OriGene Logo

CWC15 (NM_016403) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC208046
CWC15 (Myc-DDK-tagged)-Human CWC15 spliceosome-associated protein homolog (S. cerevisiae) (CWC15)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Size
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA360670 CWC15 Antibody - middle region 100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags 30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA) 5 x 200 ul
$160.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each 100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug 10 ug
$650.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL 5 ml
$720.00

Other products for "CWC15 or NM_016403"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CWC15
Synonyms AD002; C11orf5; Cwf15; HSPC148; ORF5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208046 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAACAGCAGCCAGGCCAACCTTTGAACCTGCCAGAGGTGGAAGGGGAAAAGGAGAAGGTGATTTGA
GCCAACTTTCAAAGCAGTATTCAAGCAGAGACCTACCCTCTCATACAAAGATAAAATACAGACAGACTAC
TCAGGATGCCCCTGAAGAGGTTCGTAACCGTGACTTCAGGAGAGAGTTGGAAGAAAGAGAGAGAGCTGCT
GCAAGAGAGAAAAATAGGGATCGTCCAACCCGAGAACATACAACCTCCTCTTCAGTGTCAAAAAAGCCAC
GGTTAGACCAGATTCCTGCCGCCAACCTTGATGCAGATGACCCTCTAACAGATGAGGAAGATGAAGATTT
TGAAGAAGAAAGTGATGATGATGATACTGCAGCTCTTCTTGCAGAACTGGAAAAAATTAAAAAAGAAAGA
GCTGAAGAGCAGGCCAGGAAGGAACAAGAACAAAAAGCTGAAGAAGAGAGGATTCGTATGGAAAACATTC
TGAGCGGAAACCCTCTCCTTAATCTCACTGGCCCATCCCAGCCTCAGGCCAACTTCAAAGTTAAAAGAAG
GTGGGATGATGACGTTGTCTTCAAGAACTGTGCAAAAGGTGTAGATGACCAGAAGAAAGACAAAAGATTT
GTAAATGACACACTGCGATCTGAATTTCACAAAAAGTTCATGGAGAAATATATTAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208046 protein sequence
Red=Cloning site Green=Tags(s)

MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAA
AREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKER
AEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRF
VNDTLRSEFHKKFMEKYIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016403
ORF Size 687 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016403.4
RefSeq Size 1592 bp
RefSeq ORF 690 bp
Locus ID 51503
UniProt ID Q9P013
Cytogenetics 11q21
Domains Cwf_Cwc_15
Protein Pathways Spliceosome
MW 26.6 kDa
Summary Involved in pre-mRNA splicing as component of the spliceosome (PubMed:28502770, PubMed:28076346). Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_016403" in other vectors (4)
SKU Description Size Price
RC208046L3 Lenti ORF clone of Human CWC15 spliceosome-associated protein homolog (S. cerevisiae) (CWC15), Myc-DDK-tagged 10 ug
$600.00
RC208046L4 Lenti ORF clone of Human CWC15 spliceosome-associated protein homolog (S. cerevisiae) (CWC15), mGFP tagged 10 ug
$600.00
RG208046 CWC15 (tGFP-tagged) - Human CWC15 spliceosome-associated protein homolog (S. cerevisiae) (CWC15) 10 ug
$489.00 $500.00
SC108674 CWC15 (untagged)-Human CWC15 spliceosome-associated protein homolog (S. cerevisiae) (CWC15) 10 ug
$300.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.