GRF2 (RAPGEF1) (NM_198679) Human Tagged Lenti ORF Clone
- LentiORF®
SKU
RC208092L4
Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2, mGFP tagged
$1,253.00
3 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GRF2 |
| Synonyms | C3G; GRF2 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_198679 |
| ORF Size | 3285 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_198679.1 |
| RefSeq Size | 6204 bp |
| RefSeq ORF | 3288 bp |
| Locus ID | 2889 |
| UniProt ID | Q13905 |
| Cytogenetics | 9q34.13 |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma |
| MW | 122.6 kDa |
| Summary | This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_198679" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208092 | RAPGEF1 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2 | 10 ug |
$953.00
|
|
| RC208092L3 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,253.00
|
|
| RG208092 | RAPGEF1 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2 | 10 ug |
$789.00
$1,153.00
|
|
| SC307763 | RAPGEF1 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2 | 10 ug |
$954.00
|
|
Plasmid Map for RC208092L4
Download sequence for
Cloning scheme for RC208092L4
Circular map for RC208092L4
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
- Price:
- Actual Price: