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GAS2L3 (NM_174942) Human Tagged ORF Clone

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SKU
RC208141
GAS2L3 (Myc-DDK-tagged)-Human growth arrest-specific 2 like 3 (GAS2L3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $636.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GAS2L3
Synonyms G2L3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208141 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCTGCAATTCAAGTATGGTTTGGAGAAGATCTGCCTCTAAGTCCTCGGAGTCCTCTGACTCCCA
GACACGGACCAGGATTGGCTAATGTTTGTCAGTACGATGAGTGGATAGCTGTGAGGCATGAAGCCACTTT
GTTGCCCATGCAAGAAGATCTGTCAATCTGGTTATCTGGTTTATTAGGTATTAAAGTTAAGGCAGAAAAA
TTATTGGAAGAACTTGATAATGGAGTACTATTATGTCAACTGATTGATGTTCTTCAAAACATGGTGAAAA
CATGCAACTCTGAAGAATCAGGGAATTTTCCAATGAGAAAAGTGCCCTGTAAGAAAGATGCTGCATCAGG
TTCATTCTTTGCTCGGGACAATACCGCAAACTTCCTTCACTGGTGTAGGGACATTGGGGTTGATGAAACT
TACCTCTTTGAATCTGAAGGTTTAGTTTTGCACAAAGATCCAAGACAGGTGTATCTTTGTCTTCTTGAAA
TTGGTCGAATTGTGTCAAGATACGGGGTTGAGCCACCAGTGTTAGTAAAACTTGAGAAAGAAATTGAGTT
AGAAGAGACTTTGCTTAATACTTCTGGGCCTGAAGATTCCATCAGCATTCCAAAATCATGCTGTCGGCAT
GAAGAGCTACATGAAGCTGTTAAACATATTGCTGAGGACCCTCCTTGTAGTTGTTCTCATCGATTTTCTA
TTGAGTATTTATCTGAAGGACGGTACCGACTAGGGGATAAAATACTCTTTATAAGAATGCTTCATGGAAA
ACATGTCATGGTTCGCGTTGGTGGAGGCTGGGATACTCTTCAAGGATTTTTGCTTAAATATGACCCCTGT
CGAATATTACAGTTTGCCACACTAGAACAAAAAATTTTAGCATTTCAAAAAGGAGTTTCTAATGAAAGTG
TACCTGATTCGCCTGCCAGAACACCTCAGCCTCCTGAAATGAATCCTTTGTCAGCAGTTAACATGTTTCA
GAAACAAAATTCAAAACCCAGCGTGCCAGTTAGTATTCCAAAAAGCAAAGAAAAACAGGGACGTCCACCA
GGTGCATTGGTGCCAGCATCTTCACTGAAAGGAGGTAATCTGGGCTCTATGTCAGTCCGTTCTAAATTGC
CAAATTCTCCAGCAGCATCTTCTCATCCCAAGCTCAAGTCTTCAAAAGGCATAACGAAGAAACCGCAGGC
TCCTTCAAACAATGCATCATCTTCACTTGCTTCATTAAATCCAGTAGGTAAAAACACTTCTTCACCAGCT
TTACCAAGAACTGCACCTTGTATATCTGAGTCACCGAGAAAATGTATTTCATCCCCCAATACCCCCAAGG
CCAAGGTTATTCCAGCCCAGAATTCAGCAGATCTGCCCGAGTCCACACTTTTGCCAAATAAGTGTTCAGG
AAAAACTCAACCTAAGTATTTGAAACATAATCATATTTCTTCCAGAGATAATGCAGTATCTCACTTAGCT
GCACATTCAAATTCATCCTCAAAATGTCCCAAGCTGCCTAAAGCAAATATACCTGTAAGACCTAAACCTT
CTTTCCAGTCCTCTGCAAAAATGACAAAAACCAGTTCCAAAACCATAGCCACGGGTCTAGGAACACAGTC
TCAACCATCCGATGGAGCCCCACAAGCAAAGCCAGTCCCAGCACAGAAACTTAAATCGGCCTTGAATTTA
AATCAGCCAGTTTCTGTGTCCTCAGTTTCTCCTGTAAAAGCCACACAGAAATCAAAAGATAAGAATATAG
TTTCAGCTACCAAAAAGCAGCCTCAGAATAAAAGTGCATTTCAGAAGACAGGACCCAGCTCCTTGAAGTC
TCCTGGCCGTACCCCACTGTCCATCGTGAGCCTACCCCAGTCTTCTACCAAAACACAAACTGCACCGAAG
TCAGCACAGACTGTCGCTAAGAGCCAGCATTCAACTAAAGGGCCTCCCAGAAGTGGCAAAACCCCAGCTT
CAATCAGGAAACCACCCTCATCTGTTAAGGATGCAGATAGTGGAGATAAAAAACCTACTGCAAAGAAAAA
GGAAGATGATGACCATTATTTTGTCATGACTGGAAGTAAGAAACCTAGAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208141 protein sequence
Red=Cloning site Green=Tags(s)

