L3MBTL1 (NM_015478) Human Tagged ORF Clone

CAT#: RC208022

L3MBTL1 (Myc-DDK-tagged)-Human l(3)mbt-like 1 (Drosophila) (L3MBTL1), transcript variant I

Product Images

Circular map for RC208022

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  Western blot validation of overexpression lysate (Cat# LY414530) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208022 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified L3MBTL1 protein (Cat# TP308022). The protein was produced from HEK293T cells transfected with L3MBTL1 cDNA clone (Cat# RC208022) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3MBTL1
• Synonyms: dJ138B7.3; H-L(3)MBT; L3MBTL; ZC2HC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208022 representing NM_015478
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGAAGAGAGGGCCATGGCACCGACTCCGAGATGGGTCAAGGACCCGTACGGGAGTCGCAATCCT
  CAGACCCTCCCGCGCTCCAGTTCCGGATAAGCGAGTATAAGCCGCTGAACATGGCGGGAGTGGAGCAGCC
  CCCGACCCCCGAGCTGCGGCAGGAAGGCGTGACCGAATACGAAGATGGCGGGGCCCCGGCGGGAGATGGC
  GAGGCGGGCCCCCAACAGGCGGAGGACCACCCCCAGAATCCTCCAGAAGATCCCAATCAGGACCCCCCAG
  AGGATGATAGCACCTGTCAGTGCCAGGCGTGCGGGCCTCACCAAGCCGCGGGTCCAGATCTTGGTTCCTC
  TAATGATGGCTGCCCTCAGCTGTTCCAGGAGCGGTCAGTCATAGTGGAGAACTCCTCAGGCTCTACCAGC
  GCTTCTGAGCTCCTCAAACCCATGAAGAAGAGGAAGCGCAGGGAATACCAGAGCCCATCAGAGGAGGAGT
  CGGAGCCAGAGGCCATGGAGAAGCAAGAAGAAGGAAAGGACCCAGAGGGACAACCCACTGCTAGCACCCC
  AGAGAGTGAGGAGTGGAGCAGCAGCCAGCCTGCAACAGGTGAGAAGAAGGAATGCTGGTCGTGGGAGTCC
  TACCTAGAGGAGCAGAAGGCCATTACTGCTCCAGTCAGCCTCTTCCAGGACTCCCAGGCAGTCACTCACA
  ACAAGAATGGCTTCAAACTGGGCATGAAGTTGGAAGGCATTGACCCTCAACACCCGTCCATGTACTTCAT
  CCTCACCGTGGCTGAGGTATGTGGCTATCGCCTACGCCTGCACTTTGATGGGTATTCTGAGTGCCATGAC
  TTCTGGGTCAATGCCAACTCCCCTGACATTCACCCTGCTGGCTGGTTCGAGAAGACGGGCCACAAGCTGC
  AGCCTCCCAAAGGTTACAAGGAGGAGGAGTTCAGCTGGAGCCAGTACCTGCGCAGCACAAGAGCTCAGGC
  TGCCCCCAAGCACCTGTTTGTGAGCCAGAGCCACAGTCCCCCACCCCTGGGCTTCCAGGTGGGCATGAAG
  CTGGAGGCTGTTGACCGCATGAACCCGTCCCTTGTCTGCGTGGCCAGTGTGACCGATGTGGTGGACAGCC
  GCTTCCTGGTGCACTTTGACAACTGGGATGATACTTATGACTACTGGTGTGATCCCAGCAGCCCCTACAT
  CCACCCAGTGGGCTGGTGCCAGAAGCAAGGAAAGCCCCTCACCCCTCCACAAGACTACCCAGACCCTGAT
  AACTTCTGTTGGGAGAAATATCTGGAAGAAACTGGGGCCTCTGCTGTCCCCACCTGGGCCTTCAAGGTGC
  GACCCCCTCACAGCTTCCTGGTCAATATGAAGCTGGAGGCTGTGGACCGCAGGAACCCAGCCCTGATTCG
  CGTGGCCAGCGTGGAGGATGTGGAGGACCATCGGATAAAGATCCACTTTGATGGCTGGAGTCATGGCTAT
  GATTTCTGGATCGACGCTGACCACCCAGACATCCACCCTGCCGGCTGGTGCTCCAAGACAGGACATCCCC
  TGCAGCCTCCTCTCGGACCCAGAGAGCCCAGCTCTGCCTCCCCTGGGGGCTGTCCCCCTCTCAGCTATAG
  GAGCCTGCCCCACACTAGGACCTCCAAATACAGCTTTCACCACCGGAAGTGCCCCACTCCTGGTTGCGAC
  GGCTCTGGCCATGTCACAGGCAAGTTCACAGCTCACCATTGCCTCTCAGGCTGCCCACTGGCTGAGAGGA
  ACCAGAGCCGGCTGAAAGCGGAGCTGTCTGACTCGGAGGCCTCAGCCCGCAAGAAGAACCTCTCAGGCTT
  CTCCCCAAGGAAGAAGCCTCGCCATCACGGCCGAATTGGACGCCCTCCGAAGTATCGAAAGATTCCGCAG
  GAAGATTTCCAGACCCTCACGCCCGATGTCGTGCACCAGTCCCTCTTCATGTCAGCCCTGTCGGCCCACC
  CTGACCGCTCACTCTCAGTGTGCTGGGAGCAGCACTGCAAGCTCCTGCCAGGAGTAGCGGGCATCTCAGC
  CTCGACAGTCGCCAAGTGGACCATCGATGAGGTCTTCGGCTTTGTTCAGACCCTGACAGGTTGTGAGGAC
  CAAGCACGCCTCTTCAAAGACGAGGCAAGAATAGTCAGAGTGACCCATGTATCTGGGAAGACTCTAGTCT
  GGACTGTGGCCCAGCTTGGGGACCTTGTGTGCTCAGATCATCTTCAGGAAGGAAAAGGCATCCTGGAGAC
  AGGAGTCCATTCACTCCTCTGCTCTCTACCCACTCATTTGCTTGCCAAACTTAGCTTTGCCAGTGATAGT
  CAATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208022 representing NM_015478
  Red=Cloning site Green=Tags(s)
  
