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CDH12 (NM_004061) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC208156L1
Lenti ORF clone of Human cadherin 12, type 2 (N-cadherin 2) (CDH12), Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,390.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDH12
Synonyms CDHB
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC208156).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004061
ORF Size 2382 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004061.2
RefSeq Size 4164 bp
RefSeq ORF 2385 bp
Locus ID 1010
UniProt ID P55289
Cytogenetics 5p14.3
Domains CA, Cadherin_C_term
Protein Families Transmembrane
MW 88.2 kDa
Summary This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_004061" in other vectors (6)
SKU Description Size Price
RC208156 CDH12 (Myc-DDK-tagged)-Human cadherin 12, type 2 (N-cadherin 2) (CDH12) 10 ug
$1,090.00
RC208156L2 Lenti ORF clone of Human cadherin 12, type 2 (N-cadherin 2) (CDH12), mGFP tagged 10 ug
$1,390.00
RC208156L3 Lenti ORF clone of Human cadherin 12, type 2 (N-cadherin 2) (CDH12), Myc-DDK-tagged 10 ug
$1,390.00
RC208156L4 Lenti ORF clone of Human cadherin 12, type 2 (N-cadherin 2) (CDH12), mGFP tagged 10 ug
$1,390.00
RG208156 CDH12 (tGFP-tagged) - Human cadherin 12, type 2 (N-cadherin 2) (CDH12) 10 ug
$1,290.00
SC117606 CDH12 (untagged)-Human cadherin 12, type 2 (N-cadherin 2) (CDH12) 10 ug
$1,092.00

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