NADPH oxidase 4 (NOX4) (NM_016931) Human Tagged ORF Clone

CAT#: RC208007

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NOX4 (Myc-DDK-tagged)-Human NADPH oxidase 4 (NOX4), transcript variant 1



  "NM_016931" in other vectors (7)

Reconstitution Protocol

USD 538.00


Availability*
4 Weeks

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NOX4
Synonyms KOX; KOX-1; RENOX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208007 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTGTCCTGGAGGAGCTGGCTCGCCAACGAAGGGGTTAAACACCTCTGCCTGTTCATCTGGCTCT
CCATGAATGTCCTGCTTTTCTGGAAAACCTTCTTGCTGTATAACCAAGGGCCAGAGTATCACTACCTCCA
CCAGATGTTGGGGCTAGGATTGTGTCTAAGCAGAGCCTCAGCATCTGTTCTTAACCTCAACTGCAGCCTT
ATCCTTTTACCCATGTGCCGAACACTCTTGGCTTACCTCCGAGGATCACAGAAGGTTCCAAGCAGGAGAA
CCAGGAGATTGTTGGATAAAAGCAGAACATTCCATATTACCTGTGGTGTTACTATCTGTATTTTCTCAGG
CGTGCATGTGGCTGCCCATCTGGTGAATGCCCTCAACTTCTCAGTGAATTACAGTGAAGACTTTGTTGAA
CTGAATGCAGCAAGATACCGAGATGAGGATCCTAGAAAACTTCTCTTCACAACTGTTCCTGGCCTGACAG
GGGTCTGCATGGTGGTGGTGCTATTCCTCATGATCACAGCCTCTACATATGCAATAAGAGTTTCTAACTA
TGATATCTTCTGGTATACTCATAACCTCTTCTTTGTCTTCTACATGCTGCTGACGTTGCATGTTTCAGGA
GGGCTGCTGAAGTATCAAACTAATTTAGATACCCACCCTCCCGGCTGCATCAGTCTTAACCGAACCAGCT
CTCAGAATATTTCCTTACCAGAGTATTTCTCAGAACATTTTCATGAACCTTTCCCTGAAGGATTTTCAAA
ACCGGCAGAGTTTACCCAGCACAAATTTGTGAAGATTTGTATGGAAGAGCCCAGATTCCAAGCTAATTTT
CCACAGACTTGGCTTTGGATTTCTGGACCTTTGTGCCTGTACTGTGCCGAAAGACTTTACAGGTATATCC
GGAGCAATAAGCCAGTCACCATCATTTCGGTCATAAGTCATCCCTCAGATGTCATGGAAATCCGAATGGT
CAAAGAAAATTTTAAAGCAAGACCTGGTCAGTATATTACTCTACATTGTCCCAGTGTATCTGCATTAGAA
AATCATCCATTTACCCTCACAATGTGTCCAACTGAAACCAAAGCAACATTTGGGGTTCATCTTAAAATAG
TAGGAGACTGGACAGAACGATTTCGAGATTTACTACTGCCTCCATCTAGTCAAGACTCCGAAATTCTGCC
CTTCATTCAATCTAGAAATTATCCCAAGCTGTATATTGATGGTCCTTTTGGAAGTCCATTTGAGGAATCA
CTGAACTATGAGGTCAGCCTCTGCGTGGCTGGAGGCATTGGAGTAACTCCATTTGCATCAATACTCAACA
CCCTGTTGGATGACTGGAAACCATACAAGCTTAGAAGACTATACTTTATTTGGGTATGCAGAGATATCCA
GTCCTTCCGTTGGTTTGCAGATTTACTCTGTATGTTGCATAACAAGTTTTGGCAAGAGAACAGACCTGAC
TATGTCAACATCCAGCTGTACCTCAGTCAAACAGATGGGATACAGAAGATAATTGGAGAAAAATATCATG
CACTGAATTCAAGACTGTTTATAGGACGTCCTCGGTGGAAACTTTTGTTTGATGAAATAGCAAAATATAA
CAGAGGAAAAACAGTTGGTGTTTTCTGTTGTGGACCCAATTCACTATCCAAGACTCTTCATAAACTGAGT
AACCAGAACAACTCATATGGGACAAGATTTGAATACAATAAAGAGTCTTTCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208007 protein sequence
Red=Cloning site Green=Tags(s)

MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRASASVLNLNCSL
ILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSRTFHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFVE
LNAARYRDEDPRKLLFTTVPGLTGVCMVVVLFLMITASTYAIRVSNYDIFWYTHNLFFVFYMLLTLHVSG
GLLKYQTNLDTHPPGCISLNRTSSQNISLPEYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANF
PQTWLWISGPLCLYCAERLYRYIRSNKPVTIISVISHPSDVMEIRMVKENFKARPGQYITLHCPSVSALE
NHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEES
LNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPD
YVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLS
NQNNSYGTRFEYNKESFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016931
ORF Size 1734 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016931.4
RefSeq Size 4459 bp
RefSeq ORF 1737 bp
Locus ID 50507
UniProt ID Q9NPH5
Cytogenetics 11q14.3
Protein Families Druggable Genome, Transmembrane
MW 66.9 kDa
Gene Summary This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

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