ARHGEF6 (NM_004840) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC208074L1
Lenti-ORF clone of ARHGEF6 (Myc-DDK-tagged)-Human Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (ARHGEF6)
  $1,011.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARHGEF6
Synonyms alpha-PIX; alphaPIX; Cool-2; COOL2; MRX46; PIXA
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC208074).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004840
ORF Size 2328 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004840.2
RefSeq Size 5282 bp
RefSeq ORF 2331 bp
Locus ID 9459
UniProt ID Q15052
Cytogenetics Xq26.3
Domains CH, PH, RhoGEF, SH3
Protein Families Druggable Genome
Protein Pathways Pancreatic cancer, Regulation of actin cytoskeleton
MW 87.3 kDa
Summary Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific cognitive disability. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_004840" in other vectors (6)
SKU Description Size Price
RC208074 ARHGEF6 (Myc-DDK-tagged)-Human Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (ARHGEF6) 10 ug
$589.00 $711.00
RC208074L2 Lenti-ORF clone of ARHGEF6 (mGFP-tagged)-Human Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (ARHGEF6) 10 ug
$1,011.00
RC208074L3 Lenti-ORF clone of ARHGEF6 (Myc-DDK-tagged)-Human Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (ARHGEF6) 10 ug
$1,011.00
RC208074L4 Lenti-ORF clone of ARHGEF6 (mGFP-tagged)-Human Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (ARHGEF6) 10 ug
$1,011.00
RG208074 ARHGEF6 (tGFP-tagged) - Human Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (ARHGEF6) 10 ug
$911.00
SC100230 ARHGEF6 (untagged)-Human Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (ARHGEF6) 10 ug
$711.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.