CRKL (NM_005207) Human Tagged ORF Clone
CAT#: RC208129
CRKL (Myc-DDK-tagged)-Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL)
Specifications
Product Data | |
Product Name | CRKL (NM_005207) Human Tagged ORF Clone |
Symbol | CRKL |
Synonyms | v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian)-like |
Vector | pCMV6-Entry |
Sequence Data |
>RC208129 representing NM_005207
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCCGCCAGGTTCGACTCCTCGGACCGCTCCGCCTGGTATATGGGGCCGGTGTCTCGCCAGGAGG CGCAGACCCGGCTCCAGGGCCAGCGCCACGGTATGTTCCTCGTCCGCGATTCTTCCACCTGCCCTGGGGA CTATGTGCTGTCGGTGTCCGAGAACTCGCGGGTCTCCCACTACATCATCAACTCGCTGCCCAACCGCCGT TTTAAGATCGGGGACCAGGAATTTGACCATTTGCCGGCCCTGCTGGAGTTTTACAAGATCCACTACCTGG ACACCACCACCCTCATCGAGCCTGCGCCCAGGTATCCAAGCCCACCAATGGGATCTGTCTCAGCACCCAA CCTGCCTACAGCAGAAGATAACCTGGAATATGTACGGACTCTGTATGATTTTCCTGGGAATGATGCCGAA GACCTGCCCTTTAAAAAGGGTGAGATCCTAGTGATAATAGAGAAGCCTGAAGAACAGTGGTGGAGTGCCC GGAACAAGGATGGCCGGGTTGGGATGATTCCTGTCCCTTATGTCGAAAAGCTTGTGAGATCCTCACCACA CGGAAAGCATGGAAATAGGAATTCCAACAGTTATGGGATCCCAGAACCTGCTCATGCATACGCTCAACCT CAGACCACAACTCCTCTACCTGCAGTTTCCGGTTCTCCTGGGGCAGCAATCACCCCTTTGCCATCCACAC AGAATGGACCTGTCTTTGCGAAAGCAATCCAGAAAAGAGTACCCTGTGCTTATGACAAGACTGCCTTGGC ATTAGAGGTTGGTGACATCGTGAAAGTCACAAGGATGAATATAAATGGCCAGTGGGAAGGCGAAGTGAAC GGGCGCAAAGGGCTTTTCCCCTTTACGCACGTCAAAATCTTTGACCCTCAAAACCCAGATGAAAACGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208129 representing NM_005207
Red=Cloning site Green=Tags(s) MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRR FKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAE DLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQP QTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVN GRKGLFPFTHVKIFDPQNPDENE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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Tag | Myc-DDK |
ACCN | NM_005207 |
ORF Size | 909 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_005207.1, NM_005207.2, NM_005207.3, NP_005198 |
RefSeq Size | 5235 |
RefSeq ORF | 912 |
Locus ID | 1399 |
Cytogenetics | 22q11.21 |
Domains | SH2, SH3 |
Protein Families | Druggable Genome |
Protein Pathways | MAPK signaling pathway, ErbB signaling pathway, Chemokine signaling pathway, Focal adhesion, Fc gamma R-mediated phagocytosis, Neurotrophin signaling pathway, Regulation of actin cytoskeleton, Insulin signaling pathway, Pathways in cancer, Renal cell carcinoma, Chronic myeloid leukemia |
MW | 33.6 kDa |
Gene Summary | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]. |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
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MicroRNA-320 inhibits invasion and induces apoptosis by targeting CRKL and inhibiting ERK and AKT signaling in gastric cancer cells
,Zhao, Y;Dong, Q;Wang, E;,
Onco Targets Ther 2017
,PubMed ID 28255248
[CRKL]
|
CRKL overexpression promotes cell proliferation and inhibits apoptosis in endometrial carcinoma
,CAI, L;WANG, H;YANG, Q;,
ONCOLOGY LETTERS 2016
,PubMed ID 28123521
[CRKL]
|
Crk-like adapter protein is overexpressed in cervical carcinoma, facilitates proliferation, invasion and chemoresistance, and regulates Src and Akt signaling
,JI, H;LI, B;ZHANG, S;HE, Z;ZHOU, Y;OUYANG, L;,
Oncology Letters 2016
[CRKL]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
cDNA Clone Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG208129 | CRKL (GFP-tagged) - Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) |
USD 560.00 |
|
RC208129L1 | Lenti ORF clone of Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) , Myc-DDK-tagged |
USD 700.00 |
|
RC208129L4V | Lenti ORF particles, CRKL (mGFP-tagged) - Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), 200ul, >10^7 TU/mL |
USD 1,020.00 |
|
RC208129L3 | Lenti ORF clone of Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) , Myc-DDK-tagged |
USD 700.00 |
|
SC116881 | CRKL (untagged)-Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) |
USD 300.00 |
|
RC208129L4 | Lenti ORF clone of Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), mGFP tagged |
USD 760.00 |
|
RC208129L2 | Lenti ORF clone of Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), mGFP tagged |
USD 760.00 |
|
RC208129L3V | Lenti ORF particles, CRKL (Myc-DDK tagged) - Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) , 200ul, >10^7 TU/mL |
USD 910.00 |
|
RC208129L1V | Lenti ORF particles, CRKL (Myc-DDK tagged) - Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL) , 200ul, >10^7 TU/mL |
USD 910.00 |
|
RC208129L2V | Lenti ORF particles, CRKL (mGFP-tagged) - Human v-crk sarcoma virus CT10 oncogene homolog (avian)-like (CRKL), 200ul, >10^7 TU/mL |
USD 1,020.00 |
USD 299.00
USD 550.00