCGTGGAAGACCTGGAGGCCAAGGC
AGTCATTGACATCCTGGTGATTGAGAAACCTGGACCCCCCAGCAGCATCAGGCTCCTGGACGTCTGGGGC
TGCAATGCTGCTCTTCAGTGGACGCCACCCCAGGACACAGGCAACACAGAGCTCCTGGGCTACATGGTGC
AGAAGGCAGACAAAAAGACAGGGCAATGGTTCACAGTGCTGGAGCGCTACCACCCAACCACCTGCACCAT
CTCTGACCTCATCATCGGCAACTCGTACTCCTTCCGGGTCTTCTCAGAAAACCTGTGTGGACTCAGCACC
TCGGCCACCGTCACCAAGGAGCTCGCCCACATCCAGAAGGCAGATATTGCTGCCAAACCTAAAGGGTTTA
TTGAGCGAGACTTCTCAGAAGCCCCCTCATTCACCCAGCCCCTGGCTGACCACACCTCCACCCCTGGCTA
CAGCACCCAGTTGTTCTGCAGTGTCCGAGCTTCACCCAAGCCCAAGATCATCTGGATGAAAAACAAGATG
GAGATCCAGGGCAACCCCAAATACCGCGCCCTCTCTGAGCAAGGCGTCTGCACCCTAGAGATCCGGAAAC
CCAGCCCCTTTGATTCTGGGGTCTACACATGCAAGGCCATAAATGTGCTGGGGGAGGCATCTGTGGACTG
CCGGCTGGAGGTCAAAGCCTCAGCCGCACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208162 protein sequence
Red=Cloning site Green=Tags(s)

MMEKNTSEGPACSPEETASESAKVPTAEPPGEVAVSESTREEQVPKPQAPAPQAPTASTATKPAPPSEDV
PSAPLLLTLDDVSSSSVTVSWEPPERLGRLGLQGYVLELCREGASEWVPVSARPMMVTQQTVRNLALGDK
FLLRVSAVSSAGAGPPAMLDQPIHIRENIEAPKIRVPRHLRQTYIRQVGETVNLQIPFQGKPKPQATWTH
NGHALDSQRVSMRTGDQDSILFIRSAQRSDSGRYELTVRVEDLEAKAVIDILVIEKPGPPSSIRLLDVWG
CNAALQWTPPQDTGNTELLGYMVQKADKKTGQWFTVLERYHPTTCTISDLIIGNSYSFRVFSENLCGLST
SATVTKELAHIQKADIAAKPKGFIERDFSEAPSFTQPLADHTSTPGYSTQLFCSVRASPKPKIIWMKNKM
EIQGNPKYRALSEQGVCTLEIRKPSPFDSGVYTCKAINVLGEASVDCRLEVKASAAH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004997
ORF Size 1431 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004997.3
RefSeq Size 1806 bp
RefSeq ORF 1434 bp
Locus ID 4608
UniProt ID Q13203
Cytogenetics 1q32.1
Domains ig, IGc2, IG, FN3
MW 52.1 kDa
Gene Summary Binds to myosin; probably involved in interaction with thick myofilaments in the A-band.[UniProtKB/Swiss-Prot Function]

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