NR2C1 (NM_001032287) Human Tagged ORF Clone

CAT#: RC208060

NR2C1 (Myc-DDK-tagged)-Human nuclear receptor subfamily 2, group C, member 1 (NR2C1), transcript variant 2

Product Images

Circular map for RC208060

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NR2C1 (Cat# RC208060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR2C1(Cat# TA803381). Positive lysates LY422297 (100ug) and LC422297 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY422297) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208060 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NR2C1
• Synonyms: TR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208060 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCATAGAAGAAATTGCACATCAAATTATTGAACAACAGATGGGAGAGATTGTTACAGAGCAGC
  AAACTGGGCAGAAAATCCAGATTGTGACAGCACTTGATCATAATACCCAAGGCAAGCAGTTCATTCTGAC
  AAATCACGACGGCTCTACTCCAAGCAAAGTCATTCTGGCCAGGCAAGATTCCACTCCGGGAAAAGTTTTC
  CTTACAACTCCAGATGCAGCAGGTGTCAACCAGTTATTTTTTACCACTCCTGATCTGTCTGCACAACACC
  TGCAGCTCCTAACAGATAATTCTCCAGACCAAGGACCAAATAAGGTTTTTGATCTTTGCGTAGTATGTGG
  AGACAAAGCATCAGGACGTCATTATGGAGCAGTAACTTGTGAAGGCTGCAAAGGATTTTTTAAAAGAAGC
  ATCCGAAAAAATTTAGTATATTCATGTCGAGGATCAAAGGATTGTATTATTAATAAGCACCACCGAAACC
  GCTGTCAATACTGCAGGTTACAGAGATGTATTGCGTTTGGAATGAAGCAAGACTCTGTCCAATGTGAAAG
  AAAACCCATTGAAGTATCACGAGAAAAATCTTCCAACTGTGCCGCTTCAACAGAAAAAATCTATATCCGA
  AAGGACCTTCGTAGCCCATTAACTGCAACTCCAACTTTTGTAACAGATAGTGAAAGTACAAGGTCAACAG
  GACTGTTAGATTCAGGAATGTTCATGAATATTCATCCATCTGGAGTAAAAACTGAGTCAGCTGTGCTGAT
  GACATCAGATAAGGCTGAATCATGTCAGGGAGATTTAAGTACATTGGCCAATGTGGTTACATCATTAGCG
  AATCTTGGAAAAACTAAAGATCTTTCTCAAAATAGTAATGAAATGTCTATGATTGAAAGCTTAAGCAATG
  ATGATACCTCTTTGTGTGAATTTCAAGAAATGCAGACCAACGGTGATGTTTCAAGGGCATTTGACACTCT
  TGCAAAAGCATTGAATCCTGGAGAGAGCACAGCCTGCCAGAGCTCAGTAGCGGGCATGGAAGGAAGTGTA
  CACCTAATCACTGGAGATTCAAGCATAAATTACACCGAAAAAGAGGGGCCACTTCTCAGCGATTCACATG
  TAGCTTTCAGGCTCACCATGCCTTCTCCTATGCCTGAGTACCTGAATGTGCACTACATTGGGGAGTCTGC
  CTCCAGACTGCTGTTCTTATCAATGCACTGGGCACTTTCGATTCCTTCTTTCCAGGCTCTAGGGCAAGAA
  AACAGCATATCACTGGTGAAAGCTTACTGGAATGAACTTTTTACTCTTGGTCTTGCCCAGTGCTGGCAAG
  TGATGAATGTAGCAACTATATTAGCAACATTTGTCAATTGTCTTCACAATAGTCTTCAACAAGCAGAGGG
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208060 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATIEEIAHQIIEQQMGEIVTEQQTGQKIQIVTALDHNTQGKQFILTNHDGSTPSKVILARQDSTPGKVF
  LTTPDAAGVNQLFFTTPDLSAQHLQLLTDNSPDQGPNKVFDLCVVCGDKASGRHYGAVTCEGCKGFFKRS
  IRKNLVYSCRGSKDCIINKHHRNRCQYCRLQRCIAFGMKQDSVQCERKPIEVSREKSSNCAASTEKIYIR
  KDLRSPLTATPTFVTDSESTRSTGLLDSGMFMNIHPSGVKTESAVLMTSDKAESCQGDLSTLANVVTSLA
  NLGKTKDLSQNSNEMSMIESLSNDDTSLCEFQEMQTNGDVSRAFDTLAKALNPGESTACQSSVAGMEGSV
  HLITGDSSINYTEKEGPLLSDSHVAFRLTMPSPMPEYLNVHYIGESASRLLFLSMHWALSIPSFQALGQE
  NSISLVKAYWNELFTLGLAQCWQVMNVATILATFVNCLHNSLQQAEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032287
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032287.3
• RefSeq Size: 1903 bp
• RefSeq ORF: 1404 bp
• Locus ID: 7181
• UniProt ID: P13056
• Cytogenetics: 12q22
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• MW: 51.2 kDa
• Gene Summary: This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]