  MRRREGHGTDSEMGQGPVRESQSSDPPALQFRISEYKPLNMAGVEQPPTPELRQEGVTEYEDGGAPAGDG
  EAGPQQAEDHPQNPPEDPNQDPPEDDSTCQCQACGPHQAAGPDLGSSNDGCPQLFQERSVIVENSSGSTS
  ASELLKPMKKRKRREYQSPSEEESEPEAMEKQEEGKDPEGQPTASTPESEEWSSSQPATGEKKECWSWES
  YLEEQKAITAPVSLFQDSQAVTHNKNGFKLGMKLEGIDPQHPSMYFILTVAEVCGYRLRLHFDGYSECHD
  FWVNANSPDIHPAGWFEKTGHKLQPPKGYKEEEFSWSQYLRSTRAQAAPKHLFVSQSHSPPPLGFQVGMK
  LEAVDRMNPSLVCVASVTDVVDSRFLVHFDNWDDTYDYWCDPSSPYIHPVGWCQKQGKPLTPPQDYPDPD
  NFCWEKYLEETGASAVPTWAFKVRPPHSFLVNMKLEAVDRRNPALIRVASVEDVEDHRIKIHFDGWSHGY
  DFWIDADHPDIHPAGWCSKTGHPLQPPLGPREPSSASPGGCPPLSYRSLPHTRTSKYSFHHRKCPTPGCD
  GSGHVTGKFTAHHCLSGCPLAERNQSRLKAELSDSEASARKKNLSGFSPRKKPRHHGRIGRPPKYRKIPQ
  EDFQTLTPDVVHQSLFMSALSAHPDRSLSVCWEQHCKLLPGVAGISASTVAKWTIDEVFGFVQTLTGCED
  QARLFKDEARIVRVTHVSGKTLVWTVAQLGDLVCSDHLQEGKGILETGVHSLLCSLPTHLLAKLSFASDS
  QY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015478
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015478.7
• RefSeq Size: 3228 bp
• RefSeq ORF: 2319 bp
• Locus ID: 26013
• UniProt ID: Q9Y468
• Cytogenetics: 20q13.12
• Domains: zf-C2HC, MBT
• Protein Families: Transcription Factors
• MW: 85.7 kDa
• Gene Summary: This gene represents a polycomb group gene. The encoded protein functions to regulate gene activity, likely via chromatin modification. The encoded protein may also be necessary for mitosis. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]