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Lebercilin (LCA5) (NM_181714) Human Tagged ORF Clone

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SKU
RC207993
LCA5 (Myc-DDK-tagged)-Human Leber congenital amaurosis 5 (LCA5), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $638.00
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Bulk Requests & Clone Modifications

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Other products for "Lebercilin or NM_181714"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Lebercilin
Synonyms C6orf152
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207993 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAAAGAGCAGGAAGTCCAGGTACTGATCAAGAAAGAGAGGCAGGCAAACACCATTATTCTTACT
CATCTGATTTTGAAACGCCACAGTCTTCTGGCCGATCATCGCTGGTCAGTTCTTCACCTGCAAGTGTTAG
GAGAAAAAATCCTAAAAGACAAACTTCAGATGGCCAAGTACATCACCAAGCCCCTCGGAAACCAAGCCCT
AAGGGTCTACCAAACAGAAAGGGAGTCCGAGTGGGATTTCGCTCCCAGAGCCTCAATAGAGAGCCACTTC
GGAAAGATACTGATCTTGTTACAAAACGGATTCTGTCTGCAAGACTGCTAAAAATCAATGAGTTGCAGAA
TGAAGTATCTGAACTCCAGGTCAAGTTAGCTGAGCTGCTAAAAGAAAATAAATCTTTGAAAAGGCTTCAG
TACAGACAGGAGAAAGCCCTGAATAAGTTTGAAGATGCCGAAAATGAAATCTCACAACTTATATTTCGTC
ATAACAATGAGATTACAGCACTCAAAGAACGCTTAAGAAAATCTCAAGAGAAAGAACGGGCAACTGAGAA
AAGGGTAAAAGATACAGAAAGTGAACTATTTAGGACAAAATTTTCCTTACAGAAACTGAAAGAGATCTCT
GAAGCTAGACACCTACCTGAACGAGATGATTTGGCAAAGAAACTAGTTTCAGCAGAGTTAAAGTTAGATG
ACACCGAGAGAAGAATTAAGGAGCTATCGAAAAACCTTGAACTGAGTACTAACAGTTTCCAACGACAGTT
GCTTGCTGAAAGGAAAAGGGCATATGAGGCTCATGATGAAAATAAAGTTCTTCAAAAGGAGGTACAGCGA
CTATATCACAAATTAAAGGAAAAGGAGAGAGAACTGGATATAAAAAATATATATTCTAATCGTCTGCCAA
AGTCCTCTCCAAATAAAGAGAAAGAACTTGCATTAAGAAAAAATGCTGCATGCCAGAGTGATTTTGCAGA
CCTGTGTACAAAAGGAGTACAAACCATGGAAGACTTCAAGCCAGAAGAATATCCTTTAACTCCAGAAACA
ATTATGTGTTACGAAAACAAATGGGAAGAACCAGGACATCTTACTTTGGACTTGCAATCTCAAAAGCAAG
ACAGGCATGGAGAAGCAGGGATTCTAAACCCAATTATGGAAAGAGAAGAAAAATTTGTTACAGATGAAGA
ACTCCATGTCGTAAAACAGGAGGTTGAAAAGCTGGAGGATGAATGGGAAAGAGAAGAACTTGATAAAAAG
CAAAAAGAAAAGGCATCTTTACTGGAAAGAGAAGAAAAGCCAGAGTGGGAAACTGGAAGGTACCAACTAG
GAATGTATCCAATTCAGAATATGGATAAATTGCAAGGAGAGGAAGAAGAAAGACTGAAGAGAGAAATGCT
ACTTGCTAAACTGAATGAAATTGACAGAGAACTCCAAGATTCTCGAAATCTAAAATACCCTGTTTTGCCA
TTGTTACCTGATTTTGAATCAAAACTACACTCCCGAGAGAGAAGCCCCAAAACATACAGGTTCTCTGAAT
CCTCAGAGAGATTATTTAATGGGCATCATTTGCAAGACATCAGTTTCTCAACTCCAAAAGGAGAAGGTCA
GAATTCAGGAAATGTTAGAAGTCCAGCCTCCCCTAATGAGTTCGCATTTGGTAGCTACGTGCCTTCGTTT
GCAAAAACATCAGAGAGGTCAAATCCATTTAGTCAAAAAAGTAGTTTTTTGGATTTCCAAAGAAACAGTA
TGGAAAAACTTAGTAAAGATGGTGTAGATTTAATTACAAGAAAAGAGAAAAAAGCTAATTTGATGGAACA
GTTATTTGGTGCCAGTGGTAGCAGCACCATTTCCTCCAAAAGCAGTGGCCCAAATTCTGTGGCTTCCAGT
AAAGGAGACATTGACCCTCTAAATTTTCTCCCTGGGAATAAAGGCAGCAGAGATCAAGAACATGATGAAG
ATGAAGGCTTTTTCCTCAGTGAAGGAAGAAGTTTTAATCCAAATAGGCACCGATTAAAACATGCAGACGA
TAAACCAGCAGTAAAAGCAGCTGATTCTGTAGAAGATGAAATTGAAGAAGTAGCACTGAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207993 protein sequence
Red=Cloning site Green=Tags(s)

