N WASP (WASL) (NM_003941) Human Tagged ORF Clone

CAT#: RC207967

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WASL (Myc-DDK-tagged)-Human Wiskott-Aldrich syndrome-like (WASL)



  "NM_003941" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol WASL
Synonyms N-WASP; NWASP; WASPB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207967 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTGGGGTCCCTGTTGCTCACCC
CGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAATGTGTGACTATGTCTTCAGCAGTGGTGCA
GTTATATGCAGCAGATCGGAACTGTATGTGGTCAAAGAAGTGCAGTGGTGTTGCTTGTCTTGTTAAGGAC
AATCCACAGAGATCTTATTTTTTAAGAATATTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGC
TATACAATAACTTTGTATATAATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGT
TGCTCTTAATTTTGCCAATGAAGAAGAAGCAAAAAAATTTCGAAAAGCAGTTACAGACCTTTTGGGCCGT
CGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTACCCATGGCTACAGTTGATA
TAAAAAATCCAGAAATCACAACAAATAGATTTTATGGTCCACAAGTCAACAACATCTCCCATACCAAAGA
AAAGAAGAAGGGAAAAGCTAAAAAGAAGAGATTAACCAAGGCAGATATAGGAACACCAAGCAATTTCCAG
CACATTGGACATGTTGGTTGGGATCCAAATACAGGCTTTGATCTGAATAATTTGGATCCAGAATTGAAGA
ATCTTTTCGATATGTGTGGAATCTCAGAGGCACAACTTAAAGACAGAGAAACATCAAAAGTTATATATGA
CTTTATTGAAAAAACAGGAGGTGTTGAAGCTGTTAAAAATGAACTGCGGAGGCAAGCACCACCACCTCCA
CCACCATCAAGGGGAGGGCCACCTCCTCCTCCTCCCCCTCCACACAACTCAGGTCCTCCTCCTCCTCCTG
CTAGGGGAAGAGGCGCTCCTCCCCCACCACCTTCAAGAGCTCCCACAGCTGCACCTCCACCACCGCCTCC
TTCCAGGCCAAGTGTAGCAGTCCCTCCACCACCGCCAAATAGGATGTACCCTCCTCCACCTCCAGCCCTT
CCCTCCTCAGCACCTTCAGGGCCTCCACCACCACCTCCATCTGTGTTGGGGGTAGGGCCAGTGGCACCAC
CCCCACCGCCTCCACCTCCACCTCCTCCTGGGCCACCGCCCCCGCCTGGCCTGCCTTCTGATGGGGACCA
TCAGGTTCCAACTACTGCAGGAAACAAAGCAGCTCTTTTAGATCAAATTAGAGAGGGTGCTCAGCTAAAA
AAAGTGGAGCAGAACAGTCGGCCAGTGTCCTGCTCTGGACGAGATGCACTGTTAGACCAGATACGACAGG
GTATCCAACTAAAATCTGTGGCTGATGGCCAAGAGTCTACACCACCAACACCTGCACCCACTTCAGGAAT
TGTGGGTGCATTAATGGAAGTGATGCAGAAAAGGAGCAAAGCCATTCATTCTTCAGATGAAGATGAAGAT
GAAGATGATGAAGAAGATTTTGAGGATGATGATGAGTGGGAAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207967 protein sequence
Red=Cloning site Green=Tags(s)

MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKD
NPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGR
RQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQ
HIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPP
PPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPAL
PSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLK
KVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDED
EDDEEDFEDDDEWED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003941
ORF Size 1515 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003941.4
RefSeq Size 4447 bp
RefSeq ORF 1518 bp
Locus ID 8976
UniProt ID O00401
Cytogenetics 7q31.32
Domains PBD, WH1, WH2
Protein Families Druggable Genome
Protein Pathways Adherens junction, Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
MW 54.8 kDa
Gene Summary This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]

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