N WASP (WASL) (NM_003941) Human Tagged ORF Clone

CAT#: RC207967

WASL (Myc-DDK-tagged)-Human Wiskott-Aldrich syndrome-like (WASL)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY WASL (Cat# RC207967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WASL (Cat# TA811282)(1:2000). Positive lysates LY401293 (100ug) and LC401293 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401293) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207967 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WASL protein (Cat# TP307967). The protein was produced from HEK293T cells transfected with WASL cDNA clone (Cat# RC207967) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N WASP
• Synonyms: N-WASP; NWASP; WASPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207967 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTGGGGTCCCTGTTGCTCACCC
  CGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAATGTGTGACTATGTCTTCAGCAGTGGTGCA
  GTTATATGCAGCAGATCGGAACTGTATGTGGTCAAAGAAGTGCAGTGGTGTTGCTTGTCTTGTTAAGGAC
  AATCCACAGAGATCTTATTTTTTAAGAATATTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGC
  TATACAATAACTTTGTATATAATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGT
  TGCTCTTAATTTTGCCAATGAAGAAGAAGCAAAAAAATTTCGAAAAGCAGTTACAGACCTTTTGGGCCGT
  CGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTACCCATGGCTACAGTTGATA
  TAAAAAATCCAGAAATCACAACAAATAGATTTTATGGTCCACAAGTCAACAACATCTCCCATACCAAAGA
  AAAGAAGAAGGGAAAAGCTAAAAAGAAGAGATTAACCAAGGCAGATATAGGAACACCAAGCAATTTCCAG
  CACATTGGACATGTTGGTTGGGATCCAAATACAGGCTTTGATCTGAATAATTTGGATCCAGAATTGAAGA
  ATCTTTTCGATATGTGTGGAATCTCAGAGGCACAACTTAAAGACAGAGAAACATCAAAAGTTATATATGA
  CTTTATTGAAAAAACAGGAGGTGTTGAAGCTGTTAAAAATGAACTGCGGAGGCAAGCACCACCACCTCCA
  CCACCATCAAGGGGAGGGCCACCTCCTCCTCCTCCCCCTCCACACAACTCAGGTCCTCCTCCTCCTCCTG
  CTAGGGGAAGAGGCGCTCCTCCCCCACCACCTTCAAGAGCTCCCACAGCTGCACCTCCACCACCGCCTCC
  TTCCAGGCCAAGTGTAGCAGTCCCTCCACCACCGCCAAATAGGATGTACCCTCCTCCACCTCCAGCCCTT
  CCCTCCTCAGCACCTTCAGGGCCTCCACCACCACCTCCATCTGTGTTGGGGGTAGGGCCAGTGGCACCAC
  CCCCACCGCCTCCACCTCCACCTCCTCCTGGGCCACCGCCCCCGCCTGGCCTGCCTTCTGATGGGGACCA
  TCAGGTTCCAACTACTGCAGGAAACAAAGCAGCTCTTTTAGATCAAATTAGAGAGGGTGCTCAGCTAAAA
  AAAGTGGAGCAGAACAGTCGGCCAGTGTCCTGCTCTGGACGAGATGCACTGTTAGACCAGATACGACAGG
  GTATCCAACTAAAATCTGTGGCTGATGGCCAAGAGTCTACACCACCAACACCTGCACCCACTTCAGGAAT
  TGTGGGTGCATTAATGGAAGTGATGCAGAAAAGGAGCAAAGCCATTCATTCTTCAGATGAAGATGAAGAT
  GAAGATGATGAAGAAGATTTTGAGGATGATGATGAGTGGGAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207967 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKD
  NPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGR
  RQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQ
  HIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPP
  PPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPAL
  PSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLK
  KVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDED
  EDDEEDFEDDDEWED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003941
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003941.4
• RefSeq Size: 4447 bp
• RefSeq ORF: 1518 bp
• Locus ID: 8976
• UniProt ID: O00401
• Cytogenetics: 7q31.32
• Domains: PBD, WH1, WH2
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• MW: 54.8 kDa
• Gene Summary: This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]