MGERAGSPGTDQEREAGKHHYSYSSDFETPQSSGRSSLVSSSPASVRRKNPKRQTSDGQVHHQAPRKPSP
KGLPNRKGVRVGFRSQSLNREPLRKDTDLVTKRILSARLLKINELQNEVSELQVKLAELLKENKSLKRLQ
YRQEKALNKFEDAENEISQLIFRHNNEITALKERLRKSQEKERATEKRVKDTESELFRTKFSLQKLKEIS
EARHLPERDDLAKKLVSAELKLDDTERRIKELSKNLELSTNSFQRQLLAERKRAYEAHDENKVLQKEVQR
LYHKLKEKERELDIKNIYSNRLPKSSPNKEKELALRKNAACQSDFADLCTKGVQTMEDFKPEEYPLTPET
IMCYENKWEEPGHLTLDLQSQKQDRHGEAGILNPIMEREEKFVTDEELHVVKQEVEKLEDEWEREELDKK
QKEKASLLEREEKPEWETGRYQLGMYPIQNMDKLQGEEEERLKREMLLAKLNEIDRELQDSRNLKYPVLP
LLPDFESKLHSRERSPKTYRFSESSERLFNGHHLQDISFSTPKGEGQNSGNVRSPASPNEFAFGSYVPSF
AKTSERSNPFSQKSSFLDFQRNSMEKLSKDGVDLITRKEKKANLMEQLFGASGSSTISSKSSGPNSVASS
KGDIDPLNFLPGNKGSRDQEHDEDEGFFLSEGRSFNPNRHRLKHADDKPAVKAADSVEDEIEEVALR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181714
ORF Size 2091 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181714.1, NP_859065.1
RefSeq Size 4729 bp
RefSeq ORF 2094 bp
Locus ID 167691
UniProt ID Q86VQ0
Cytogenetics 6q14.1
MW 80.5 kDa
Summary This gene encodes a protein that is thought to be involved in centrosomal or ciliary functions. Mutations in this gene cause Leber congenital amaurosis type V. Alternatively spliced transcript variants are described. provided by RefSeq, Oct 2009
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Documents
Resources
"NM_181714" in other vectors (4)
SKU Description Size Price
RC207993L3 Lenti ORF clone of Human Leber congenital amaurosis 5 (LCA5), transcript variant 1, Myc-DDK-tagged 10 ug
$938.00
RC207993L4 Lenti ORF clone of Human Leber congenital amaurosis 5 (LCA5), transcript variant 1, mGFP tagged 10 ug
$938.00
RG207993 LCA5 (tGFP-tagged) - Human Leber congenital amaurosis 5 (LCA5), transcript variant 1 10 ug
$789.00 $838.00
SC107321 LCA5 (untagged)-Human Leber congenital amaurosis 5 (LCA5), transcript variant 1 10 ug
$639.00

file-alt Citations

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