PPP1R3D (NM_006242) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207987L4
Lenti-ORF clone of PPP1R3D (mGFP-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D)
  $600.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPP1R3D
Synonyms PPP1R6
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207987).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006242
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006242.3
RefSeq Size 3481 bp
RefSeq ORF 900 bp
Locus ID 5509
UniProt ID O95685
Cytogenetics 20q13.33
Domains CBM_21
Protein Families Druggable Genome, Phosphatase
Protein Pathways Insulin signaling pathway
MW 32.6 kDa
Summary Phosphorylation of serine and threonine residues in proteins is a crucial step in the regulation of many cellular functions ranging from hormonal regulation to cell division and even short-term memory. The level of phosphorylation is controlled by the opposing actions of protein kinases and protein phosphatases. Protein phosphatase 1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases which have been identified in eukaryotic cells. PP1 associates with various regulatory subunits that dictate its subcellular localization and modulate its substrate specificity. Several subunits that target PP1 to glycogen have been identified. This gene encodes a glycogen-targeting subunit of PP1. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006242" in other vectors (4)
SKU Description Size Price
RC207987 PPP1R3D (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D) 10 ug
$289.00 $300.00
RC207987L3 Lenti-ORF clone of PPP1R3D (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D) 10 ug
$600.00
RG207987 PPP1R3D (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D) 10 ug
$500.00
SC125491 PPP1R3D (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D) 10 ug
$300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.