TAF7L (NM_024885) Human Tagged ORF Clone

CAT#: RC208161

TAF7L (Myc-DDK-tagged)-Human TAF7-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50kDa (TAF7L), transcript variant 1

Product Images

Circular map for RC208161

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF7L
• Synonyms: CT40; TAF2Q
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208161 representing NM_024885
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTGCCCCGAGGGACAGCTCCCCATTTCTTCAGAAAATGATTCAACACCAACAGTTTCAACATCGG
  AAGTAACTAGCCAACAAGAACCCCAAATTCTTGTCGACCGTGGGTCTGAAACCACCTATGAAAGCAGCGC
  CGACATTGCTGGCGACGAAGGCACCCAGATTCCTGCCGACGAGGACACTCAAACGGATGCGGACAGCAGC
  GCCCAGGCTGCTGCCCAGGCCCCAGAAAACTTCCAGGAAGGGAAAGACATGAGTGAAAGCCAGGATGAAG
  TTCCTGATGAAGTTGAGAACCAGTTTATATTGCGTCTGCCTCTGGAACATGCTTGTACTGTCAGGAACCT
  AGCACGTTCTCAAAGTGTCAAGATGAAGGATAAACTAAAAATTGACTTATTGCCTGATGGGCGCCATGCA
  GTTGTTGAAGTAGAAGATGTCCCACTAGCTGCTAAGCTGGTTGACTTGCCTTGTGTTATTGAAAGCCTGA
  GAACGCTTGATAAAAAAACCTTTTATAAAACAGCAGACATTTCTCAGATGCTTGTGTGCACTGCTGATGG
  TGATATCCACCTTTCTCCAGAAGAACCAGCTGCCTCTACTGATCCTAATATAGTCAGGAAAAAAGAAAGG
  GGGAGAGAAGAAAAATGTGTCTGGAAGCATGGCATTACGCCACCACTTAAGAATGTCAGAAAGAAAAGGT
  TCCGGAAAACACAAAAAAAGGTCCCTGATGTCAAAGAAATGGAAAAAAGCAGCTTTACTGAGTACATTGA
  ATCTCCAGACGTGGAAAATGAAGTAAAGAGACTGCTGCGTTCGGATGCTGAAGCCGTAAGTACCCGTTGG
  GAAGTCATTGCTGAAGATGGAACCAAGGAAATAGAAAGTCAAGGCTCCATCCCAGGATTTTTGATATCCT
  CGGGAATGAGCAGCCACAAGCAGGGTCATACCTCGTCAGAATATGATATGCTTCGGGAGATGTTCAGTGA
  TTCTAGAAGTAACAATGATGATGATGAGGATGAGGATGATGAAGATGAGGATGAGGATGAGGATGAGGAT
  GAAGATGAAGACAAAGAAGAGGAGGAGGAAGATTGTTCTGAAGAGTATCTGGAAAGGCAGCTGCAGGCCG
  AGTTTATTGAATCTGGCCAGTATAGGGCAAATGAAGGTACCAGTTCAATAGTCATGGAAATTCAGAAGCA
  GATTGAGAAAAAGGAGAAAAAGCTCCATAAGATTCAGAATAAAGCACAAAGACAGAAGGATCTCATCATG
  AAAGTGGAAAACCTGACACTCAAGAATCATTTTCAGTCTGTGCTGGAGCAGCTTGAGTTACAGGAAAAAC
  AAAAAAATGAGAAGCTCATTTCCCTACAGGAACAGTTGCAGCGTTTTCTGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208161 representing NM_024885
  Red=Cloning site Green=Tags(s)
  
  MECPEGQLPISSENDSTPTVSTSEVTSQQEPQILVDRGSETTYESSADIAGDEGTQIPADEDTQTDADSS
  AQAAAQAPENFQEGKDMSESQDEVPDEVENQFILRLPLEHACTVRNLARSQSVKMKDKLKIDLLPDGRHA
  VVEVEDVPLAAKLVDLPCVIESLRTLDKKTFYKTADISQMLVCTADGDIHLSPEEPAASTDPNIVRKKER
  GREEKCVWKHGITPPLKNVRKKRFRKTQKKVPDVKEMEKSSFTEYIESPDVENEVKRLLRSDAEAVSTRW
  EVIAEDGTKEIESQGSIPGFLISSGMSSHKQGHTSSEYDMLREMFSDSRSNNDDDEDEDDEDEDEDEDED
  EDEDKEEEEEDCSEEYLERQLQAEFIESGQYRANEGTSSIVMEIQKQIEKKEKKLHKIQNKAQRQKDLIM
  KVENLTLKNHFQSVLEQLELQEKQKNEKLISLQEQLQRFLKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024885
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024885.4
• RefSeq Size: 2371 bp
• RefSeq ORF: 1389 bp
• Locus ID: 54457
• UniProt ID: Q5H9L4
• Cytogenetics: Xq22.1
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 52.6 kDa
• Gene Summary: This gene is similar to a mouse gene that encodes a TATA box binding protein-associated factor, and shows testis-specific expression. The encoded protein could be a spermatogenesis-specific component of the DNA-binding general transcription factor complex TFIID. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]