WIPI1 (NM_017983) Human Tagged Lenti ORF Clone
- LentiORF®
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | WIPI1 |
| Synonyms | ATG18; ATG18A; WIPI49 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_017983 |
| ORF Size | 1338 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_017983.3 |
| RefSeq Size | 1939 bp |
| RefSeq ORF | 1341 bp |
| Locus ID | 55062 |
| UniProt ID | Q5MNZ9 |
| Cytogenetics | 17q24.2 |
| Domains | WD40 |
| MW | 48.7 kDa |
| Summary | This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_017983" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208094 | WIPI1 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 1 (WIPI1) | 10 ug |
$457.00
|
|
| RC208094L1 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 1 (WIPI1), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC208094L2 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 1 (WIPI1), mGFP tagged | 10 ug |
$757.00
|
|
| RC208094L3 | Lenti ORF clone of Human WD repeat domain, phosphoinositide interacting 1 (WIPI1), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RG208094 | WIPI1 (tGFP-tagged) - Human WD repeat domain, phosphoinositide interacting 1 (WIPI1) | 10 ug |
$489.00
$657.00
|
|
| SC310381 | WIPI1 (untagged)-Human WD repeat domain, phosphoinositide interacting 1 (WIPI1) | 10 ug |
$457.00
|
|
| SC322622 | WIPI1 (untagged)-Human WD repeat domain, phosphoinositide interacting 1 (WIPI1) | 10 ug |
$457.00
|
|
Plasmid Map for RC208094L4
Download sequence for
Cloning scheme for RC208094L4
Circular map for RC208094L4
Citations
- Price:
- Actual Price: