HBS1L (NM_006620) Human Tagged ORF Clone

CAT#: RC208125

HBS1L (Myc-DDK-tagged)-Human HBS1-like (S. cerevisiae) (HBS1L), transcript variant 1

Product Images

Circular map for RC208125

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HBS1L (Cat# RC208125, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HBS1L(Cat# TA800554). Positive lysates LY416509 (100ug) and LC416509 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416509) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208125 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HBS1L
• Synonyms: EF-1a; eRF3c; ERFS; HBS1; HSPC276
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208125 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGCATCGGAATGTTCGAGGCTATAACTACGATGAAGATTTTGAAGATGATGATCTCTACGGCC
  AGTCTGTAGAGGATGATTATTGTATTTCGCCGTCAACAGCTGCTCAGTTTATTTATTCACGGCGTGACAA
  ACCTTCCGTTGAGCCTGTGGAAGAATATGATTATGAAGATCTGAAAGAATCTTCCAATTCTGTTTCAAAC
  CATCAGCTCAGTGGATTTGATCAAGCTCGTCTTTATTCATGCCTTGATCACATGAGAGAGGTACTTGGAG
  ATGCTGTGCCAGATGAAATATTAATTGAAGCAGTTCTGAAGAACAAGTTTGATGTGCAGAAGGCTTTGTC
  AGGGGTTCTGGAACAAGATAGAGTGCAGAGTTTGAAGGACAAGAATGAGGCAACAGTATCTACAGGAAAG
  ATAGCAAAAGGAAAACCAGTAGATTCCCAGACATCGCGAAGTGAATCTGAAATTGTGCCAAAAGTTGCTA
  AAATGACTGTATCTGGAAAGAAGCAAACTATGGGATTTGAAGTGCCTGGAGTATCTTCTGAAGAAAATGG
  TCATAGTTTCCACACACCTCAAAAAGGACCGCCCATTGAAGATGCCATTGCTTCTTCCGATGTTCTTGAG
  ACTGCTTCTAAATCTGCTAATCCACCCCACACGATTCAAGCATCAGAAGAGCAGAGTTCAACCCCAGCAC
  CGGTGAAAAAGTCTGGCAAGCTGAGGCAGCAAATAGATGTGAAGGCGGAACTGGAGAAGCGGCAAGGAGG
  GAAGCAGCTACTCAACTTAGTGGTCATTGGTCATGTTGATGCTGGGAAAAGTACTCTGATGGGCCATATG
  CTTTATCTTCTGGGTAATATAAACAAAAGAACTATGCATAAGTATGAACAGGAGTCTAAAAAGGCTGGCA
  AAGCTTCGTTTGCATATGCATGGGTCTTGGATGAAACTGGCGAAGAAAGGGAAAGGGGAGTAACCATGGA
  TGTTGGTATGACAAAGTTTGAAACCACAACCAAAGTTATTACATTAATGGATGCTCCAGGCCATAAGGAC
  TTCATTCCAAATATGATTACAGGAGCAGCCCAGGCGGATGTAGCTGTTTTAGTTGTAGATGCCAGCAGGG
  GAGAGTTTGAAGCTGGATTTGAGACTGGAGGACAAACACGAGAGCATGGACTCTTGGTCCGTTCTCTGGG
  AGTGACGCAGCTTGCAGTTGCAGTTAATAAAATGGATCAGGTTAATTGGCAACAAGAAAGGTTTCAAGAG
  ATTACTGGAAAACTTGGGCACTTTCTTAAGCAAGCAGGTTTTAAGGAGAGTGATGTAGGTTTTATTCCTA
  CAAGTGGTCTCAGTGGTGAAAATCTAATCACAAGATCTCAGTCAAGTGAACTCACAAAATGGTATAAAGG
  ACTATGTTTATTAGAACAAATTGATTCCTTTAAGCCTCCCCAGCGATCTATTGACAAACCTTTTAGATTA
  TGTGTGTCCGATGTTTTCAAAGATCAAGGATCTGGATTTTGCATAACTGGTAAAATAGAAGCTGGTTATA
  TCCAAACTGGTGACCGACTACTGGCAATGCCTCCTAATGAAACTTGTACCGTGAAAGGAATCACTCTGCA
  TGATGAACCTGTCGACTGGGCGGCAGCAGGCGATCATGTTAGTCTTACTTTGGTTGGGATGGATATCATC
  AAAATCAATGTTGGCTGCATATTTTGTGGCCCCAAAGTACCCATTAAAGCTTGCACTCGTTTCAGAGCCC
  GAATCCTCATCTTTAATATTGAAATTCCTATCACTAAAGGATTTCCTGTGCTGTTACACTACCAAACTGT
  CAGTGAACCCGCCGTTATTAAACGATTGATTAGTGTCTTAAACAAAAGCACGGGTGAAGTCACAAAGAAA
  AAGCCTAAGTTTTTGACTAAAGGCCAGAATGCATTGGTAGAGCTACAGACACAAAGACCAATAGCTCTTG
  AGCTATATAAAGACTTTAAAGAGCTGGGGAGGTTCATGCTACGTTACGGTGGTTCTACAATAGCTGCTGG
  TGTTGTCACTGAGATAAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208125 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYEDLKESSNSVSN
  HQLSGFDQARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVLEQDRVQSLKDKNEATVSTGK
  IAKGKPVDSQTSRSESEIVPKVAKMTVSGKKQTMGFEVPGVSSEENGHSFHTPQKGPPIEDAIASSDVLE
  TASKSANPPHTIQASEEQSSTPAPVKKSGKLRQQIDVKAELEKRQGGKQLLNLVVIGHVDAGKSTLMGHM
  LYLLGNINKRTMHKYEQESKKAGKASFAYAWVLDETGEERERGVTMDVGMTKFETTTKVITLMDAPGHKD
  FIPNMITGAAQADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERFQE
  ITGKLGHFLKQAGFKESDVGFIPTSGLSGENLITRSQSSELTKWYKGLCLLEQIDSFKPPQRSIDKPFRL
  CVSDVFKDQGSGFCITGKIEAGYIQTGDRLLAMPPNETCTVKGITLHDEPVDWAAAGDHVSLTLVGMDII
  KINVGCIFCGPKVPIKACTRFRARILIFNIEIPITKGFPVLLHYQTVSEPAVIKRLISVLNKSTGEVTKK
  KPKFLTKGQNALVELQTQRPIALELYKDFKELGRFMLRYGGSTIAAGVVTEIKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006620
• ORF Size: 2052 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006620.4
• RefSeq Size: 7163 bp
• RefSeq ORF: 2055 bp
• Locus ID: 10767
• UniProt ID: Q9Y450
• Cytogenetics: 6q23.3
• Domains: GTP_EFTU, GTP_EFTU_D3, GTP_EFTU_D2
• MW: 75.5 kDa
• Gene Summary: This gene encodes a member of the GTP-binding elongation factor family. It is expressed in multiple tissues with the highest expression in heart and skeletal muscle. The intergenic region of this gene and the MYB gene has been identified to be a quantitative trait locus (QTL) controlling fetal hemoglobin level, and this region influnces erythrocyte, platelet, and monocyte counts as well as erythrocyte volume and hemoglobin content. DNA polymorphisms at this region associate with fetal hemoglobin levels and pain crises in sickle cell disease. A single nucleotide polymorphism in exon 1 of this gene is significantly associated with severity in beta-thalassemia/Hemoglobin E. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, May 2009]