MQPAIQVWFGEDLPLSPRSPLTPRHGPGLANVCQYDEWIAVRHEATLLPMQEDLSIWLSGLLGIKVKAEK
LLEELDNGVLLCQLIDVLQNMVKTCNSEESGNFPMRKVPCKKDAASGSFFARDNTANFLHWCRDIGVDET
YLFESEGLVLHKDPRQVYLCLLEIGRIVSRYGVEPPVLVKLEKEIELEETLLNTSGPEDSISIPKSCCRH
EELHEAVKHIAEDPPCSCSHRFSIEYLSEGRYRLGDKILFIRMLHGKHVMVRVGGGWDTLQGFLLKYDPC
RILQFATLEQKILAFQKGVSNESVPDSPARTPQPPEMNPLSAVNMFQKQNSKPSVPVSIPKSKEKQGRPP
GALVPASSLKGGNLGSMSVRSKLPNSPAASSHPKLKSSKGITKKPQAPSNNASSSLASLNPVGKNTSSPA
LPRTAPCISESPRKCISSPNTPKAKVIPAQNSADLPESTLLPNKCSGKTQPKYLKHNHISSRDNAVSHLA
AHSNSSSKCPKLPKANIPVRPKPSFQSSAKMTKTSSKTIATGLGTQSQPSDGAPQAKPVPAQKLKSALNL
NQPVSVSSVSPVKATQKSKDKNIVSATKKQPQNKSAFQKTGPSSLKSPGRTPLSIVSLPQSSTKTQTAPK
SAQTVAKSQHSTKGPPRSGKTPASIRKPPSSVKDADSGDKKPTAKKKEDDDHYFVMTGSKKPRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_174942
ORF Size 2082 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174942.1, NP_777602.1
RefSeq Size 2341 bp
RefSeq ORF 2085 bp
Locus ID 283431
UniProt ID Q86XJ1
Cytogenetics 12q23.1
MW 75.2 kDa
Summary Cytoskeletal linker protein. May promote and stabilize the formation of the actin and microtubule network.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_174942" in other vectors (4)
SKU Description Size Price
RC208141L3 Lenti ORF clone of Human growth arrest-specific 2 like 3 (GAS2L3), Myc-DDK-tagged 10 ug
$936.00
RC208141L4 Lenti ORF clone of Human growth arrest-specific 2 like 3 (GAS2L3), mGFP tagged 10 ug
$936.00
RG208141 GAS2L3 (tGFP-tagged) - Human growth arrest-specific 2 like 3 (GAS2L3) 10 ug
$836.00
SC125667 GAS2L3 (untagged)-Human growth arrest-specific 2 like 3 (GAS2L3) 10 ug
$636.00

file-alt Citations

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