RNF149 (NM_173647) Human Tagged ORF Clone

CAT#: RC207979

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RNF149 (Myc-DDK-tagged)-Human ring finger protein 149 (RNF149)



  "NM_173647" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RNF149
Synonyms DNAPTP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207979 representing NM_173647
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGG
CCCTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGT
GGACCCGCAGACCAACCTGACGGTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAG
GAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGCGGAGACCTCGAGGGCTGCGCGCCCGACA
CGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCTCGTGGGGG
CTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAG
GAGCGCTACGGGAACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTA
GCTATCCAAAAGGAAGAGAAATTTTGGAGCTGGTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGT
TGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTGGCCATTGCCTTCATCACC
ATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGA
TTGGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCA
TGGAGAAAAGGGAATTGATGTTGATGCTGAAAATTGTGCAGTGTGTATTGAAAATTTCAAAGTAAAGGAT
ATTATTAGAATTCTGCCATGCAAGCATATTTTTCATAGAATATGCATTGACCCATGGCTTTTGGATCACC
GAACATGTCCAATGTGTAAACTTGATGTCATCAAAGCCCTAGGATATTGGGGAGAGCCTGGGGATGTACA
GGAGATGCCTGCTCCAGAATCTCCTCCTGGAAGGGATCCAGCTGCAAATTTGAGTCTAGCTTTACCAGAT
GATGACGGAAGTGATGAGAGCAGTCCACCATCAGCCTCCCCTGCTGAATCTGAGCCACAGTGTGATCCCA
GCTTTAAAGGAGATGCAGGAGAAAATACGGCATTGCTAGAAGCCGGCAGGAGTGACTCTCGGCATGGAGG
ACCCATCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207979 representing NM_173647
Red=Cloning site Green=Tags(s)

MAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPK
EGAHGLVGVPWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNE
ERYGNITLPMSHAGTGNIVVIMISYPKGREILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFIT
MMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGEKGIDVDAENCAVCIENFKVKD
IIRILPCKHIFHRICIDPWLLDHRTCPMCKLDVIKALGYWGEPGDVQEMPAPESPPGRDPAANLSLALPD
DDGSDESSPPSASPAESEPQCDPSFKGDAGENTALLEAGRSDSRHGGPIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_173647
ORF Size 1200 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_173647.2, NP_775918.1
RefSeq Size 2452 bp
RefSeq ORF 1203 bp
Locus ID 284996
UniProt ID Q8NC42
Cytogenetics 2q11.2
Protein Families Druggable Genome, Transmembrane
MW 43 kDa
Gene Summary E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.[UniProtKB/Swiss-Prot Function]

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